##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.1uM.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24035964
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	54-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.25855547129294	37.0	35.0	38.0	33.0	39.0
2	35.44918160969121	35.0	34.0	37.0	33.0	39.0
3	35.52653007801143	36.0	35.0	37.0	33.0	39.0
4	35.66355245830789	36.0	35.0	38.0	33.0	39.0
5	35.55793535054388	36.0	35.0	38.0	33.0	39.0
6	35.52388658095843	36.0	35.0	37.0	33.0	39.0
7	35.435003730243565	35.0	34.0	37.0	33.0	39.0
8	35.60991108989845	36.0	35.0	38.0	33.0	39.0
9	35.597141516770456	36.0	35.0	38.0	33.0	39.0
10-14	35.56720244713297	36.0	35.0	37.8	33.0	39.0
15-19	35.51663448988357	36.0	35.0	38.0	33.0	39.0
20-24	35.48484113223002	36.0	35.0	38.0	33.0	39.0
25-29	35.439399243566854	36.0	35.0	38.0	33.0	39.0
30-34	35.40673128816469	36.0	34.0	38.0	32.8	39.0
35-39	35.372134581329874	36.0	34.0	38.0	32.0	39.0
40-44	35.331460955757805	36.0	34.0	38.0	32.0	39.0
45-49	35.28434971861333	36.0	34.0	38.0	32.0	39.0
50-54	35.23767494409627	36.0	34.0	38.0	32.0	39.0
55-59	35.19629256173689	36.0	34.0	38.0	32.0	39.0
60-64	35.14529457692119	36.0	34.0	38.0	32.0	39.0
65-69	35.090810356219414	36.0	34.0	37.6	32.0	39.0
70-74	35.030001297757295	35.6	34.0	37.6	31.4	39.0
75-79	34.968483518887716	35.2	34.0	37.2	31.0	39.0
80-84	34.90369036371352	35.2	34.0	37.2	31.0	39.0
85-89	34.81882004893548	35.0	34.0	37.0	31.0	39.0
90-94	34.74477194882719	35.0	34.0	37.0	30.4	39.0
95-99	34.68410909613015	35.0	34.0	37.0	30.0	39.0
100-104	34.62312722556156	35.0	34.0	37.0	30.0	39.0
105-109	34.50811181202788	35.0	34.0	37.0	30.0	39.0
110-114	34.433811722011704	35.0	34.0	37.0	29.8	39.0
115-119	34.34571974513661	35.0	34.0	37.0	29.0	39.0
120-124	34.280681160604374	35.0	34.0	37.0	29.0	39.0
125-129	34.14552825444123	35.0	34.0	37.0	28.8	39.0
130-134	34.05304296330585	35.0	34.0	37.0	28.0	39.0
135-139	33.945267939353364	35.0	33.2	37.0	28.0	39.0
140-144	33.81025502666786	35.0	33.0	37.0	27.2	39.0
145-149	33.78984319788414	35.0	33.0	37.0	27.2	39.0
150	34.78026971693196	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	48.0
21	597.0
22	4138.0
23	19682.0
24	61626.0
25	134389.0
26	230486.0
27	347914.0
28	465258.0
29	569341.0
30	690520.0
31	854475.0
32	1096256.0
33	1487175.0
34	2210503.0
35	3842113.0
36	1.0524877E7
37	1496564.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.154531933897054	20.851637155056483	15.601171644291028	29.392659266755434
2	23.55271973084665	26.03381025341069	33.81394787469094	16.59952214105172
3	19.608425679736037	29.469513746659793	32.068924429358894	18.853136144245276
4	23.13419450905308	33.77012445422432	24.219898322269604	18.875782714452995
5	24.44052255262529	35.5472049300027	23.771832822985182	16.240439694386826
6	19.445423532836045	38.76390395658772	24.46008822446231	17.330584286113925
7	20.01892247800005	21.4691160296296	39.48114167586538	19.03081981650497
8	21.62536522354585	23.759375742117104	30.650174879609573	23.965084154727474
9	23.018381954640972	23.41491275323927	30.243887867364087	23.32281742475567
10-14	22.733321617556093	29.633519171521478	26.89357164954982	20.739587561372616
15-19	22.62769573128001	28.52050036353857	28.52856660960218	20.32323729557924
20-24	22.62299688998502	28.655172494993273	28.28286369273334	20.43896692228837
25-29	22.699425416370936	28.619295565307628	28.23362934912421	20.447649669197222
30-34	22.725130558524718	28.421914760731042	28.428084681771033	20.424869998973204
35-39	22.820725642624527	28.46189401847997	28.234705294116768	20.482675044778734
40-44	23.054611830838155	28.210286885102676	28.23304694581836	20.502054338240814
45-49	23.048832158344055	28.162716502654106	28.276424444636376	20.51202689436546
50-54	23.111060575727272	28.10985321828573	28.26292717030197	20.516159035685025
55-59	23.2271991174957	27.942230663018762	28.27223617451021	20.558334044975325
60-64	23.09868367651287	27.89583491169752	28.34398092092095	20.66150049086866
65-69	23.120015392944705	27.919051643099824	28.398190663327643	20.562742300627825
70-74	23.34460943190768	27.848663694242404	28.26261342308525	20.544113450764666
75-79	23.23697514890367	27.84716457393773	28.348736372486407	20.567123904672194
80-84	23.355531609888217	27.95363422136175	28.124747121052362	20.566087047697675
85-89	23.377809449518455	27.895428780667153	28.233797357710884	20.492964412103507
90-94	23.346673613527898	27.91578594164615	28.22889313619006	20.508647308635894
95-99	23.280366580412277	28.01727499849362	28.249548640889888	20.452809780204216
100-104	23.47802047307033	27.961153330264615	28.125353413185593	20.43547278347946
105-109	23.23611393196244	28.04336805612337	28.36030927155126	20.360208740362935
110-114	23.33026095113639	27.999708743860356	28.28279130210095	20.387239002902298
115-119	23.436585512482306	28.01791810108692	28.32785033915755	20.21764604727322
120-124	23.487530292061937	28.028004445606186	28.334605639403325	20.149859622928552
125-129	23.416046187343753	28.219806351312528	28.21103710408855	20.15311035725517
130-134	23.572069336596826	28.211054885351466	28.145410815338867	20.07146496271284
135-139	23.38327067544405	28.2267882987234	28.357959988524588	20.031981037307965
140-144	23.36152687090239	28.257371668743183	28.254354519458342	20.126746940896087
145-149	23.218310074760108	28.113006795396117	28.38331086155398	20.2853722682898
150	23.787583460336297	26.98292594648992	28.47536345516678	20.754127138007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	2.0
6	2.5
7	2.0
8	7.5
9	13.0
10	23.0
11	44.0
12	66.5
13	99.5
14	177.0
15	303.5
16	523.5
17	894.0
18	1452.0
19	2329.0
20	3698.0
21	5826.0
22	8698.0
23	12664.0
24	18249.0
25	26796.0
26	43954.0
27	61159.5
28	77225.5
29	104793.5
30	138936.0
31	174971.5
32	223790.0
33	288857.0
34	365271.0
35	459462.5
36	580164.0
37	724108.5
38	870085.5
39	1031752.0
40	1213352.5
41	1377619.5
42	1519387.5
43	1613096.0
44	1641851.0
45	1642241.0
46	1592583.0
47	1465727.0
48	1297093.0
49	1104951.5
50	920203.0
51	757360.0
52	607380.0
53	501092.5
54	415386.5
55	326312.0
56	242537.5
57	170875.5
58	118938.0
59	78872.0
60	55315.0
61	43348.5
62	31817.0
63	21132.5
64	14645.5
65	10195.5
66	6719.5
67	4705.5
68	3757.5
69	2855.5
70	1987.5
71	1452.0
72	1142.5
73	940.0
74	896.5
75	861.0
76	629.5
77	313.0
78	127.5
79	58.0
80	19.0
81	9.0
82	3.5
83	1.5
84	2.0
85	2.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.160432258926665E-6
3	6.656691614282664E-5
4	9.111346647049396E-4
5	0.12942688714294961
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	1.081712387320933E-5
25-29	0.05047852459755723
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
54-55	2.0
56-57	0.0
58-59	0.0
60-61	1.0
62-63	2.0
64-65	7.0
66-67	11.0
68-69	7.0
70-71	17.0
72-73	27.0
74-75	27.0
76-77	44.0
78-79	36.0
80-81	66.0
82-83	89.0
84-85	105.0
86-87	143.0
88-89	171.0
90-91	176.0
92-93	199.0
94-95	272.0
96-97	289.0
98-99	363.0
100-101	419.0
102-103	455.0
104-105	446.0
106-107	497.0
108-109	493.0
110-111	503.0
112-113	582.0
114-115	660.0
116-117	677.0
118-119	791.0
120-121	857.0
122-123	974.0
124-125	1053.0
126-127	1232.0
128-129	1405.0
130-131	1711.0
132-133	2100.0
134-135	2656.0
136-137	3487.0
138-139	6024.0
140-141	9720.0
142-143	14142.0
144-145	67410.0
146-147	746427.0
148-149	1171015.0
150-151	2.1998174E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.91601679184357
#Duplication Level	Percentage of total
1	27.744295513994416
2	10.993115499185944
3	6.846485867752927
4	4.794251539267977
5	3.625378009768465
6	2.9209666394880585
7	2.472642737643362
8	1.94998828240218
9	1.6854550930367231
>10	19.616776398558223
>50	5.670295594233405
>100	8.297687078938493
>500	1.7478287304112112
>1k	1.4853074530378703
>5k	0.09992961049565079
>10k+	0.049595951785240684
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.0802161294633325E-5	0.0	0.0	0.0	0.0016849750648652993	0.0
2	5.824605162497331E-5	0.0	0.0	8.32086451785333E-6	0.012568665854217455	8.32086451785333E-6
3	2.6626766457130656E-4	0.0	0.0	8.32086451785333E-6	0.015010839590207407	8.32086451785333E-6
4	4.3268495492837316E-4	0.0	0.0	1.2481296776779995E-5	0.0183641479909023	8.32086451785333E-6
5	4.8261014203549314E-4	0.0	0.0	1.2481296776779995E-5	0.022462173765945066	8.32086451785333E-6
6	5.574979226961732E-4	0.0	0.0	1.664172903570666E-5	0.024954272689042135	8.32086451785333E-6
7	6.240648388389998E-4	0.0	0.0	1.664172903570666E-5	0.02819524941874601	8.32086451785333E-6
8	6.94792187240753E-4	0.0	0.0	1.664172903570666E-5	0.03403233587802012	8.32086451785333E-6
9	0.0011149958453923463	0.0	0.0	1.664172903570666E-5	0.04478705326734555	8.32086451785333E-6
10-11	0.0017016167939010061	4.160432258926665E-6	0.0	2.0802161294633325E-5	0.05268979434317675	1.4561512906243327E-5
12-13	0.0017473815487491994	4.160432258926665E-6	0.0	3.536367420087665E-5	0.05892004165091943	1.664172903570666E-5
14-15	0.0018721945165169993	4.160432258926665E-6	0.0	4.160432258926665E-5	0.07749429147089752	1.664172903570666E-5
16-17	0.0019408416487892892	4.160432258926665E-6	0.0	4.5764754848193315E-5	0.0941068974807917	1.8721945165169992E-5
18-19	0.002286157526280202	4.160432258926665E-6	0.0	4.992518710711998E-5	0.10015408576914162	2.0802161294633325E-5
20-21	0.0026855590231371624	4.160432258926665E-6	0.0	4.992518710711998E-5	0.10711240872219646	2.0802161294633325E-5
22-23	0.0031265648425833886	4.160432258926665E-6	0.0	5.200540323658331E-5	0.11413105794300574	2.0802161294633325E-5
24-25	0.0031640087329137287	4.160432258926665E-6	0.0	5.616583549550998E-5	0.11927959286342749	2.0802161294633325E-5
26-27	0.003209773487761922	4.160432258926665E-6	0.0	6.032626775443664E-5	0.12377493991919775	2.2882377424096657E-5
28-29	0.0034281961813555717	4.160432258926665E-6	0.0	6.448670001336331E-5	0.1289359561363963	3.7443890330339985E-5
30-31	0.0037693516265875585	4.160432258926665E-6	0.0	6.864713227228997E-5	0.13252432895972052	4.5764754848193315E-5
32-33	0.0038192768136946784	4.160432258926665E-6	0.0	7.07273484017533E-5	0.1367076435960713	4.784497097765665E-5
34-35	0.003979453455663355	4.160432258926665E-6	0.0	7.488778066067997E-5	0.14025233188067682	5.200540323658331E-5
36-37	0.004021057778252622	4.160432258926665E-6	0.0	7.904821291960663E-5	0.14254056962308648	6.448670001336331E-5
38-39	0.004139630097632031	4.160432258926665E-6	0.0	7.904821291960663E-5	0.14473727785579976	7.07273484017533E-5
40-41	0.004226999175069492	4.160432258926665E-6	0.0	9.56899419553133E-5	0.1471274461885531	7.280756453121664E-5
42-43	0.004530710729971138	6.2406483883899975E-6	0.0	1.0401080647316662E-4	0.14935535766320834	8.94492935669233E-5
44-45	0.004984197846194145	8.32086451785333E-6	0.0	1.0401080647316662E-4	0.15198891128310893	1.0401080647316662E-4
46-47	0.005456406907582321	1.0401080647316662E-5	0.0	1.0401080647316662E-4	0.15440404220941586	1.1441188712048329E-4
48-49	0.005687310897952751	1.2481296776779995E-5	0.0	1.1233167099101995E-4	0.15646761660984349	1.3313383228565328E-4
50-51	0.006178241904506098	1.2481296776779995E-5	0.0	1.1649210324994662E-4	0.1585727953328604	1.476953451918966E-4
52-53	0.006525637998126475	1.2481296776779995E-5	0.0	1.3521404841511663E-4	0.16079238594299775	1.851392355222366E-4
54-55	0.006816868256251341	1.4561512906243327E-5	0.0	1.4561512906243327E-4	0.1624752807917336	2.1426226133472323E-4
56-57	0.007006167924032504	1.664172903570666E-5	0.0	1.4561512906243327E-4	0.1640021594307597	2.454655032766732E-4
58-59	0.007083135920822647	1.664172903570666E-5	0.0	1.4561512906243327E-4	0.16538758337298226	2.808291774775499E-4
60-61	0.007289077317639517	1.8721945165169992E-5	0.0	1.476953451918966E-4	0.16682917315070034	3.265939323257432E-4
62-63	0.007985949721009734	2.2882377424096657E-5	0.0	1.4977556132135994E-4	0.1682603618477711	3.806795516917899E-4
64-65	0.009125908159955641	2.496259355355999E-5	0.0	1.6017664196867662E-4	0.1694752080673777	4.264443065399832E-4
66-67	0.009851903589138343	2.7042809683023322E-5	0.0	1.664172903570666E-4	0.17068381363859592	4.680486291292498E-4
68-69	0.010507171669919293	3.328345807141332E-5	0.0	1.913798839106266E-4	0.1718799379130373	5.075727355890532E-4
70-71	0.010916974247423569	3.328345807141332E-5	0.0	1.9554031616955325E-4	0.17307398197134927	5.783000839908064E-4
72-73	0.012054852470240011	3.328345807141332E-5	0.0	1.9762053229901657E-4	0.1740579242005854	6.490274323925598E-4
74-75	0.012743404009092376	3.328345807141332E-5	0.0	1.9970074842847992E-4	0.175075149887893	6.739900259461198E-4
76-77	0.013217693286610016	3.328345807141332E-5	0.0	2.0178096455794327E-4	0.17619638638167373	7.093537001469964E-4
78-79	0.013419474251167959	3.328345807141332E-5	0.0	2.0386118068740658E-4	0.17742579411418657	7.57198671124653E-4
80-81	0.013735667102846384	3.328345807141332E-5	0.0	2.0802161294633325E-4	0.17847630325956554	7.75920616289823E-4
82-83	0.013924966770627548	3.328345807141332E-5	0.0	2.0802161294633325E-4	0.17942696203073027	8.279260195264064E-4
84-85	0.014274443080377388	3.328345807141332E-5	0.0	2.0802161294633325E-4	0.18039426253093074	8.63289693727283E-4
86-87	0.014397175832015725	3.328345807141332E-5	0.0	2.1218204520525991E-4	0.181315798276283	9.06974232446013E-4
88-89	0.014436699938475527	3.328345807141332E-5	0.0	2.1634247746418658E-4	0.18232054266681377	9.527389872942063E-4
90-91	0.014447101019122846	3.328345807141332E-5	0.0	2.2882377424096657E-4	0.1833190464089562	0.0010130652550486429
92-93	0.014457502099770161	3.328345807141332E-5	0.0	2.4338528714720991E-4	0.18436955555433515	0.0010796321711914695
94-95	0.014461662532029088	3.328345807141332E-5	0.0	2.4546550327667326E-4	0.18532437475775884	0.0011253969260396628
96-97	0.014478304261064796	4.368453871872998E-5	0.0	2.475457194061366E-4	0.18644353103541011	0.0011607606002405394
98-99	0.014509507503006745	4.5764754848193315E-5	0.0	2.5794680005345323E-4	0.18760013120339172	0.0012086055712181962
100-101	0.014542790961078158	4.784497097765665E-5	0.0	2.621072323123799E-4	0.18873800942620816	0.0012710120551020962
102-103	0.014555272257854936	4.992518710711998E-5	0.0	2.6418744844184327E-4	0.18972611208770324	0.0012938944325261927
104-105	0.014573994203020108	4.992518710711998E-5	0.0	2.7666874521862323E-4	0.19055819853948858	0.0013542207002806294
106-107	0.014586475499796888	4.992518710711998E-5	0.0	2.829093936070132E-4	0.19147349363645244	0.0013958250228698961
108-109	0.014609357877220983	4.992518710711998E-5	0.0	2.870698258659399E-4	0.19249487975601892	0.0014540710744948694
110-111	0.014655122632069178	4.992518710711998E-5	0.0	3.0787198716057326E-4	0.19359739430463452	0.001520637990637696
112-113	0.014711288467564688	4.992518710711998E-5	0.0	3.120324194194999E-4	0.19453557177902248	0.0015788840422626695
114-115	0.014727930196600394	4.992518710711998E-5	0.0	3.1619285167842654E-4	0.195355176934031	0.0016288092293697894
116-117	0.014738331277247711	4.992518710711998E-5	0.0	3.224335000668165E-4	0.19642024759231624	0.0016433707422760326
118-119	0.0147445719256361	4.992518710711998E-5	0.0	3.2451371619627987E-4	0.19740210960542293	0.0017182585229367127
120-121	0.014759133438542344	4.992518710711998E-5	0.0	3.3491479684359654E-4	0.19830700362173947	0.0017536221971375895
122-123	0.014765374086930735	5.4085619366046645E-5	0.0	3.432356613614499E-4	0.1993907962251899	0.0018430714907045126
124-125	0.014771614735319124	5.4085619366046645E-5	0.0	3.515565258793032E-4	0.20036433737377873	0.0018909164616821694
126-127	0.014779935599836976	5.824605162497331E-5	0.0	3.619576065266199E-4	0.20136700154818005	0.001963724026213386
128-129	0.014786176248225367	5.824605162497331E-5	0.0	3.723586871739365E-4	0.20257560711939826	0.0020115689971910425
130-131	0.014786176248225367	5.824605162497331E-5	0.0	3.7443890330339985E-4	0.20359075259057635	0.002057333752039236
132-133	0.014786176248225367	5.824605162497331E-5	0.0	3.7443890330339985E-4	0.20468702649080353	0.002109339155275819
134-135	0.014786176248225367	5.824605162497331E-5	0.0	3.7443890330339985E-4	0.2056252039651915	0.002175906071418646
136-137	0.014786176248225367	5.824605162497331E-5	0.0	3.7859933556232654E-4	0.20680052607833826	0.0022175103940079125
138-139	0.014786176248225367	6.032626775443664E-5	0.0	3.806795516917899E-4	0.20774910463337357	0.0022611949327266427
>>END_MODULE
