##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.1uM.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24119014
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	62-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.234264675993806	37.0	35.0	38.0	33.0	39.0
2	35.463397425782	36.0	34.0	38.0	33.0	39.0
3	35.55804515889414	36.0	35.0	37.0	33.0	39.0
4	35.691932597244644	36.0	35.0	38.0	33.0	39.0
5	35.565059334515084	36.0	35.0	38.0	33.0	39.0
6	35.549474078832574	36.0	35.0	37.0	33.0	39.0
7	35.4543888485657	35.0	34.0	37.0	33.0	39.0
8	35.63723993858124	36.0	35.0	38.0	33.0	39.0
9	35.616969914275934	36.0	35.0	38.0	33.0	39.0
10-14	35.581538615135756	36.0	35.0	37.8	33.0	39.0
15-19	35.530192030238055	36.0	35.0	38.0	33.0	39.0
20-24	35.50073551928781	36.0	35.0	38.0	33.0	39.0
25-29	35.44842278378378	36.0	35.0	38.0	33.0	39.0
30-34	35.422816620944786	36.0	34.6	38.0	32.8	39.0
35-39	35.3857984990597	36.0	34.0	38.0	32.2	39.0
40-44	35.3435498233883	36.0	34.0	38.0	32.0	39.0
45-49	35.299876802592344	36.0	34.0	38.0	32.0	39.0
50-54	35.25310876306967	36.0	34.0	38.0	32.0	39.0
55-59	35.21304462943635	36.0	34.0	38.0	32.0	39.0
60-64	35.156501079746725	36.0	34.0	38.0	32.0	39.0
65-69	35.097389491567924	36.0	34.0	38.0	32.0	39.0
70-74	35.0333634127541	36.0	34.0	38.0	31.0	39.0
75-79	34.968385327327745	35.2	34.0	37.6	31.0	39.0
80-84	34.90276946357218	35.2	34.0	37.2	31.0	39.0
85-89	34.80726240611252	35.0	34.0	37.0	31.0	39.0
90-94	34.7328994384945	35.0	34.0	37.0	30.0	39.0
95-99	34.66856158039674	35.0	34.0	37.0	30.0	39.0
100-104	34.60934791526683	35.0	34.0	37.0	30.0	39.0
105-109	34.48275967753976	35.0	34.0	37.0	30.0	39.0
110-114	34.410055551119385	35.0	34.0	37.0	29.4	39.0
115-119	34.31646185471705	35.0	34.0	37.0	29.0	39.0
120-124	34.25412781515416	35.0	34.0	37.0	29.0	39.0
125-129	34.1062941609904	35.0	34.0	37.0	28.4	39.0
130-134	34.013156601692984	35.0	33.6	37.0	28.0	39.0
135-139	33.897930296404446	35.0	33.0	37.0	28.0	39.0
140-144	33.75467593304111	35.0	33.0	37.0	27.0	39.0
145-149	33.72944255033823	35.0	33.0	37.0	27.0	39.0
150	34.737386498800106	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	29.0
21	493.0
22	3858.0
23	18580.0
24	57746.0
25	127060.0
26	220573.0
27	339070.0
28	460839.0
29	571912.0
30	698733.0
31	870062.0
32	1122614.0
33	1522115.0
34	2268123.0
35	3957572.0
36	1.0446544E7
37	1433091.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.68078753136426	19.33521411779105	17.576327954368285	28.40767039647641
2	23.70182395108788	25.023383004934736	33.74330450421002	17.531488539767363
3	20.497126102829093	28.53956867432804	31.851257791007924	19.112047431834938
4	23.832034428281105	33.0059742666875	23.934365491847515	19.227625813183884
5	24.82972853709059	35.18092439635973	23.571876671010212	16.417470395539475
6	19.681816180379514	38.790549232236444	24.048528683635244	17.479105903748803
7	20.291103110599796	21.310564353915957	39.23162033074818	19.166712204736065
8	22.12852067667443	23.584239388890442	30.281005682902297	24.006234251532838
9	23.328826792007334	23.21626414744815	29.961490133883583	23.493418926660933
10-14	22.777943575968738	29.497780464823315	26.925277293673776	20.79899866553417
15-19	22.69603807187143	28.339806096551044	28.53507112687111	20.42908470470642
20-24	22.682285282957938	28.527954983762104	28.25210372212421	20.537656011155747
25-29	22.75924253243341	28.53889177857079	28.179579644840047	20.52228604415575
30-34	22.74641575314812	28.36175807186811	28.363359298186904	20.528466876796873
35-39	22.834185510236864	28.400519192036622	28.19105872238393	20.574236575342592
40-44	23.06161935143783	28.153241256048027	28.221671084895927	20.563468307618212
45-49	23.066158508801397	28.102981324195092	28.23709294252244	20.593767224481066
50-54	23.119330857549407	28.09455583959241	28.206606855542326	20.57950644731585
55-59	23.232420695141187	27.906377930706455	28.248607509411457	20.612593864740905
60-64	23.098125118138128	27.875532054317482	28.324468335416547	20.70187449212785
65-69	23.12635195776449	27.91144420464921	28.360717313528227	20.60148652405807
70-74	23.330032309490214	27.812769673492348	28.26972677818204	20.58747123883539
75-79	23.21400823491602	27.82163598751655	28.358711663514548	20.605644114052883
80-84	23.326107001374076	27.909047642088392	28.145818746644984	20.61902660989255
85-89	23.35591715254795	27.863118755581144	28.25548597856347	20.525478113307436
90-94	23.312683498610788	27.87976795783072	28.2572407447872	20.550307798771293
95-99	23.257834784603144	27.982093631586054	28.29001560549101	20.47005597831979
100-104	23.45235996201207	27.923612771281352	28.14392498189186	20.480102284814713
105-109	23.227388936493863	27.986990996933297	28.39091860811735	20.39470145845549
110-114	23.32101568661404	27.957304505113978	28.291069732807262	20.43061007546472
115-119	23.38891012634491	27.952111081280613	28.371337854887624	20.287640937486852
120-124	23.432892074028892	27.96575488959426	28.388850421762513	20.21250261461434
125-129	23.375739366514033	28.14988438055483	28.261392736982533	20.212983515948608
130-134	23.5158308015223	28.129828602084107	28.201924884702663	20.15241571169094
135-139	23.323688748157146	28.13764857932221	28.420809603027624	20.11785306949302
140-144	23.294433731488077	28.205854062296186	28.30090516706002	20.19880703915572
145-149	23.15810633656338	28.129211018690352	28.367108907263994	20.34557373748227
150	23.72379604433896	26.96696741922623	28.473190357585736	20.83604617884907
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	2.0
7	3.0
8	2.5
9	11.0
10	21.0
11	26.0
12	45.5
13	89.5
14	128.0
15	207.5
16	361.5
17	620.5
18	1069.0
19	1733.5
20	2796.0
21	4428.5
22	6717.0
23	10253.0
24	15404.0
25	23203.5
26	39378.5
27	55173.0
28	69935.0
29	96511.0
30	129433.0
31	164344.5
32	213658.0
33	277817.5
34	352275.5
35	446882.5
36	567337.5
37	709771.5
38	858418.0
39	1023888.5
40	1207387.5
41	1376717.0
42	1525545.5
43	1623591.5
44	1657644.0
45	1663990.5
46	1617924.5
47	1492978.0
48	1324619.5
49	1133937.0
50	945670.5
51	776490.5
52	620538.0
53	510482.0
54	424681.5
55	332933.0
56	246311.0
57	172964.5
58	119407.5
59	78615.0
60	54165.0
61	41538.0
62	30455.5
63	20436.0
64	14040.5
65	9732.0
66	6553.0
67	4482.5
68	3395.0
69	2461.5
70	1752.5
71	1339.0
72	1023.0
73	853.0
74	845.5
75	785.0
76	535.0
77	263.0
78	131.0
79	62.0
80	10.0
81	2.5
82	2.5
83	2.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.146106470189868E-6
3	9.536044881436696E-5
4	9.867733399051884E-4
5	0.17056667407714096
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	6.633770352303788E-6
25-29	0.06639989512009073
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.292212940379735E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.29444118247113E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	8.305976561281682E-7
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	3.0
64-65	5.0
66-67	3.0
68-69	10.0
70-71	10.0
72-73	19.0
74-75	27.0
76-77	48.0
78-79	64.0
80-81	91.0
82-83	81.0
84-85	103.0
86-87	139.0
88-89	183.0
90-91	184.0
92-93	236.0
94-95	235.0
96-97	311.0
98-99	361.0
100-101	400.0
102-103	402.0
104-105	519.0
106-107	440.0
108-109	522.0
110-111	522.0
112-113	585.0
114-115	630.0
116-117	684.0
118-119	711.0
120-121	819.0
122-123	959.0
124-125	1145.0
126-127	1172.0
128-129	1419.0
130-131	1720.0
132-133	2119.0
134-135	2571.0
136-137	3445.0
138-139	5977.0
140-141	10012.0
142-143	14656.0
144-145	67297.0
146-147	743080.0
148-149	1210284.0
150-151	2.2044811E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.66115218219329
#Duplication Level	Percentage of total
1	28.52684845068229
2	10.9029308997148
3	6.909485803590111
4	4.9516433708954635
5	3.6089567135007488
6	2.8181360165961915
7	2.312263210948423
8	1.989665240772577
9	1.6344301015281073
>10	19.468900604586207
>50	5.575987057242586
>100	8.229766700572922
>500	1.6051597094890526
>1k	1.404484640473913
>5k	0.06134147940651078
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.658442588075947E-5	0.0	0.0	0.0	0.0016335659492548078	4.1461064701898675E-6
2	4.146106470189867E-5	1.2438319410569603E-5	0.0	8.292212940379735E-6	0.010178691384316124	8.292212940379735E-6
3	2.031592170393035E-4	1.2438319410569603E-5	0.0	8.292212940379735E-6	0.012056877615312135	8.292212940379735E-6
4	3.8973400819784754E-4	1.2438319410569603E-5	0.0	1.2438319410569603E-5	0.014594294775068335	8.292212940379735E-6
5	4.3948728584012594E-4	1.2438319410569603E-5	0.0	1.2438319410569603E-5	0.017633390817717507	8.292212940379735E-6
6	5.01678882892974E-4	1.2438319410569603E-5	0.0	1.658442588075947E-5	0.01968571352046149	8.292212940379735E-6
7	5.763087993563916E-4	1.2438319410569603E-5	0.0	1.658442588075947E-5	0.022098747486111993	8.292212940379735E-6
8	6.467926093496194E-4	1.2438319410569603E-5	0.0	1.658442588075947E-5	0.027098951889160974	8.292212940379735E-6
9	9.909194463753783E-4	1.2438319410569603E-5	0.0	1.658442588075947E-5	0.03711594512113969	8.292212940379735E-6
10-11	0.0014801600098577827	1.658442588075947E-5	0.0	2.4876638821139207E-5	0.044620397832183356	8.292212940379735E-6
12-13	0.0014967444357385422	1.658442588075947E-5	0.0	5.5972437347563206E-5	0.05044775047603521	8.292212940379735E-6
14-15	0.0015485707666159157	1.658442588075947E-5	0.0	7.048380999322775E-5	0.06594796951484003	8.292212940379735E-6
16-17	0.0015796665651423396	1.658442588075947E-5	0.0	7.048380999322775E-5	0.07931916288120236	8.292212940379735E-6
18-19	0.0018698940180556303	1.658442588075947E-5	0.0	7.048380999322775E-5	0.08498689042595191	8.292212940379735E-6
20-21	0.0022824316118395223	1.658442588075947E-5	0.0	7.048380999322775E-5	0.0919461301361656	8.292212940379735E-6
22-23	0.0027032614185637936	1.658442588075947E-5	0.0	7.462991646341761E-5	0.09912511348929935	1.2438319410569603E-5
24-25	0.002753014696206072	1.658442588075947E-5	0.0	7.462991646341761E-5	0.10392423172854412	1.2438319410569603E-5
26-27	0.0028234985061992997	1.658442588075947E-5	0.0	7.462991646341761E-5	0.10841653808899485	1.4511372645664537E-5
28-29	0.00312616427852316	1.658442588075947E-5	0.0	7.462991646341761E-5	0.11364892445437447	1.658442588075947E-5
30-31	0.0034640719558436343	1.8657479115854403E-5	0.0	7.462991646341761E-5	0.11696995573699653	1.658442588075947E-5
32-33	0.0034993138608402485	2.0730532350949337E-5	0.0	7.462991646341761E-5	0.12080717727515727	1.658442588075947E-5
34-35	0.0036527198002372736	2.2803585586044273E-5	0.0	7.462991646341761E-5	0.12425259175188505	1.8657479115854403E-5
36-37	0.003687961705233887	2.4876638821139207E-5	0.0	8.084907616870242E-5	0.12641685932932417	2.2803585586044273E-5
38-39	0.0038081987928693935	2.4876638821139207E-5	0.0	8.706823587398723E-5	0.12844015928677682	2.9022745291329073E-5
40-41	0.003874536496392431	2.4876638821139207E-5	0.0	9.536044881436696E-5	0.13056711190598422	4.5607171172088546E-5
42-43	0.004181348375186482	2.4876638821139207E-5	0.0	1.0779876822493656E-4	0.13264223819431425	6.426465028794295E-5
44-45	0.004662296725728507	2.4876638821139207E-5	0.0	1.1609098116531629E-4	0.13499929972261718	7.255686322832269E-5
46-47	0.005091418745393157	2.4876638821139207E-5	0.0	1.347484602811707E-4	0.13730038881357257	8.914128910908215E-5
48-49	0.00529872406890265	2.4876638821139207E-5	0.0	1.471867796917403E-4	0.1391350409266316	1.1194487469512643E-4
50-51	0.005773453259739391	2.4876638821139207E-5	0.0	1.5547899263212002E-4	0.14119358278908084	1.596250991023099E-4
52-53	0.006132091469410814	2.4876638821139207E-5	0.0	1.6584425880759472E-4	0.14333090067446372	2.0523227027439845E-4
54-55	0.006459633880555813	2.4876638821139207E-5	0.0	1.7413647174797445E-4	0.14495617441077813	2.4876638821139205E-4
56-57	0.006716692481707585	2.4876638821139207E-5	0.0	1.7413647174797445E-4	0.1464923068579835	2.777891335027211E-4
58-59	0.006857660101694041	2.4876638821139207E-5	0.0	1.782825782181643E-4	0.14782735314138462	3.3168851761518944E-4
60-61	0.007098134276965054	2.4876638821139207E-5	0.0	1.8450173792344913E-4	0.1490670389759714	3.814417952574678E-4
62-63	0.007813337643072805	2.4876638821139207E-5	0.0	1.9072089762873392E-4	0.15032952839614422	4.3119507289974624E-4
64-65	0.00876486907798138	2.4876638821139207E-5	4.1461064701898675E-6	1.9279395086382883E-4	0.15133703226840037	4.7265613760164493E-4
66-67	0.009446903592327614	2.4876638821139207E-5	4.1461064701898675E-6	1.9901311056911365E-4	0.15254562230446073	5.099710958333537E-4
68-69	0.010145522532554607	2.4876638821139207E-5	4.1461064701898675E-6	2.1974364292006297E-4	0.15360080640112403	5.410668943597777E-4
70-71	0.0105165990616366	2.4876638821139207E-5	4.1461064701898675E-6	2.218166961551579E-4	0.1546725749236681	6.053315446477207E-4
72-73	0.011517883774187452	2.4876638821139207E-5	4.1461064701898675E-6	2.2388974939025285E-4	0.15553081896299742	6.778884078760433E-4
74-75	0.01209211952030875	2.4876638821139207E-5	4.1461064701898675E-6	2.259628026253478E-4	0.15645954681231994	7.048380999322775E-4
76-77	0.012506730167327736	2.9022745291329073E-5	4.1461064701898675E-6	2.2803585586044273E-4	0.15746290457810588	7.297147387534167E-4
78-79	0.0126850127455459	3.1095798526424007E-5	4.1461064701898675E-6	2.2803585586044273E-4	0.15853260004741487	7.773949631606001E-4
80-81	0.012971094091989	3.316885176151894E-5	4.1461064701898675E-6	2.3840112203591738E-4	0.15948620453555856	8.001985487466445E-4
82-83	0.013141084457266786	3.7314958231708806E-5	4.1461064701898675E-6	2.4876638821139205E-4	0.16026774560518933	8.312943472730685E-4
84-85	0.013393996951948367	3.7314958231708806E-5	4.1461064701898675E-6	2.4876638821139205E-4	0.16116537765598543	8.45805719918733E-4
86-87	0.013522526252524253	3.7314958231708806E-5	4.1461064701898675E-6	2.4876638821139205E-4	0.16206715581325173	8.686093055047773E-4
88-89	0.013537037625169918	3.7314958231708806E-5	4.1461064701898675E-6	2.4876638821139205E-4	0.1630290525143358	9.245817428523405E-4
90-91	0.013551548997815584	3.7314958231708806E-5	4.1461064701898675E-6	2.5913165438686675E-4	0.16401375280100589	9.888463931402834E-4
92-93	0.013580571743106912	3.938801146680374E-5	4.1461064701898675E-6	2.7156997379743634E-4	0.16496321118267937	0.0010924990548950302
94-95	0.013586790902812196	4.146106470189867E-5	4.1461064701898675E-6	2.7571608026762616E-4	0.16591681567082303	0.0011298140131267389
96-97	0.013599229222222765	4.146106470189867E-5	4.1461064701898675E-6	2.7986218673781605E-4	0.16694297702219502	0.0011505445454776883
98-99	0.013611667541633335	4.146106470189867E-5	4.1461064701898675E-6	2.81935239972911E-4	0.1680644988223814	0.0012168822490007261
100-101	0.01362203280780881	4.146106470189867E-5	4.1461064701898675E-6	2.8608134644310087E-4	0.16930418465696814	0.001283219952523764
102-103	0.013626178914278999	4.5607171172088546E-5	4.1461064701898675E-6	3.0473882555895527E-4	0.17019767060129407	0.0013267540704607578
104-105	0.01363861723368957	4.5607171172088546E-5	4.1461064701898675E-6	3.1717714496952486E-4	0.1712093205800204	0.0014013839869241752
106-107	0.013646909446629949	4.5607171172088546E-5	4.1461064701898675E-6	3.254693579099046E-4	0.17210280652434629	0.0014283336789804094
108-109	0.013682151351626563	4.5607171172088546E-5	4.1461064701898675E-6	3.3168851761518944E-4	0.17302738826719866	0.0014905252760332574
110-111	0.013725685469563556	4.5607171172088546E-5	4.1461064701898675E-6	3.5241904996613873E-4	0.17410537594944803	0.001565155192496675
112-113	0.013754708214854885	4.5607171172088546E-5	4.1461064701898675E-6	3.6278431614161343E-4	0.17510873371523397	0.0016335659492548078
114-115	0.013769219587500551	4.5607171172088546E-5	4.1461064701898675E-6	3.710765290819932E-4	0.1760789226292584	0.0016729539607216117
116-117	0.01378165790691112	4.5607171172088546E-5	4.1461064701898675E-6	3.7729568878727796E-4	0.1771050839806304	0.0017123419721884153
118-119	0.013787877066616404	4.5607171172088546E-5	4.1461064701898675E-6	3.7729568878727796E-4	0.17800686213789668	0.0017641683030657886
120-121	0.013800315386026975	4.5607171172088546E-5	4.1461064701898675E-6	3.7729568878727796E-4	0.1789397360936894	0.001811848527472972
122-123	0.013804461492497165	4.5607171172088546E-5	4.1461064701898675E-6	3.8558790172765766E-4	0.18007784231975651	0.001905135923052244
124-125	0.013806534545732259	4.975327764227841E-5	4.1461064701898675E-6	3.938801146680374E-4	0.18117863358759193	0.0019528161474594275
126-127	0.013812753705437544	4.975327764227841E-5	4.1461064701898675E-6	3.980262211382273E-4	0.1821654069274971	0.0020004963718666113
128-129	0.013823118971613018	4.975327764227841E-5	4.1461064701898675E-6	4.0839148731370195E-4	0.1832807095679782	0.002044030489803605
130-131	0.013827265078083208	5.1826330877373346E-5	4.1461064701898675E-6	4.104645405487969E-4	0.18438150083581362	0.002070980181859839
132-133	0.013829338131318304	5.389938411246828E-5	8.292212940379735E-6	4.1461064701898677E-4	0.1854408310389471	0.002124879565972307
134-135	0.013831411184553399	5.389938411246828E-5	8.292212940379735E-6	4.1461064701898677E-4	0.18641931216591193	0.002170486737144396
136-137	0.013831411184553399	5.8045490582658146E-5	8.292212940379735E-6	4.1875675348917665E-4	0.1874993729013964	0.002222313068021769
138-139	0.013831411184553399	6.219159705284801E-5	1.2438319410569603E-5	4.229028599593665E-4	0.18842810075071892	0.0022637741327236676
>>END_MODULE
