##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	T3.1uM.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24141884
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.245603947065604	37.0	35.0	38.0	34.0	39.0
2	35.55187387198116	36.0	35.0	38.0	33.0	39.0
3	35.59420917605271	36.0	35.0	37.0	33.0	39.0
4	35.73869831368587	36.0	35.0	38.0	33.0	39.0
5	35.63598006684151	36.0	35.0	38.0	33.0	39.0
6	35.60303201688816	36.0	35.0	37.0	33.0	39.0
7	35.50673576262731	35.0	34.0	37.0	33.0	39.0
8	35.686657014837785	36.0	35.0	38.0	33.0	39.0
9	35.66324484037783	36.0	35.0	38.0	33.0	39.0
10-14	35.63497776726953	36.0	35.0	37.8	33.0	39.0
15-19	35.58722013576074	36.0	35.0	38.0	33.0	39.0
20-24	35.55483148705379	36.0	35.0	38.0	33.0	39.0
25-29	35.51108939965083	36.0	35.0	38.0	33.0	39.0
30-34	35.47747445062697	36.0	35.0	38.0	33.0	39.0
35-39	35.44295262954623	36.0	34.8	38.0	33.0	39.0
40-44	35.40123105553816	36.0	34.0	38.0	32.6	39.0
45-49	35.354177876092855	36.0	34.0	38.0	32.0	39.0
50-54	35.30761308438066	36.0	34.0	38.0	32.0	39.0
55-59	35.2698780509425	36.0	34.0	38.0	32.0	39.0
60-64	35.210830725151446	36.0	34.0	38.0	32.0	39.0
65-69	35.15499460579302	36.0	34.0	38.0	32.0	39.0
70-74	35.092644722485204	36.0	34.0	38.0	31.8	39.0
75-79	35.025804423771696	36.0	34.0	37.8	31.2	39.0
80-84	34.96039273859691	35.4	34.0	37.4	31.0	39.0
85-89	34.86938921589257	35.0	34.0	37.0	31.0	39.0
90-94	34.79393224752686	35.0	34.0	37.0	31.0	39.0
95-99	34.7272723238472	35.0	34.0	37.0	30.2	39.0
100-104	34.66275572056182	35.0	34.0	37.0	30.0	39.0
105-109	34.54246732962379	35.0	34.0	37.0	30.0	39.0
110-114	34.46274061169494	35.0	34.0	37.0	30.0	39.0
115-119	34.367875658755075	35.0	34.0	37.0	29.0	39.0
120-124	34.296975360564986	35.0	34.0	37.0	29.0	39.0
125-129	34.15242551991565	35.0	34.0	37.0	28.8	39.0
130-134	34.05301712294345	35.0	34.0	37.0	28.0	39.0
135-139	33.93343426298567	35.0	33.0	37.0	28.0	39.0
140-144	33.790669071078376	35.0	33.0	37.0	27.0	39.0
145-149	33.760996240244644	35.0	33.0	37.0	27.2	39.0
150	34.73998211541687	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	18.0
21	380.0
22	2854.0
23	14107.0
24	46685.0
25	105984.0
26	193916.0
27	312250.0
28	433329.0
29	545487.0
30	675486.0
31	851094.0
32	1109584.0
33	1532094.0
34	2322248.0
35	4121173.0
36	1.0528976E7
37	1346219.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.819117016716675	20.744694158914857	17.734026060269365	27.702162764099107
2	23.969070681023513	25.65214155001911	32.72528907542133	17.653498693536044
3	20.567553383395733	28.909500382530435	31.937625378647137	18.585320855426694
4	23.871038266486337	32.717724243883175	24.388492048583377	19.022745441047107
5	25.131110556602266	34.915039861591055	23.699520983993644	16.254328597813036
6	19.976788058463043	38.92615837272683	23.839895842428867	17.257157726381255
7	20.61261250364719	21.782181539767155	38.85863257399464	18.74657338259102
8	22.237502259558532	23.935128675127427	30.12106263123458	23.706306434079462
9	23.423055963652214	23.148533063948115	30.125797141598394	23.302613830801274
10-14	22.830214907834037	29.622901013027814	26.934341164094732	20.612542915043417
15-19	22.81025374821617	28.386838409131617	28.507291311647425	20.295616531004786
20-24	22.772718119640835	28.60112385441509	28.248964888047208	20.377193137896867
25-29	22.88640597354087	28.522200828158002	28.167649320245047	20.42374387805608
30-34	22.82311024276316	28.43073059252542	28.378997264670808	20.36716190004061
35-39	22.941065411464987	28.422458661469836	28.20057125616211	20.435904670903067
40-44	23.143158172742442	28.201179328009363	28.232369934343154	20.423292564905044
45-49	23.1260186653204	28.152141730115183	28.28459286773145	20.437246736832968
50-54	23.18197555072318	28.14738342829734	28.23202719582305	20.43861382515643
55-59	23.310402783809252	27.98819843554877	28.272177101008356	20.42922167963362
60-64	23.17627215447491	27.929218645799608	28.360142771628166	20.53436642809732
65-69	23.200535540790508	27.97075448631642	28.383675151822906	20.44503482107017
70-74	23.41073669093387	27.877795175593672	28.267037835045606	20.444430298426855
75-79	23.26700120956199	27.89326743158349	28.411520484577018	20.428210874277504
80-84	23.412407112970993	27.96096918685549	28.167194402990752	20.459429297182762
85-89	23.410649522814577	27.94402474930609	28.29001196541435	20.35531376246498
90-94	23.384529214054936	27.920137527847405	28.304806271984045	20.390526986113617
95-99	23.314474058489004	28.035497285083295	28.331069666924808	20.318958989502896
100-104	23.520884346612362	27.940673723216094	28.224200725117203	20.31424120505434
105-109	23.290732861717274	28.019377422786828	28.442819728652513	20.247069986843385
110-114	23.38640157909495	28.002849101665284	28.33724228747238	20.273507031767384
115-119	23.431530394890203	27.99009439254551	28.430014002187914	20.14836121037637
120-124	23.47137698195595	27.98042403378534	28.46829508572213	20.079903898536582
125-129	23.424732140559527	28.16020103966687	28.335798301958388	20.079268517815215
130-134	23.562235588189164	28.14471440130138	28.28778110807954	20.00526890242992
135-139	23.35695360487762	28.150814933733848	28.512361478659447	19.97986998272908
140-144	23.31827409196533	28.23548269552041	28.39135675376817	20.05488645874609
145-149	23.214974275004614	28.17134676127821	28.39694012174118	20.216738841975996
150	23.749745425452044	27.01067514052447	28.50833590528474	20.731243528738748
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	2.0
6	4.5
7	3.5
8	10.5
9	23.5
10	35.5
11	48.0
12	78.0
13	118.5
14	190.0
15	311.5
16	504.5
17	851.0
18	1424.0
19	2275.0
20	3470.5
21	5373.0
22	8041.0
23	11727.5
24	16919.0
25	24894.5
26	42256.5
27	58592.5
28	73145.0
29	100224.0
30	133822.5
31	169819.5
32	217798.5
33	282531.5
34	360767.5
35	456922.0
36	578097.5
37	720077.5
38	869358.0
39	1036832.0
40	1219097.5
41	1387050.0
42	1532437.0
43	1629950.0
44	1666656.5
45	1667731.0
46	1611544.5
47	1482464.5
48	1314793.0
49	1122611.0
50	934347.5
51	765126.0
52	609565.5
53	498094.5
54	409444.5
55	319090.0
56	235968.5
57	165932.0
58	115457.5
59	77605.5
60	55073.0
61	42368.5
62	30713.5
63	20856.0
64	14403.5
65	9913.0
66	6649.0
67	4700.5
68	3556.5
69	2705.0
70	2062.0
71	1613.5
72	1303.5
73	1082.5
74	1120.0
75	1081.5
76	717.0
77	349.0
78	184.0
79	85.5
80	23.5
81	6.5
82	4.0
83	3.0
84	3.0
85	2.5
86	1.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.142178795987919E-6
3	5.7990503143830865E-5
4	8.739997259534508E-4
5	0.13445512371776783
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	1.0769664869568589E-5
25-29	0.053289958646143776
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.284357591975839E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.285103124430038E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	3.0
64-65	0.0
66-67	6.0
68-69	6.0
70-71	13.0
72-73	19.0
74-75	23.0
76-77	29.0
78-79	52.0
80-81	61.0
82-83	91.0
84-85	102.0
86-87	129.0
88-89	149.0
90-91	166.0
92-93	193.0
94-95	225.0
96-97	256.0
98-99	290.0
100-101	348.0
102-103	384.0
104-105	396.0
106-107	415.0
108-109	462.0
110-111	479.0
112-113	526.0
114-115	527.0
116-117	594.0
118-119	665.0
120-121	758.0
122-123	847.0
124-125	941.0
126-127	1094.0
128-129	1296.0
130-131	1500.0
132-133	1852.0
134-135	2344.0
136-137	3031.0
138-139	5370.0
140-141	9174.0
142-143	13304.0
144-145	65012.0
146-147	741701.0
148-149	1186452.0
150-151	2.2100599E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.29921813068481
#Duplication Level	Percentage of total
1	28.646292147155272
2	11.434313072898366
3	7.203062676579163
4	5.09967801758981
5	3.8075608720373166
6	3.013421672851423
7	2.4258489876858835
8	2.0119384536305027
9	1.7069603372468962
>10	19.81261450726136
>50	5.357786445653154
>100	7.221299835844275
>500	1.2778347690772696
>1k	0.9330599945537095
>5k	0.04832820993584262
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.284357591975837E-6	0.0	0.0	0.0	0.0015201796181275663	4.142178795987918E-6
2	3.7279609163891266E-5	0.0	0.0	0.0	0.010024072686290764	4.142178795987918E-6
3	2.443885489632872E-4	4.142178795987918E-6	0.0	0.0	0.011937759290037183	4.142178795987918E-6
4	4.2250223719076773E-4	4.142178795987918E-6	0.0	1.2426536387963757E-5	0.014580469361877475	4.142178795987918E-6
5	4.639240251506469E-4	4.142178795987918E-6	0.0	1.2426536387963757E-5	0.01836642078141043	4.142178795987918E-6
6	5.426254222744174E-4	4.142178795987918E-6	0.0	1.2426536387963757E-5	0.020669472191979715	4.142178795987918E-6
7	5.881893890302845E-4	4.142178795987918E-6	0.0	1.6568715183951674E-5	0.023233480866696236	4.142178795987918E-6
8	6.503220709701032E-4	4.142178795987918E-6	0.0	2.0710893979939592E-5	0.028465052686028978	4.142178795987918E-6
9	9.278480503012938E-4	4.142178795987918E-6	0.0	2.0710893979939592E-5	0.03811218710188484	4.142178795987918E-6
10-11	0.0012985730525422125	6.213268193981878E-6	0.0	2.485307277592751E-5	0.044783166052823384	6.213268193981878E-6
12-13	0.001375203360267989	8.284357591975837E-6	0.0	3.313743036790335E-5	0.05008515491168792	8.284357591975837E-6
14-15	0.001474615651371699	8.284357591975837E-6	0.0	3.9350698561885225E-5	0.06399044912981937	8.284357591975837E-6
16-17	0.0015139663499335843	8.284357591975837E-6	0.0	4.1421787959879184E-5	0.07590956861527459	8.284357591975837E-6
18-19	0.0017562838094988776	1.6568715183951674E-5	0.0	4.1421787959879184E-5	0.08100651962373773	8.284357591975837E-6
20-21	0.002083515934381923	1.6568715183951674E-5	0.0	4.1421787959879184E-5	0.08699196798394028	1.0355446989969796E-5
22-23	0.0024666674730108055	1.6568715183951674E-5	0.0	4.763505615386107E-5	0.09293599455618294	1.2426536387963757E-5
24-25	0.0025101603503686785	1.6568715183951674E-5	0.0	5.799050314383086E-5	0.0972335050570204	1.2426536387963757E-5
26-27	0.002555724317124546	1.6568715183951674E-5	0.0	7.041703953179461E-5	0.10105259390692127	1.2426536387963757E-5
28-29	0.0028228948494657667	1.6568715183951674E-5	0.0	7.041703953179461E-5	0.10560691949310999	1.4497625785957714E-5
30-31	0.00317498004712474	1.6568715183951674E-5	0.0	8.077248652176441E-5	0.10866177635515106	2.0710893979939592E-5
32-33	0.0032288283714725827	1.6568715183951674E-5	0.0	8.284357591975837E-5	0.11199830137531934	2.4853072775927514E-5
34-35	0.0033344539307702745	1.6568715183951674E-5	0.0	8.491466531775234E-5	0.11504487387976846	2.6924162173921473E-5
36-37	0.0033945155233120992	1.6568715183951674E-5	0.0	9.527011230772214E-5	0.11719052249609019	2.8995251571915432E-5
38-39	0.0034877145462218276	1.6568715183951674E-5	0.0	9.527011230772214E-5	0.11923468773191023	3.106634096990939E-5
40-41	0.0035498472281616463	1.6568715183951674E-5	0.0	1.0355446989969797E-4	0.12124778662676036	3.9350698561885225E-5
42-43	0.0038522262802687646	1.6568715183951674E-5	0.0	1.0355446989969797E-4	0.12321117937605863	4.970614555185503E-5
44-45	0.004262301981071568	1.6568715183951674E-5	0.0	1.0355446989969797E-4	0.12558050564736373	5.59194137458369E-5
46-47	0.004637169162108475	1.6568715183951674E-5	0.0	1.0355446989969797E-4	0.12769094574391956	7.248812892978857E-5
48-49	0.004802856313947992	1.6568715183951674E-5	0.0	1.1598100628766173E-4	0.12961705888405395	9.941229110371006E-5
50-51	0.005167368047994929	1.6568715183951674E-5	0.0	1.2012318508364963E-4	0.1315845938121482	1.2633645327763153E-4
52-53	0.005511168888061926	1.6568715183951674E-5	0.0	1.2426536387963755E-4	0.13362668795857024	1.470473472575711E-4
54-55	0.005776268331005153	1.6568715183951674E-5	0.0	1.325497214716134E-4	0.13528355947696544	1.6568715183951674E-4
56-57	0.0059999459859885005	1.6568715183951674E-5	0.0	1.325497214716134E-4	0.1367312509661632	2.2574874438134157E-4
58-59	0.0061304246180621195	1.6568715183951674E-5	0.0	1.4083407906358923E-4	0.13799461549893954	2.8166815812717845E-4
60-61	0.006333391379065528	1.6568715183951674E-5	0.0	1.4911843665556506E-4	0.13924969567412387	3.210188566890637E-4
62-63	0.006973358003045662	1.6568715183951674E-5	0.0	1.5326061545155298E-4	0.1406352544813818	3.769382704349006E-4
64-65	0.007692026024149565	1.6568715183951674E-5	0.0	1.574027942475409E-4	0.14171014987894068	4.287155053847496E-4
66-67	0.008348561363313651	1.6568715183951674E-5	0.0	1.6154497304352882E-4	0.14275397893552966	4.784216509366046E-4
68-69	0.008913968768966002	1.6568715183951674E-5	0.0	1.7811368822748052E-4	0.1437998790815166	5.219145282944778E-4
70-71	0.009292978128798896	1.6568715183951674E-5	0.0	1.8225586702346844E-4	0.14480642852894166	5.902604784282784E-4
72-73	0.01033680718538785	1.6568715183951674E-5	0.0	1.8846913521745033E-4	0.145717707864059	6.855305907360006E-4
74-75	0.011020266686725858	2.4853072775927514E-5	0.0	1.946824034114322E-4	0.14667040898713624	7.083125741139341E-4
76-77	0.011440697834518631	2.4853072775927514E-5	0.0	1.988245822074201E-4	0.14758997267984553	7.476632726758194E-4
78-79	0.011625024790940094	2.4853072775927514E-5	0.0	2.0296676100340803E-4	0.14869386332897633	7.932272394316864E-4
80-81	0.011875626608097364	2.4853072775927514E-5	0.0	2.05037850401402E-4	0.1496569198990435	8.408622955855475E-4
82-83	0.01203095831294691	2.4853072775927514E-5	0.0	2.1125111859538387E-4	0.1504750002112511	8.781419047494388E-4
84-85	0.012296057755890138	2.4853072775927514E-5	0.0	2.1125111859538387E-4	0.1514401278707163	9.071371563213542E-4
86-87	0.012393398957595853	2.4853072775927514E-5	0.0	2.1539329739137179E-4	0.15234105175884366	9.609854806691972E-4
88-89	0.012424465298565762	2.4853072775927514E-5	0.0	2.2160656558535368E-4	0.1531177102830914	9.920518216391064E-4
90-91	0.01243274965615774	2.4853072775927514E-5	0.0	2.298909231773295E-4	0.1540124209030248	0.0010541845035789252
92-93	0.012445176192545702	2.4853072775927514E-5	0.0	2.3817528076930535E-4	0.15492370023814214	0.0011059617385287743
94-95	0.012455531639535672	3.313743036790335E-5	0.0	2.402463701672993E-4	0.156000666725099	0.001122530453712726
96-97	0.012472100354719623	3.313743036790335E-5	0.0	2.4231745956529324E-4	0.15695543893757422	0.0011287437219067078
98-99	0.012486597980505582	3.313743036790335E-5	0.0	2.5267290655526305E-4	0.15803033433513308	0.0011598100628766174
100-101	0.012492811248699562	3.313743036790335E-5	0.0	2.609572641472389E-4	0.1592502059905515	0.0012157294766224542
102-103	0.012494882338097556	3.313743036790335E-5	0.0	2.713127111372087E-4	0.1601490587892809	0.0012530090857863453
104-105	0.012509379963883514	3.313743036790335E-5	0.0	2.795970687291845E-4	0.161134897342726	0.00131307067832817
106-107	0.012513522142679503	3.313743036790335E-5	0.0	2.8788142632116037E-4	0.16201925251566945	0.0013648479132780194
108-109	0.012525948679067466	3.313743036790335E-5	0.0	2.9202360511714826E-4	0.16295331383416473	0.001429051684615832
110-111	0.012550801751843393	3.5208519765897307E-5	0.0	3.106634096990939E-4	0.1641317637016233	0.001484971098361669
112-113	0.012575654824619321	3.7279609163891266E-5	0.0	3.293032142810395E-4	0.16507410937771055	0.0015574592272914575
114-115	0.012583939182211297	3.7279609163891266E-5	0.0	3.4587192946499123E-4	0.16599574415981785	0.0015988810152513366
116-117	0.012602578986793244	4.1421787959879184E-5	0.0	3.520851976589731E-4	0.16696087181928304	0.0016320184456192401
118-119	0.012612934433783213	4.763505615386107E-5	0.0	3.58298465852955E-4	0.16787215115440038	0.0017107198427430104
120-121	0.012623289880773183	4.970614555185503E-5	0.0	3.624406446489429E-4	0.16891598021098933	0.0017500705413048957
122-123	0.012631574238365157	4.970614555185503E-5	0.0	3.7072500224091874E-4	0.17004472393289605	0.0018287719384286662
124-125	0.012633645327763152	5.177723494984899E-5	0.0	3.748671810369067E-4	0.17106991318490306	0.0018764069945825272
126-127	0.012635716417161146	5.3848324347842946E-5	0.0	3.790093598328946E-4	0.17212823986727796	0.0019778903750842314
128-129	0.012654356221743091	5.5919413745836905E-5	4.142178795987918E-6	3.872937174248704E-4	0.17332947171811447	0.00205037850401402
130-131	0.012679209294519019	6.420377133781274E-5	4.142178795987918E-6	3.893648068228644E-4	0.17442507800965326	0.0021042268283618625
132-133	0.012679209294519019	6.62748607358067E-5	4.142178795987918E-6	3.893648068228644E-4	0.17542127201008834	0.002151861884515724
134-135	0.012685422562713001	6.62748607358067E-5	4.142178795987918E-6	3.893648068228644E-4	0.1762621343056739	0.0022098523876595546
136-137	0.012687493652110995	6.62748607358067E-5	4.142178795987918E-6	3.893648068228644E-4	0.1773328875244368	0.0022637007120073975
138-139	0.012687493652110995	6.62748607358067E-5	4.142178795987918E-6	3.9557807501684625E-4	0.17829387300510596	0.002288553784783325
>>END_MODULE
