##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.1uM.2.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24119014
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	72-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.02604600668999	37.0	35.0	38.0	33.0	39.0
2	35.20464248662901	35.0	34.0	38.0	32.0	39.0
3	35.410809040535405	36.0	34.0	38.0	32.0	39.0
4	35.39875717970892	36.0	35.0	38.0	32.0	39.0
5	35.31410011205267	36.0	35.0	38.0	32.0	39.0
6	35.22435502545834	36.0	34.0	37.0	33.0	39.0
7	35.086077938343585	35.0	34.0	37.0	33.0	39.0
8	35.26182790888549	35.0	34.0	37.0	33.0	39.0
9	35.22753973276022	35.0	34.0	37.0	32.0	39.0
10-14	35.262453448553075	35.4	34.2	37.4	32.2	39.0
15-19	35.23755296132752	36.0	34.0	37.0	32.0	39.0
20-24	35.24244685126847	36.0	34.0	37.0	32.0	39.0
25-29	35.228668949734015	36.0	34.0	37.0	32.0	39.0
30-34	35.205085017157	36.0	34.0	37.0	32.0	39.0
35-39	35.170614752327765	36.0	34.0	37.0	32.0	39.0
40-44	35.1512989046733	36.0	34.0	37.0	32.0	39.0
45-49	35.133808463314466	35.8	34.0	37.0	32.0	39.0
50-54	35.107506832576156	35.6	34.0	37.0	32.0	39.0
55-59	35.081606105456885	35.2	34.0	37.0	32.0	39.0
60-64	35.05827778034376	35.0	34.0	37.0	32.0	39.0
65-69	35.02143119117556	35.0	34.0	37.0	32.0	39.0
70-74	35.00831519873268	35.0	34.0	37.0	32.0	39.0
75-79	34.97406519056413	35.0	34.0	37.0	32.0	39.0
80-84	34.949630609237474	35.0	34.0	37.0	32.0	39.0
85-89	34.9151432842735	35.0	34.0	37.0	31.4	39.0
90-94	34.88331302284311	35.0	34.0	37.0	31.0	39.0
95-99	34.838243511334284	35.0	34.0	37.0	31.0	39.0
100-104	34.80957824405162	35.0	34.0	37.0	31.0	39.0
105-109	34.78274568921535	35.0	34.0	37.0	31.0	39.0
110-114	34.73175174113844	35.0	34.0	37.0	31.0	39.0
115-119	34.70325776626108	35.0	34.0	37.0	31.0	39.0
120-124	34.66320310898031	35.0	34.0	37.0	31.0	39.0
125-129	34.61531870627609	35.0	34.0	37.0	30.2	39.0
130-134	34.57296321402447	35.0	34.0	37.0	30.0	39.0
135-139	34.52465178984097	35.0	34.0	37.0	30.0	39.0
140-144	34.48415471951242	35.0	34.0	37.0	30.0	39.0
145-149	34.4775156513964	35.0	34.0	37.0	30.0	39.0
150	35.17735271789035	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	39.0
23	803.0
24	6787.0
25	33244.0
26	103471.0
27	229169.0
28	382319.0
29	529288.0
30	689487.0
31	908038.0
32	1229778.0
33	1734666.0
34	2625594.0
35	4570111.0
36	1.0006398E7
37	1069806.0
38	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.794506359173724	11.93248198288703	15.369649853845601	36.90336180409365
2	22.307381443569106	13.152426317763686	34.330182011406585	30.210010227260618
3	21.18108875177242	17.692835112945566	27.400026145545773	33.72604998973623
4	24.358542735402775	25.299977212826892	22.058282092598848	28.283197959171492
5	24.206393316628	31.78892121815296	24.10189825440632	19.902787210812726
6	18.635847141403747	37.967145719040026	25.30042249913408	18.09658464042215
7	14.936512501864208	26.334861916883824	40.11381474642046	18.614810834831506
8	17.48375181315689	26.218921345528777	31.719309112853455	24.57801772846088
9	18.17660270879238	24.769142666846662	32.75168590533058	24.302568719030372
10-14	19.178069503450835	30.784007810137155	27.322554022383244	22.715368664028766
15-19	19.282991969052716	29.175937769897835	28.374611782874343	23.1664584781751
20-24	19.364992991251967	29.31379645492505	28.029624553288347	23.291586000534636
25-29	19.500690012781135	29.403457015321216	27.86704409019267	23.22880888170498
30-34	19.391187540414833	29.328263434193563	27.853637376805164	23.42691164858644
35-39	19.478539001568386	29.145709916909297	28.00569202937521	23.370059052147102
40-44	19.47152123159578	29.217724246562614	27.943321181464302	23.367433340377303
45-49	19.582700923109893	29.042979621078512	27.946283544062755	23.428035911748836
50-54	19.565108348435633	29.115099374995303	27.940776504467568	23.3790157721015
55-59	19.656186660016736	29.04695701526416	27.902323334353746	23.39453299036536
60-64	19.66426232718512	28.961812280119688	27.833515501119958	23.540409891575237
65-69	19.66348373851462	28.850000579626855	28.0109952644237	23.475520417434826
70-74	19.73986039762646	28.855236141290536	27.96188689208779	23.443016568995212
75-79	19.62848284839463	28.85861149823604	27.969513925315376	23.543391728053955
80-84	19.829264318277932	28.727888137357667	27.847595817687935	23.59525172667647
85-89	19.7973096062525	28.782954859778105	27.972337238324542	23.44739829564486
90-94	19.935560724315373	28.66850387566462	27.885766105330728	23.51016929468928
95-99	19.973699269803767	28.678592505854994	27.924757373131133	23.422950851210107
100-104	20.00741552950161	28.563915452647983	27.918466026748245	23.51020299110216
105-109	19.931021231452807	28.55395357477152	27.967853625711097	23.54717156806458
110-114	20.00442935346656	28.571726390475877	27.894433515718752	23.52941074033881
115-119	20.08550062276965	28.50863645866572	27.95853895476217	23.447323963802464
120-124	20.099592890415853	28.35163553896462	28.061239133188504	23.487532437431028
125-129	20.195124525635915	28.371417605471088	27.978330252023753	23.455127616869245
130-134	20.112860244336474	28.495310107634904	27.937910329351855	23.453919318676768
135-139	20.18156736277839	28.33425049854427	27.95812070551012	23.526061433167218
140-144	20.265672441959293	28.252118714543233	28.037320241347945	23.444888602149526
145-149	19.95668130404616	28.26280635061112	28.24455111223091	23.53596123311181
150	20.358372541805775	27.14601405744782	28.26698432915393	24.228629071592476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.5
7	3.0
8	7.5
9	12.5
10	17.0
11	29.0
12	48.5
13	112.5
14	220.5
15	356.5
16	694.0
17	1321.5
18	2733.5
19	4928.5
20	6166.0
21	8171.5
22	12365.0
23	18072.5
24	26548.0
25	38432.5
26	52887.5
27	72556.5
28	100788.0
29	137850.5
30	182700.5
31	234820.0
32	290202.5
33	349153.0
34	419274.5
35	504977.0
36	603219.0
37	713170.0
38	837218.5
39	977280.0
40	1124062.0
41	1268328.0
42	1402001.0
43	1509655.5
44	1587338.5
45	1617741.0
46	1572117.0
47	1474748.5
48	1333375.5
49	1160278.0
50	980307.5
51	802606.0
52	644581.0
53	516868.5
54	418581.0
55	325939.5
56	242522.0
57	171953.5
58	114658.0
59	74673.5
60	51177.0
61	38244.5
62	28811.0
63	20017.5
64	12885.0
65	8697.5
66	5794.5
67	3770.5
68	2292.0
69	1513.5
70	1376.0
71	1541.5
72	1609.0
73	1156.0
74	677.5
75	427.5
76	217.0
77	87.0
78	26.5
79	10.5
80	8.0
81	4.5
82	3.5
83	5.5
84	6.5
85	4.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.846010122967714E-4
3	7.587374840447458E-4
4	7.545913775745559E-4
5	7.089842064024673E-4
6	6.136237575881004E-4
7	7.089842064024673E-4
8	7.006919934620876E-4
9	9.204356363821506E-4
10-14	8.532687115650748E-4
15-19	6.865952314634421E-4
20-24	6.459633880555814E-4
25-29	7.686881395732015E-4
30-34	3.557359351422906E-4
35-39	3.9056322949188553E-4
40-44	1.4760139033875928E-4
45-49	1.815994633943162E-4
50-54	2.4627872432927815E-4
55-59	2.744722483265692E-4
60-64	1.6833192268970863E-4
65-69	2.0232999574526555E-4
70-74	1.044818856479428E-4
75-79	1.384800020365682E-4
80-84	5.472868573298539E-5
85-89	1.525772482230576E-4
90-94	7.794733229774498E-5
95-99	6.633846265090653E-5
100-104	7.048504499313339E-5
105-109	1.2272783815734035E-4
110-114	5.887668232449189E-5
115-119	1.4594943828829436E-4
120-124	1.9073183431656286E-4
125-129	9.205026923252507E-5
130-134	1.1278481659617299E-4
135-139	1.3103527754963632E-4
140-144	1.5844118428895557E-4
145-149	8.753080358369626E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
72-73	2.0
74-75	4.0
76-77	5.0
78-79	10.0
80-81	12.0
82-83	18.0
84-85	16.0
86-87	32.0
88-89	27.0
90-91	34.0
92-93	47.0
94-95	35.0
96-97	65.0
98-99	54.0
100-101	60.0
102-103	74.0
104-105	78.0
106-107	67.0
108-109	78.0
110-111	85.0
112-113	97.0
114-115	110.0
116-117	118.0
118-119	137.0
120-121	119.0
122-123	116.0
124-125	166.0
126-127	174.0
128-129	210.0
130-131	224.0
132-133	303.0
134-135	418.0
136-137	656.0
138-139	1456.0
140-141	3551.0
142-143	5762.0
144-145	48341.0
146-147	616517.0
148-149	1010060.0
150-151	2.2429676E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.81219492369335
#Duplication Level	Percentage of total
1	37.22513813073439
2	11.087059627480125
3	6.44402810896769
4	4.296605028530929
5	3.340679302500299
6	2.6358294891412672
7	2.064449462498935
8	1.6949248878783862
9	1.533433157833881
>10	17.14462400793801
>50	4.602348069755404
>100	6.24415685712205
>500	1.0733640547277825
>1k	0.5898589360085796
>5k	0.023500878882137075
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	4.1461064701898675E-6	1.2023708763550616E-4	1.285293005758859E-4
2	8.292212940379735E-6	0.0	0.0	2.4876638821139207E-5	0.0016045432039634789	3.109579852642401E-4
3	1.2438319410569603E-5	0.0	0.0	2.4876638821139207E-5	0.002885690103252148	3.8973400819784754E-4
4	2.0730532350949337E-5	0.0	0.0	2.4876638821139207E-5	0.004602178181910753	4.933866699525942E-4
5	2.9022745291329073E-5	0.0	0.0	2.4876638821139207E-5	0.006206721385874232	5.348477346544929E-4
6	2.9022745291329073E-5	0.0	0.0	2.4876638821139207E-5	0.007268124642242838	5.597243734756321E-4
7	3.7314958231708806E-5	0.0	0.0	2.4876638821139207E-5	0.008337820111551824	5.887471187669613E-4
8	3.7314958231708806E-5	0.0	0.0	2.4876638821139207E-5	0.010157960851965176	6.302081834688598E-4
9	4.146106470189867E-5	0.0	0.0	2.4876638821139207E-5	0.013358755046951753	7.089842064024673E-4
10-11	4.5607171172088546E-5	0.0	0.0	2.4876638821139207E-5	0.01776813927799868	7.877602293360748E-4
12-13	4.5607171172088546E-5	4.1461064701898675E-6	0.0	2.4876638821139207E-5	0.021178311849729844	9.639697543191443E-4
14-15	4.768022440718348E-5	4.1461064701898675E-6	0.0	2.694969205623414E-5	0.02521454649845968	0.0011194487469512642
16-17	5.389938411246828E-5	4.1461064701898675E-6	0.0	3.938801146680374E-5	0.028550089153727427	0.0012168822490007261
18-19	5.389938411246828E-5	4.1461064701898675E-6	0.0	4.5607171172088546E-5	0.031475167268446375	0.0013329732301660424
20-21	6.011854381775308E-5	4.1461064701898675E-6	0.0	4.5607171172088546E-5	0.03444170644786723	0.0013930917739837954
22-23	6.633770352303788E-5	4.1461064701898675E-6	0.0	4.5607171172088546E-5	0.03737507677552656	0.0014843061163279728
24-25	6.633770352303788E-5	4.1461064701898675E-6	0.0	4.5607171172088546E-5	0.04021930581407681	0.001633565949254808
26-27	7.462991646341761E-5	4.1461064701898675E-6	0.0	4.5607171172088546E-5	0.04399848186165488	0.0017538030368903141
28-29	7.877602293360748E-5	4.1461064701898675E-6	0.0	4.975327764227841E-5	0.047644982502186864	0.0018636748583503455
30-31	8.292212940379735E-5	4.1461064701898675E-6	0.0	5.597243734756321E-5	0.05133087115418565	0.0019548892006945227
32-33	8.292212940379735E-5	4.1461064701898675E-6	0.0	7.462991646341763E-5	0.054508861763586186	0.0020751262883300287
34-35	8.292212940379735E-5	4.1461064701898675E-6	0.0	8.706823587398723E-5	0.05760600329681802	0.0022036555889059146
36-37	8.292212940379735E-5	4.1461064701898675E-6	0.0	8.706823587398723E-5	0.06064924544593739	0.002292796878014997
38-39	9.536044881436696E-5	4.1461064701898675E-6	0.0	9.121434234417709E-5	0.06364480737064956	0.002375719007418794
40-41	9.536044881436696E-5	4.1461064701898675E-6	0.0	9.121434234417709E-5	0.06680414050093425	0.0024938830418192053
42-43	1.0365266175474669E-4	4.1461064701898675E-6	0.0	9.121434234417709E-5	0.06976860662712	0.002599608756809047
44-45	1.0365266175474669E-4	4.1461064701898675E-6	0.0	9.121434234417709E-5	0.07264600451743176	0.0027115536315041734
46-47	1.0365266175474669E-4	4.1461064701898675E-6	0.0	9.950655528455683E-5	0.07556693652568053	0.0027944757609079706
48-49	1.0365266175474669E-4	6.219159705284802E-6	0.0	1.1401792793022135E-4	0.07941659638325183	0.002896055369427623
50-51	1.0365266175474669E-4	8.292212940379735E-6	0.0	1.2023708763550616E-4	0.08243496189355004	0.0029665391794208504
52-53	1.0779876822493656E-4	8.292212940379735E-6	0.0	1.2023708763550616E-4	0.0851506616315244	0.0030225116167684135
54-55	1.098718214600315E-4	8.292212940379735E-6	0.0	1.2231014087060109E-4	0.08802805952183618	0.0030950684799967362
56-57	1.1194487469512643E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.09094069931714456	0.003144821757639015
58-59	1.1194487469512643E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.09331849137779843	0.0032360360999831917
60-61	1.1401792793022135E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.09579786304697199	0.003341761814973033
62-63	1.1816403440041123E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.09842856760230745	0.0034661450090787294
64-65	1.2023708763550616E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.10129560022644375	0.0035428479787772416
66-67	1.2231014087060109E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.1041004412535272	0.003611258735535375
68-69	1.285293005758859E-4	8.292212940379735E-6	0.0	1.4925983292683523E-4	0.10662956620034302	0.003661012013177653
70-71	1.285293005758859E-4	8.292212940379735E-6	0.0	1.534059393970251E-4	0.10916283725362902	0.0037294227699357857
72-73	1.3267540704607576E-4	1.2438319410569603E-5	0.0	1.596250991023099E-4	0.11152819099487235	0.0038123448993395833
74-75	1.3889456675136055E-4	1.2438319410569603E-5	0.0	1.6169815233740484E-4	0.1141257266984463	0.0038994131352135704
76-77	1.4925983292683525E-4	1.658442588075947E-5	0.0	1.6169815233740484E-4	0.11697410184346674	0.003980262211382273
78-79	1.5755204586721496E-4	1.658442588075947E-5	0.0	1.6169815233740484E-4	0.11982040393525209	0.0040196502228490765
80-81	1.596250991023099E-4	2.0730532350949337E-5	0.0	1.6169815233740484E-4	0.12253403061999135	0.004065257394021166
82-83	1.6999036527778457E-4	2.0730532350949337E-5	0.0	1.6169815233740484E-4	0.12524765730473061	0.004112937618428349
84-85	1.782825782181643E-4	2.0730532350949337E-5	0.0	1.6169815233740484E-4	0.12805664443828424	0.004179275321951387
86-87	1.8242868468835419E-4	2.0730532350949337E-5	0.0	1.6169815233740484E-4	0.1311454937585757	0.004266343557825373
88-89	1.8864784439363898E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.13378656358008667	0.004330608208113316
90-91	1.9486700409892377E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.13654787048923311	0.004388653698695975
92-93	2.0523227027439845E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.13868518837461596	0.004452918348983918
94-95	2.1974364292006297E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.14066495421413167	0.004517182999271861
96-97	2.2388974939025285E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.1425970398292401	0.004573155436619424
98-99	2.2388974939025285E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.1444482763681799	0.004604251235145847
100-101	2.2803585586044273E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.14651718349680465	0.004635347033672271
102-103	2.3218196233063259E-4	2.4876638821139207E-5	0.0	1.6169815233740484E-4	0.14846792659102898	0.004674735045139076
104-105	2.3425501556572753E-4	3.1095798526424007E-5	0.0	1.658442588075947E-4	0.15021136436174382	0.004728634429251545
106-107	2.3632806880082247E-4	3.316885176151894E-5	0.0	1.7620952498306937E-4	0.1519734596115745	0.004794972132774582
108-109	2.3632806880082247E-4	3.316885176151894E-5	0.0	1.8864784439363898E-4	0.1542247954248876	0.00484887151688705
110-111	2.3632806880082247E-4	3.316885176151894E-5	0.0	1.9486700409892377E-4	0.15665026770994867	0.004938012805996132
112-113	2.3632806880082247E-4	3.524190499661387E-5	0.0	1.9486700409892377E-4	0.15850150424888843	0.005000204403048981
114-115	2.3632806880082247E-4	3.7314958231708806E-5	0.0	1.969400573340187E-4	0.16004592890903419	0.005037519361280688
116-117	2.5291249468158193E-4	3.7314958231708806E-5	0.0	1.9901311056911365E-4	0.16174997866828222	0.005070688213042208
118-119	2.5291249468158193E-4	4.146106470189867E-5	0.0	2.0315921703930353E-4	0.16343122484194422	0.0051038570648037265
120-121	2.5498554791667687E-4	4.5607171172088546E-5	0.0	2.0730532350949339E-4	0.16513527460119223	0.005141172023035436
122-123	2.570586011517718E-4	4.975327764227841E-5	0.0	2.093783767445883E-4	0.16738661041450534	0.00517641392803205
124-125	2.5913165438686675E-4	4.975327764227841E-5	0.0	2.19743642920063E-4	0.16906163742846203	0.005205436673323379
126-127	2.736430270325312E-4	4.975327764227841E-5	0.0	2.2388974939025285E-4	0.17060398903537266	0.005226167205674329
128-129	2.8608134644310087E-4	4.975327764227841E-5	0.0	2.2803585586044273E-4	0.17260863151370948	0.005255189950965657
130-131	2.8608134644310087E-4	4.975327764227841E-5	0.0	2.2803585586044273E-4	0.17430024295354693	0.005286285749492081
132-133	2.881543996781958E-4	5.8045490582658146E-5	0.0	2.2803585586044273E-4	0.1757099191534115	0.005329819867429074
134-135	2.9022745291329075E-4	6.011854381775308E-5	0.0	2.2803585586044273E-4	0.1773020240379644	0.005369207878895879
136-137	2.9022745291329075E-4	6.426465028794295E-5	0.0	2.3010890909553765E-4	0.17936056590041366	0.005394084517717017
138-139	2.9022745291329075E-4	6.633770352303788E-5	0.0	2.3218196233063259E-4	0.18068524691763932	0.005418961156538157
>>END_MODULE
