##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.10uM.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24182374
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	61-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.23356768032783	37.0	35.0	38.0	33.0	39.0
2	35.4697154216538	35.0	34.0	38.0	33.0	39.0
3	35.5811940134579	36.0	35.0	37.0	33.0	39.0
4	35.709936294922905	36.0	35.0	38.0	33.0	39.0
5	35.58435759863775	36.0	35.0	38.0	33.0	39.0
6	35.56003587571675	36.0	35.0	37.0	33.0	39.0
7	35.471962057984875	35.0	34.0	37.0	33.0	39.0
8	35.655382469893155	36.0	35.0	38.0	33.0	39.0
9	35.631585716108766	36.0	35.0	38.0	33.0	39.0
10-14	35.596523525771296	36.0	35.0	37.8	33.0	39.0
15-19	35.545290400355235	36.0	35.0	38.0	33.0	39.0
20-24	35.51180856767826	36.0	35.0	38.0	33.0	39.0
25-29	35.46107512025081	36.0	35.0	38.0	33.0	39.0
30-34	35.42834037716893	36.0	34.4	38.0	33.0	39.0
35-39	35.38946974354131	36.0	34.0	38.0	32.6	39.0
40-44	35.344919923908215	36.0	34.0	38.0	32.0	39.0
45-49	35.29650251873534	36.0	34.0	38.0	32.0	39.0
50-54	35.24666149816391	36.0	34.0	38.0	32.0	39.0
55-59	35.2091473814771	36.0	34.0	38.0	32.0	39.0
60-64	35.14685446852121	36.0	34.0	37.8	32.0	39.0
65-69	35.08635485093297	36.0	34.0	37.6	32.0	39.0
70-74	35.02019167256633	35.8	34.0	37.0	31.4	39.0
75-79	34.95327819189434	35.2	34.0	37.0	31.0	39.0
80-84	34.88121148607741	35.0	34.0	37.0	31.0	39.0
85-89	34.785816065428264	35.0	34.0	37.0	31.0	39.0
90-94	34.71031990043785	35.0	34.0	37.0	30.0	39.0
95-99	34.64349100814295	35.0	34.0	37.0	30.0	39.0
100-104	34.58200888626278	35.0	34.0	37.0	30.0	39.0
105-109	34.45572256315173	35.0	34.0	37.0	30.0	39.0
110-114	34.379071977035345	35.0	34.0	37.0	29.4	39.0
115-119	34.28363129277252	35.0	34.0	37.0	29.0	39.0
120-124	34.218105864992346	35.0	34.0	37.0	29.0	39.0
125-129	34.065747906514524	35.0	34.0	37.0	28.0	39.0
130-134	33.97118341836456	35.0	33.2	37.0	28.0	39.0
135-139	33.85213425915461	35.0	33.0	37.0	27.8	39.0
140-144	33.706470002519026	35.0	33.0	37.0	27.0	39.0
145-149	33.67807416682374	35.0	33.0	37.0	27.0	39.0
150	34.69200395478636	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	31.0
21	490.0
22	3550.0
23	17165.0
24	55028.0
25	122268.0
26	213141.0
27	328274.0
28	446686.0
29	562343.0
30	695398.0
31	879491.0
32	1149487.0
33	1581250.0
34	2377892.0
35	4154134.0
36	1.0336409E7
37	1259334.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.85002754485561	19.04089730809721	17.11532953712485	28.993745609922332
2	23.412954551648014	25.095337004354366	33.96580641610317	17.52590202789445
3	20.5177964861822	28.664351094297754	31.47148842970566	19.346363989814392
4	23.989471071581843	33.31630974895602	23.58610309808008	19.10811608138205
5	24.8264315619625	35.28004343254072	23.45102083575006	16.442504169746723
6	19.690027124714884	38.79916008246337	24.05514032658663	17.455672466235118
7	20.2374589029183	21.266787123547093	39.153955686898236	19.341798286636376
8	22.00275704941128	23.65229319503536	30.228504447081995	24.11644530847137
9	23.148566803242726	23.331791163266267	29.984901399672342	23.534740633818664
10-14	22.70074311149104	29.59268267044418	26.898291292658033	20.808282925406747
15-19	22.619980982843124	28.474470703331278	28.530791062945266	20.374757250880332
20-24	22.638099168782126	28.622215152242177	28.23293986021768	20.506745818758013
25-29	22.675765724570667	28.608292766762705	28.209230555139765	20.506710953526866
30-34	22.67525678000018	28.385106441576003	28.404647947302443	20.534988831121378
35-39	22.766058452325648	28.42169342017454	28.25082682122111	20.561421306278696
40-44	22.998264769207523	28.191779682176776	28.230062110527278	20.57989343808842
45-49	22.996413007258923	28.151839848312658	28.249539106458283	20.602208037970136
50-54	23.051938763761893	28.108432431889707	28.24896040602929	20.59066839831911
55-59	23.17814473614662	27.933686973278032	28.25594400496778	20.632224285607563
60-64	23.052862131390295	27.887634056630684	28.349375334680754	20.710128477298262
65-69	23.078770195179484	27.916495884517907	28.385693949881848	20.619039970420765
70-74	23.28813206970669	27.831483652396017	28.283200204737895	20.597184073159394
75-79	23.17480323733901	27.827633847128517	28.371907305686513	20.62565560984596
80-84	23.289467840394053	27.912042069454166	28.187613601561416	20.610876488590364
85-89	23.284230700491925	27.87055263841074	28.28974926633126	20.555467394766076
90-94	23.255665790098547	27.893679911841478	28.273401483541694	20.577252814518282
95-99	23.203200235718874	27.990971035507506	28.309512266261223	20.4963164625124
100-104	23.386747027216064	27.956258499901487	28.16558788259172	20.49140659029073
105-109	23.19513203615009	28.003902102890972	28.371906761697556	20.429059099261384
110-114	23.27188171001285	27.97605768189658	28.304327185374976	20.44773342271559
115-119	23.34466379352729	27.977109845723202	28.376681324393555	20.301545036355957
120-124	23.37462738307295	27.988920153456537	28.397017906296202	20.239434557174306
125-129	23.307127249877933	28.192560516169436	28.258004289276172	20.24230794467646
130-134	23.439854888744275	28.1700103448971	28.221898688332193	20.168236078026432
135-139	23.26603463553807	28.18009585123998	28.42678124646975	20.127088266752203
140-144	23.22267925168184	28.24333786303549	28.333753796459778	20.200229088822898
145-149	23.075972872028633	28.095799736636252	28.46948616192878	20.358741229406334
150	23.643634693350677	26.958754448614336	28.58950658646937	20.808104271565618
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.5
6	1.0
7	1.5
8	6.0
9	15.5
10	25.5
11	32.5
12	48.5
13	84.5
14	143.5
15	246.0
16	416.5
17	680.0
18	1130.5
19	1853.0
20	2892.5
21	4656.5
22	7269.5
23	11067.0
24	16236.5
25	24226.5
26	44540.0
27	62236.5
28	75158.0
29	103115.0
30	137851.5
31	173618.0
32	221930.5
33	285531.5
34	359837.5
35	454061.0
36	576386.5
37	716777.5
38	862097.5
39	1027232.0
40	1209958.0
41	1376077.0
42	1522619.5
43	1620657.0
44	1653466.0
45	1656832.5
46	1608099.5
47	1484505.5
48	1319657.5
49	1128429.5
50	938602.5
51	771875.0
52	620131.0
53	511918.0
54	423513.0
55	331303.0
56	247015.0
57	174621.0
58	121503.0
59	81399.5
60	57206.5
61	43904.5
62	32274.0
63	21900.0
64	14963.5
65	10298.0
66	6945.0
67	4969.5
68	3785.0
69	2788.0
70	2116.0
71	1651.5
72	1288.0
73	1118.5
74	1207.5
75	1162.5
76	780.5
77	362.0
78	164.0
79	73.0
80	22.0
81	9.0
82	3.5
83	5.0
84	6.0
85	4.0
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.135243297452929E-6
3	6.616389275924687E-5
4	9.759174181988915E-4
5	0.15061796662312807
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	7.443437935415274E-6
25-29	0.05939367243265694
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.27048659490586E-7
55-59	8.27048659490586E-7
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.27179366996688E-7
110-114	0.0
115-119	8.272804121060972E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	0.0
64-65	2.0
66-67	8.0
68-69	10.0
70-71	15.0
72-73	31.0
74-75	26.0
76-77	52.0
78-79	54.0
80-81	66.0
82-83	78.0
84-85	123.0
86-87	155.0
88-89	186.0
90-91	211.0
92-93	234.0
94-95	274.0
96-97	346.0
98-99	375.0
100-101	435.0
102-103	438.0
104-105	441.0
106-107	505.0
108-109	507.0
110-111	574.0
112-113	636.0
114-115	633.0
116-117	713.0
118-119	754.0
120-121	846.0
122-123	945.0
124-125	1092.0
126-127	1218.0
128-129	1397.0
130-131	1632.0
132-133	1990.0
134-135	2632.0
136-137	3445.0
138-139	6065.0
140-141	9703.0
142-143	14417.0
144-145	66407.0
146-147	740292.0
148-149	1220584.0
150-151	2.2101826E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.64903807218977
#Duplication Level	Percentage of total
1	28.608190595826244
2	10.895103562715327
3	6.750850618522043
4	4.685797383972563
5	3.699207511892353
6	2.8476801146113675
7	2.318049325178488
8	1.9563172573679715
9	1.6382005912862774
>10	19.648931497188308
>50	5.652972069291246
>100	8.110264073145016
>500	1.5836650074466496
>1k	1.4181586851490053
>5k	0.18661170640701485
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.13524329745293E-6	0.0	0.0	0.0	0.0013150073685900317	0.0
2	6.616389275924688E-5	0.0	0.0	4.13524329745293E-6	0.010371190190011948	0.0
3	2.6879081433444046E-4	0.0	0.0	4.13524329745293E-6	0.01237678318927662	4.13524329745293E-6
4	3.8044238336566955E-4	0.0	0.0	8.27048659490586E-6	0.014750412842014601	4.13524329745293E-6
5	4.2179481634019886E-4	0.0	0.0	1.240572989235879E-5	0.017765005205857786	4.13524329745293E-6
6	5.044996822892575E-4	0.0	0.0	1.654097318981172E-5	0.019865708800963874	4.13524329745293E-6
7	5.541226018586926E-4	0.0	0.0	2.067621648726465E-5	0.022404748185599975	4.13524329745293E-6
8	6.368274678077513E-4	0.0	0.0	2.067621648726465E-5	0.02795837993407926	4.13524329745293E-6
9	9.387002285218151E-4	0.0	0.0	2.067621648726465E-5	0.039156618783581794	4.13524329745293E-6
10-11	0.0013749683964030992	4.13524329745293E-6	0.0	2.2743838135991114E-5	0.04826862738951933	4.13524329745293E-6
12-13	0.0014039150994852698	4.13524329745293E-6	0.0	2.894670308217051E-5	0.05455419720164778	4.13524329745293E-6
14-15	0.0014700789922445167	4.13524329745293E-6	0.0	3.308194637962344E-5	0.07192015142930136	6.202864946179395E-6
16-17	0.0015093638035703193	6.202864946179395E-6	0.0	3.721718967707637E-5	0.0868773264361886	1.240572989235879E-5
18-19	0.0017657488880124012	1.240572989235879E-5	0.0	3.721718967707637E-5	0.09309259711226037	1.654097318981172E-5
20-21	0.0021048388384035414	1.240572989235879E-5	0.0	3.721718967707637E-5	0.10107154905469579	1.654097318981172E-5
22-23	0.002437725923848502	1.240572989235879E-5	0.0	4.342005462325576E-5	0.10856047466638305	1.654097318981172E-5
24-25	0.002472875491876852	1.240572989235879E-5	0.0	5.375816286688809E-5	0.1139486966829642	1.8608594838538183E-5
26-27	0.0025266336547437405	1.240572989235879E-5	0.0	5.375816286688809E-5	0.11904331642542622	2.067621648726465E-5
28-29	0.0027313281979676604	1.240572989235879E-5	0.0	5.375816286688809E-5	0.12465277395842111	2.067621648726465E-5
30-31	0.0029835780391122887	1.240572989235879E-5	0.0	5.375816286688809E-5	0.12814085167982267	2.067621648726465E-5
32-33	0.0030207952287893652	1.240572989235879E-5	0.0	5.375816286688809E-5	0.13207346805570042	2.067621648726465E-5
34-35	0.003134514419469321	1.240572989235879E-5	0.0	5.789340616434102E-5	0.1353547836122293	2.894670308217051E-5
36-37	0.0031613935009027648	1.240572989235879E-5	0.0	5.789340616434102E-5	0.1375816121279077	3.1014324730896976E-5
38-39	0.0032689098266365413	1.240572989235879E-5	0.0	5.789340616434102E-5	0.1396513013982829	3.928481132580283E-5
40-41	0.0033516146925855997	1.240572989235879E-5	0.0	6.616389275924688E-5	0.14195876715826164	4.342005462325576E-5
42-43	0.00362040550692004	1.240572989235879E-5	0.0	7.029913605669981E-5	0.1440739441049088	5.5825784515614555E-5
44-45	0.004067011783044957	1.4473351541085255E-5	0.0	7.443437935415274E-5	0.14652407575864967	6.823151440797335E-5
46-47	0.004478468491141523	1.654097318981172E-5	0.0	7.856962265160568E-5	0.1486764698949739	9.304297419269092E-5
48-49	0.004681095412716716	1.654097318981172E-5	0.0	7.856962265160568E-5	0.15066965716434622	1.0751632573377618E-4
50-51	0.005100822607408189	1.654097318981172E-5	0.0	7.856962265160568E-5	0.15290889140991698	1.240572989235879E-4
52-53	0.005439912557799329	1.654097318981172E-5	0.0	9.097535254396446E-5	0.15507782651943106	1.5093638035703196E-4
54-55	0.005714906237079949	1.654097318981172E-5	0.0	9.924583913887032E-5	0.15684357540744345	1.7781546179047598E-4
56-57	0.005898924563816604	2.067621648726465E-5	0.0	9.924583913887032E-5	0.15850594321301953	2.129650298188259E-4
58-59	0.006000238024604202	2.067621648726465E-5	0.0	9.924583913887032E-5	0.15975065144555287	2.501822194959023E-4
60-61	0.006178053486394677	2.067621648726465E-5	0.0	1.0131346078759678E-4	0.16109047027392762	2.97737517416611E-4
62-63	0.006852098143879505	2.067621648726465E-5	0.0	1.0338108243632325E-4	0.16239927477757146	3.618337885271314E-4
64-65	0.00768534966831627	2.067621648726465E-5	0.0	1.0544870408504971E-4	0.16350545235964012	4.0732146479911357E-4
66-67	0.008191916972254253	2.2743838135991114E-5	0.0	1.1165156903122911E-4	0.16465504999633204	4.4453865447619E-4
68-69	0.0087501748174104	2.481145978471758E-5	0.0	1.2819254222104083E-4	0.1657384837402647	4.920939523968986E-4
70-71	0.009070656172963001	2.481145978471758E-5	0.0	1.364630288159467E-4	0.16681984986254866	5.64460710102325E-4
72-73	0.00971988937066311	2.481145978471758E-5	0.0	1.426658937621261E-4	0.16775234722612428	6.409627111052041E-4
74-75	0.01014168418700331	2.481145978471758E-5	0.0	1.488687587083055E-4	0.16870965604948462	6.616389275924687E-4
76-77	0.010472503650799546	2.481145978471758E-5	0.0	1.488687587083055E-4	0.16982617173979692	7.112618471619039E-4
78-79	0.010728888735241627	2.481145978471758E-5	0.0	1.488687587083055E-4	0.17094061980846048	7.340056852978951E-4
80-81	0.011022491009360785	2.481145978471758E-5	0.0	1.5093638035703196E-4	0.17210882603999095	7.877638481647831E-4
82-83	0.011220982687638527	2.481145978471758E-5	0.0	1.5300400200575842E-4	0.17324188270349306	8.291162811393125E-4
84-85	0.011493908745270419	2.481145978471758E-5	0.0	1.5300400200575842E-4	0.17434185742061553	8.601306058702094E-4
86-87	0.011580748854516931	3.308194637962344E-5	0.0	1.5300400200575842E-4	0.17535085678519402	8.828744440062006E-4
88-89	0.011603492692652921	3.308194637962344E-5	0.0	1.5300400200575842E-4	0.17643222290747798	9.428354718192681E-4
90-91	0.01162210128749146	3.308194637962344E-5	0.0	1.5713924530321132E-4	0.17752599475965428	9.945260130374296E-4
92-93	0.011624168909140186	3.308194637962344E-5	0.0	1.7161259684429658E-4	0.17861563136853315	0.0010462165542555913
94-95	0.011624168909140186	3.308194637962344E-5	0.0	1.819507050879289E-4	0.17953985824551388	0.0010937718521763
96-97	0.011628304152437638	3.308194637962344E-5	0.0	1.819507050879289E-4	0.18071013209869302	0.0011268537985559234
98-99	0.011659318477168536	3.308194637962344E-5	0.0	1.8401832673665538E-4	0.18193623173638784	0.001182679583071538
100-101	0.011665521342114715	3.308194637962344E-5	0.0	1.9228881333156124E-4	0.1832388333750855	0.0012219643943973408
102-103	0.011667588963763442	3.308194637962344E-5	0.0	2.005592999264671E-4	0.1842312917664742	0.0012777901789129552
104-105	0.011677927072007075	3.308194637962344E-5	0.0	2.2123551641373176E-4	0.18534573983513777	0.0013522245582671083
106-107	0.01168619755860198	3.308194637962344E-5	0.0	2.2743838135991115E-4	0.18627203433376724	0.0014204560726750815
108-109	0.01169860328849434	3.308194637962344E-5	0.0	2.3157362465736408E-4	0.18735340045605117	0.0014907552087317813
110-111	0.011719279504981604	3.308194637962344E-5	0.0	2.4397935454972288E-4	0.18878212701532116	0.001565189588085934
112-113	0.011766834802902313	3.308194637962344E-5	0.0	2.481145978471758E-4	0.18991518367882326	0.0016313534808451808
114-115	0.011783375776092123	3.721718967707637E-5	0.0	2.481145978471758E-4	0.1909345211516454	0.0016789087787658897
116-117	0.011789578641038303	3.721718967707637E-5	0.0	2.481145978471758E-4	0.19208618640998604	0.0017223288333891454
118-119	0.011797849127633209	3.721718967707637E-5	0.0	2.543174627933552E-4	0.19303729236840023	0.001800898456040751
120-121	0.011818525344120475	4.342005462325576E-5	0.0	2.6258794938826104E-4	0.19419722811333578	0.0018505213756101861
122-123	0.011830931074012833	4.548767627198223E-5	0.0	2.708584359831669E-4	0.1955494526716029	0.0019373614848566976
124-125	0.011835066317310286	5.1690541218161624E-5	0.0	2.770613009293463E-4	0.19673626749797185	0.0019725110528850476
126-127	0.011839201560607738	5.375816286688809E-5	0.0	2.853317875242522E-4	0.197801092647066	0.0020386749456442945
128-129	0.011847472047202644	5.375816286688809E-5	2.067621648726465E-6	2.9360227411915805E-4	0.19906440947443788	0.0021110417033497206
130-131	0.01185574253379755	5.375816286688809E-5	4.13524329745293E-6	2.97737517416611E-4	0.20026569765234795	0.002154461757972977
132-133	0.01185574253379755	5.375816286688809E-5	4.13524329745293E-6	3.018727607140639E-4	0.20132018469319846	0.002195814190947506
134-135	0.011859877777095003	5.375816286688809E-5	6.202864946179395E-6	3.0600800401151684E-4	0.2022961021113973	0.0022392342455707613
136-137	0.01186194539874373	5.375816286688809E-5	8.27048659490586E-6	3.142784906064227E-4	0.20362765045317716	0.002297127651735103
138-139	0.011864013020392456	5.789340616434102E-5	8.27048659490586E-6	3.1841373390387563E-4	0.20463871743940443	0.002334344841412179
>>END_MODULE
