##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.1uM.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24035964
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	67-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.02173676079728	37.0	35.0	38.0	33.0	39.0
2	35.17911971410841	35.0	34.0	38.0	32.0	39.0
3	35.39049663246292	36.0	34.0	38.0	32.0	39.0
4	35.398419510030884	36.0	35.0	38.0	32.0	39.0
5	35.31192304165541	36.0	35.0	38.0	32.0	39.0
6	35.21760845539625	36.0	34.0	37.0	33.0	39.0
7	35.0807986315839	35.0	34.0	37.0	33.0	39.0
8	35.245331995005486	35.0	34.0	37.0	33.0	39.0
9	35.21256813331889	35.0	34.0	37.0	33.0	39.0
10-14	35.255537535336636	35.4	34.2	37.4	32.2	39.0
15-19	35.23078382876593	36.0	34.0	37.0	32.0	39.0
20-24	35.234098145595496	36.0	34.0	37.0	32.0	39.0
25-29	35.21897914308742	36.0	34.0	37.0	32.0	39.0
30-34	35.19554458477305	36.0	34.0	37.0	32.0	39.0
35-39	35.163785442514396	35.8	34.0	37.0	32.0	39.0
40-44	35.14353958093797	35.2	34.0	37.0	32.0	39.0
45-49	35.12802809989232	35.6	34.0	37.0	32.0	39.0
50-54	35.10146505461566	35.0	34.0	37.0	32.0	39.0
55-59	35.07643885637373	35.0	34.0	37.0	32.0	39.0
60-64	35.05687660374262	35.0	34.0	37.0	32.0	39.0
65-69	35.020793932036526	35.0	34.0	37.0	32.0	39.0
70-74	35.01006636695655	35.0	34.0	37.0	32.0	39.0
75-79	34.979566851843444	35.0	34.0	37.0	32.0	39.0
80-84	34.957226341923366	35.0	34.0	37.0	32.0	39.0
85-89	34.928082352504504	35.0	34.0	37.0	32.0	39.0
90-94	34.89915207475819	35.0	34.0	37.0	31.0	39.0
95-99	34.862613418088415	35.0	34.0	37.0	31.0	39.0
100-104	34.83352461672206	35.0	34.0	37.0	31.0	39.0
105-109	34.80963998164198	35.0	34.0	37.0	31.0	39.0
110-114	34.764950580295036	35.0	34.0	37.0	31.0	39.0
115-119	34.74119646858895	35.0	34.0	37.0	31.0	39.0
120-124	34.70374204756645	35.0	34.0	37.0	31.0	39.0
125-129	34.661304815759806	35.0	34.0	37.0	31.0	39.0
130-134	34.62749150501078	35.0	34.0	37.0	31.0	39.0
135-139	34.58720698413211	35.0	34.0	37.0	30.0	39.0
140-144	34.541754222991905	35.0	34.0	37.0	30.0	39.0
145-149	34.55233167312282	35.0	34.0	37.0	30.2	39.0
150	35.22060979101814	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	46.0
23	836.0
24	7156.0
25	35186.0
26	108876.0
27	235410.0
28	385892.0
29	526020.0
30	680324.0
31	887387.0
32	1196244.0
33	1685594.0
34	2565842.0
35	4516523.0
36	1.0138465E7
37	1066148.0
38	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.623405826369186	11.854560940430764	14.174047689537229	38.34798554366282
2	21.809225041373637	12.252046298531384	34.9470944179271	30.99163424216788
3	20.216402861079956	16.642811297588818	27.847022812078926	35.293763029252304
4	23.88167885861618	24.2916297460547	22.517393425458668	29.30929796987045
5	24.234305171915114	31.0440635757904	24.412863703067124	20.30876754922737
6	18.77978625084759	37.41277087988361	25.603675644676922	18.20376722459188
7	14.762353873706976	26.943233553980583	39.922838435881545	18.3715741364309
8	16.987281391770264	26.38631714700976	32.13759921344015	24.488802247779834
9	17.683525835522296	24.654988836625986	33.44283829949262	24.218647028359097
10-14	18.957696199838615	30.72390180718585	27.60180040262643	22.716601590349107
15-19	19.122722301889418	29.14349812419159	28.497666291742963	23.236113282176028
20-24	19.204443207550035	29.299148838875727	28.142226629273377	23.35418132430086
25-29	19.36211543672036	29.375113050130082	27.975916688130486	23.286854825019077
30-34	19.252514999498914	29.31272850639862	27.936912255506662	23.497844238595803
35-39	19.335413717620607	29.1092818661874	28.10728567547588	23.44801874071612
40-44	19.333087828617348	29.206730062156204	28.02049828193434	23.4396838272921
45-49	19.44527435981434	29.0072440753043	28.025572395445845	23.52190916943551
50-54	19.422795123556217	29.11274019956774	28.011867343882297	23.45259733299375
55-59	19.52561144018473	29.045407244223053	27.963565953176456	23.465415362415765
60-64	19.542213507629477	28.953352933234427	27.87856163643459	23.625871922701506
65-69	19.531086663738936	28.852001728146785	28.068758188473343	23.548153419640933
70-74	19.632244359286226	28.861148779935725	27.99830417273574	23.508302688042313
75-79	19.500033603884894	28.866932834508617	28.011626802718204	23.621406758888284
80-84	19.70178884754244	28.708785957950774	27.915045655415256	23.674379539091532
85-89	19.67226672377916	28.79769343762682	28.0266905774062	23.503349261187818
90-94	19.82451307204047	28.65887251756894	27.92715068554566	23.589463724844933
95-99	19.859386836327133	28.678022878388067	27.963674649804688	23.49891563548011
100-104	19.897556789643993	28.56844239108392	27.956110188526022	23.57789063074606
105-109	19.82606672394967	28.537672724490758	28.007695252785386	23.628565298774188
110-114	19.89285990073173	28.575836178065124	27.92918947777648	23.60211444342667
115-119	19.974889573855027	28.504956139176414	28.000753008225924	23.519401278742638
120-124	19.991095131558673	28.358928741630386	28.089917868540702	23.560058258270242
125-129	20.08806569425916	28.363608200678197	28.01590044245711	23.532425662605533
130-134	19.981159914427977	28.520442555522408	27.980229119433588	23.518168410616035
135-139	20.066728231964245	28.33704989821653	28.009594506232588	23.586627363586636
140-144	20.156596753260423	28.258370737778364	28.086480371392437	23.49855213756878
145-149	19.828957019679976	28.18165182564606	28.36825548393306	23.621135670740912
150	20.218155832781807	27.13911870980523	28.396613694904506	24.246111762508455
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.0
7	7.0
8	8.5
9	13.5
10	27.0
11	45.0
12	81.5
13	146.5
14	238.5
15	417.5
16	800.0
17	1459.0
18	2891.0
19	5364.5
20	6830.5
21	8648.5
22	12744.5
23	18586.0
24	27465.0
25	39126.0
26	53259.0
27	72445.0
28	99650.0
29	136226.5
30	181198.5
31	233666.5
32	288997.0
33	348261.0
34	419765.0
35	507433.0
36	606896.0
37	719969.5
38	846382.0
39	984923.0
40	1131926.0
41	1275811.0
42	1406829.0
43	1510799.0
44	1584252.5
45	1609873.0
46	1560679.5
47	1464248.5
48	1321879.5
49	1144943.5
50	965325.5
51	789785.5
52	632967.0
53	506984.5
54	411668.0
55	320297.0
56	237122.5
57	167901.0
58	111214.0
59	72594.5
60	51306.0
61	39533.5
62	29671.0
63	20475.0
64	13096.0
65	8735.0
66	5868.5
67	3884.5
68	2410.0
69	1594.0
70	1607.0
71	1854.0
72	1839.5
73	1324.0
74	783.0
75	478.5
76	274.5
77	119.0
78	25.0
79	10.0
80	6.0
81	5.5
82	4.5
83	5.5
84	7.0
85	3.0
86	1.0
87	1.5
88	1.5
89	1.5
90	0.5
91	0.0
92	0.0
93	1.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.242144646247597E-4
3	6.032626775443664E-4
4	6.573482969104131E-4
5	6.157439743211464E-4
6	5.242144646247597E-4
7	6.781504582050464E-4
8	6.573482969104131E-4
9	9.152950969638663E-4
10-14	7.688478814496477E-4
15-19	5.841246891533038E-4
20-24	5.774679975390211E-4
25-29	6.939601007889678E-4
30-34	3.9274480524267716E-4
35-39	3.503083962016252E-4
40-44	1.5560016648385726E-4
45-49	1.9137988391062662E-4
50-54	2.2799168778918122E-4
55-59	3.0121529554629053E-4
60-64	2.5129010843917055E-4
65-69	1.7640233071413038E-4
70-74	8.986535100020677E-5
75-79	1.8222699965757966E-4
80-84	4.9925249420305455E-5
85-89	2.1301479278732245E-4
90-94	7.322413540061265E-5
95-99	8.154549006842499E-5
100-104	6.989661300734196E-5
105-109	1.214879022804569E-4
110-114	6.656934228824125E-5
115-119	1.2648312068608305E-4
120-124	1.7142000470789232E-4
125-129	9.652922513827395E-5
130-134	9.486690331576305E-5
135-139	2.454993989134463E-4
140-144	0.02902326076963208
145-149	9.796034320406242E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	1.0
70-71	2.0
72-73	1.0
74-75	0.0
76-77	7.0
78-79	8.0
80-81	10.0
82-83	11.0
84-85	12.0
86-87	40.0
88-89	34.0
90-91	48.0
92-93	39.0
94-95	64.0
96-97	47.0
98-99	66.0
100-101	77.0
102-103	70.0
104-105	67.0
106-107	88.0
108-109	92.0
110-111	96.0
112-113	83.0
114-115	110.0
116-117	124.0
118-119	121.0
120-121	105.0
122-123	131.0
124-125	158.0
126-127	162.0
128-129	179.0
130-131	220.0
132-133	297.0
134-135	362.0
136-137	616.0
138-139	1545.0
140-141	3514.0
142-143	5498.0
144-145	47718.0
146-147	612389.0
148-149	979952.0
150-151	2.2381799E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.43176132574784
#Duplication Level	Percentage of total
1	35.6953429437494
2	11.177167587467245
3	6.505346016792636
4	4.448172049727282
5	3.297266531046498
6	2.6243625452271444
7	2.13158701360625
8	1.8278433489142292
9	1.5323669427336366
>10	17.587603803354934
>50	4.765423341300827
>100	6.554416149263161
>500	1.1442341725945302
>1k	0.6806135736135277
>5k	0.028253980608439325
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	4.160432258926665E-6	1.3729426454457994E-4	1.2065253550887328E-4
2	0.0	0.0	0.0	4.160432258926665E-6	0.001464472155142186	3.078719871605732E-4
3	0.0	0.0	0.0	4.160432258926665E-6	0.0026709975102309188	3.910806323391065E-4
4	1.2481296776779995E-5	0.0	0.0	4.160432258926665E-6	0.0038941645943553586	4.534871162230065E-4
5	1.2481296776779995E-5	0.0	0.0	4.160432258926665E-6	0.005495931014042124	5.117331678479798E-4
6	1.2481296776779995E-5	0.0	0.0	4.160432258926665E-6	0.006598445562657691	5.574979226961732E-4
7	1.2481296776779995E-5	0.0	0.0	8.32086451785333E-6	0.007634393195130431	5.824605162497331E-4
8	1.2481296776779995E-5	0.0	0.0	8.32086451785333E-6	0.009290245234183243	6.157439743211464E-4
9	1.2481296776779995E-5	0.0	0.0	8.32086451785333E-6	0.012601949312288868	7.280756453121663E-4
10-11	1.2481296776779995E-5	0.0	0.0	8.32086451785333E-6	0.017080654639023425	8.487281808210398E-4
12-13	1.2481296776779995E-5	0.0	0.0	8.32086451785333E-6	0.020579578168780747	9.985037421423996E-4
14-15	1.4561512906243327E-5	0.0	0.0	8.32086451785333E-6	0.024511186653466445	0.0011274771421691263
16-17	2.496259355355999E-5	0.0	0.0	1.8721945165169992E-5	0.02788321699932651	0.0012294077325128295
18-19	2.496259355355999E-5	0.0	0.0	2.2882377424096657E-5	0.030658225316030595	0.0013354987551154594
20-21	2.496259355355999E-5	0.0	0.0	2.7042809683023322E-5	0.03375774734893096	0.0014499106422359428
22-23	2.496259355355999E-5	0.0	0.0	3.120324194194999E-5	0.03651403372046988	0.0015476808003207195
24-25	2.496259355355999E-5	0.0	0.0	3.328345807141332E-5	0.03915174777262938	0.001657932255182276
26-27	2.496259355355999E-5	0.0	0.0	3.328345807141332E-5	0.04236984212490916	0.0017286596035840293
28-29	2.496259355355999E-5	0.0	0.0	3.7443890330339985E-5	0.045583776044930004	0.0018160286810214893
30-31	2.496259355355999E-5	0.0	0.0	4.160432258926665E-5	0.049230394919879225	0.0018805153810348527
32-33	2.496259355355999E-5	0.0	0.0	6.656691614282664E-5	0.052463050785065246	0.002007408564932116
34-35	2.496259355355999E-5	0.0	0.0	8.736907743745996E-5	0.055539690440541516	0.0021405423972177696
36-37	2.496259355355999E-5	0.0	0.0	8.736907743745996E-5	0.058531041234709785	0.0022507938520793256
38-39	2.496259355355999E-5	0.0	0.0	9.152950969638663E-5	0.06140381970949865	0.0023173607682221526
40-41	2.496259355355999E-5	0.0	0.0	9.152950969638663E-5	0.0643618870455955	0.0023860079004944424
42-43	2.9123025812486655E-5	0.0	0.0	9.56899419553133E-5	0.06731163351717451	0.0024712967618024393
44-45	3.7443890330339985E-5	2.0802161294633325E-6	0.0	9.56899419553133E-5	0.07012200550807948	0.002546184542463119
46-47	3.7443890330339985E-5	4.160432258926665E-6	0.0	1.0817123873209329E-4	0.07310919586998882	0.0026169118908648723
48-49	3.7443890330339985E-5	4.160432258926665E-6	0.0	1.1025145486155662E-4	0.076872306848188	0.0026855590231371624
50-51	4.160432258926665E-5	4.160432258926665E-6	0.0	1.1233167099101995E-4	0.07986989829074465	0.0027271633457264287
52-53	4.368453871872998E-5	4.160432258926665E-6	0.0	1.1233167099101995E-4	0.08262826487841304	0.0027916500457397923
54-55	4.5764754848193315E-5	8.32086451785333E-6	0.0	1.1441188712048329E-4	0.0853471073596216	0.002858216961882619
56-57	4.992518710711998E-5	8.32086451785333E-6	0.0	1.1857231937940995E-4	0.08829061318281223	0.0028956608522129586
58-59	5.200540323658331E-5	8.32086451785333E-6	0.0	1.2065253550887328E-4	0.09083471750914587	0.002976789281262029
60-61	5.824605162497331E-5	8.32086451785333E-6	0.0	1.2065253550887328E-4	0.09352443696454196	0.0030662385748289524
62-63	5.824605162497331E-5	8.32086451785333E-6	0.0	1.2065253550887328E-4	0.09614134885540684	0.0031348857071012417
64-65	6.448670001336331E-5	8.32086451785333E-6	0.0	1.2065253550887328E-4	0.09875618053014225	0.0032430569458333355
66-67	7.280756453121664E-5	8.32086451785333E-6	0.0	1.2065253550887328E-4	0.10131900680164108	0.003315864510364552
68-69	7.488778066067997E-5	8.32086451785333E-6	0.0	1.2065253550887328E-4	0.10366965102793463	0.0034053138039314753
70-71	7.488778066067997E-5	8.32086451785333E-6	0.0	1.414546968035066E-4	0.10607646108972371	0.0034573192071680587
72-73	8.736907743745996E-5	1.0401080647316662E-5	0.0	1.414546968035066E-4	0.10845622834182977	0.0035696508781590786
74-75	8.736907743745996E-5	1.2481296776779995E-5	0.0	1.4561512906243327E-4	0.1110128139649402	0.003654939739467075
76-77	8.94492935669233E-5	1.664172903570666E-5	0.0	1.4977556132135994E-4	0.11378782228164429	0.0037215066556099017
78-79	9.56899419553133E-5	2.0802161294633325E-5	0.0	1.4977556132135994E-4	0.11699343533714729	0.003758950545940242
80-81	9.985037421423996E-5	2.2882377424096657E-5	0.0	1.4977556132135994E-4	0.11990781813452542	0.0038317581104714586
82-83	1.0193059034370329E-4	2.496259355355999E-5	0.0	1.539359935802866E-4	0.12250600808022512	0.003865041568542872
84-85	1.0401080647316662E-4	2.496259355355999E-5	0.0	1.539359935802866E-4	0.1251562034291614	0.003917046971779455
86-87	1.0817123873209329E-4	2.496259355355999E-5	0.0	1.539359935802866E-4	0.12815379487171807	0.003985694104051744
88-89	1.0817123873209329E-4	2.496259355355999E-5	0.0	1.539359935802866E-4	0.13092256254003376	0.004066822533100815
90-91	1.1649210324994662E-4	2.7042809683023322E-5	0.0	1.539359935802866E-4	0.13359564026639414	0.004168753123444518
92-93	1.1649210324994662E-4	2.9123025812486655E-5	0.0	1.539359935802866E-4	0.13575282439264763	0.004256122200881979
94-95	1.1649210324994662E-4	2.9123025812486655E-5	0.0	1.539359935802866E-4	0.1376999066898253	0.004328929765413195
96-97	1.268931838972633E-4	2.9123025812486655E-5	0.0	1.539359935802866E-4	0.13975516022573506	0.004374694520261388
98-99	1.2897340002672661E-4	3.328345807141332E-5	0.0	1.539359935802866E-4	0.14162319430999315	0.004451662517051532
100-101	1.3105361615618993E-4	3.7443890330339985E-5	0.0	1.539359935802866E-4	0.14385942664916623	0.0045307107299711385
102-103	1.3313383228565328E-4	3.7443890330339985E-5	0.0	1.539359935802866E-4	0.14566921468179933	0.004572315052560404
104-105	1.3313383228565328E-4	3.7443890330339985E-5	0.0	1.539359935802866E-4	0.14730842499181643	0.004622240239667525
106-107	1.3313383228565328E-4	4.160432258926665E-5	0.0	1.664172903570666E-4	0.14910989215993167	0.004657603913868401
108-109	1.3313383228565328E-4	4.160432258926665E-5	0.0	1.851392355222366E-4	0.15134612449910476	0.004695047804198741
110-111	1.3729426454457994E-4	4.160432258926665E-5	0.0	1.913798839106266E-4	0.1537945388834831	0.004772015800988885
112-113	1.3729426454457994E-4	4.160432258926665E-5	0.0	1.913798839106266E-4	0.15560224669998673	0.004840662933261175
114-115	1.3729426454457994E-4	4.160432258926665E-5	0.0	1.9554031616955325E-4	0.15711456382610656	0.004917630930051318
116-117	1.476953451918966E-4	4.160432258926665E-5	0.0	1.9762053229901657E-4	0.15881826083613704	0.0049425935236048785
118-119	1.4977556132135994E-4	4.160432258926665E-5	0.0	2.0178096455794327E-4	0.16050323590100235	0.004973796765546828
120-121	1.5185577745082329E-4	4.160432258926665E-5	0.0	2.0386118068740658E-4	0.1623213447981533	0.005015401088136095
122-123	1.5809642583921327E-4	4.160432258926665E-5	0.0	2.059413968168699E-4	0.16466158794379954	0.005038283465560192
124-125	1.6225685809813993E-4	4.5764754848193315E-5	0.0	2.0802161294633325E-4	0.1664276914377139	0.005077807572019994
126-127	1.6225685809813993E-4	4.5764754848193315E-5	0.0	2.2050290972311324E-4	0.1680356985057891	0.005111091030091408
128-129	1.7057772261599326E-4	4.5764754848193315E-5	0.0	2.3298420649989324E-4	0.16997654015457836	0.005150615136551212
130-131	1.809788032633099E-4	4.784497097765665E-5	0.0	2.371446387588199E-4	0.17174680408075166	0.0051755777301047716
132-133	1.8929966778116324E-4	4.992518710711998E-5	0.0	2.4130507101774657E-4	0.1731343082391037	0.005198460107528868
134-135	1.9554031616955325E-4	4.992518710711998E-5	0.0	2.4338528714720991E-4	0.17482136352009847	0.005231743565600281
136-137	1.9970074842847992E-4	4.992518710711998E-5	2.0802161294633325E-6	2.496259355355999E-4	0.17710752104637867	0.005265027023671695
138-139	1.9970074842847992E-4	4.992518710711998E-5	4.160432258926665E-6	2.5794680005345323E-4	0.1787238489789717	0.0052879094010957915
>>END_MODULE
