##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24152056
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	67-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.99266273645606	37.0	35.0	38.0	33.0	39.0
2	35.18255555551875	35.0	34.0	38.0	32.0	39.0
3	35.38848787863029	36.0	34.0	38.0	32.0	39.0
4	35.38861788826591	36.0	35.0	38.0	32.0	39.0
5	35.30709029492147	36.0	35.0	38.0	32.0	39.0
6	35.2135185923716	36.0	34.0	37.0	32.0	39.0
7	35.08503412711531	35.0	34.0	37.0	33.0	39.0
8	35.245270464758775	35.0	34.0	37.0	33.0	39.0
9	35.21150973647958	35.0	34.0	37.0	32.0	39.0
10-14	35.249096582088086	35.4	34.2	37.4	32.2	39.0
15-19	35.22718972662203	36.0	34.0	37.0	32.0	39.0
20-24	35.227446060906786	36.0	34.0	37.0	32.0	39.0
25-29	35.2169300534911	36.0	34.0	37.0	32.0	39.0
30-34	35.19024495471524	36.0	34.0	37.0	32.0	39.0
35-39	35.16267842373337	35.8	34.0	37.0	32.0	39.0
40-44	35.14016591382531	35.4	34.0	37.0	32.0	39.0
45-49	35.12683032036692	35.6	34.0	37.0	32.0	39.0
50-54	35.098476518934866	35.2	34.0	37.0	32.0	39.0
55-59	35.075981498221104	35.0	34.0	37.0	32.0	39.0
60-64	35.0550525802027	35.0	34.0	37.0	32.0	39.0
65-69	35.0213120988294	35.0	34.0	37.0	32.0	39.0
70-74	35.00915952709023	35.0	34.0	37.0	32.0	39.0
75-79	34.98001112809399	35.0	34.0	37.0	32.0	39.0
80-84	34.95676045315635	35.0	34.0	37.0	32.0	39.0
85-89	34.928088573612285	35.0	34.0	37.0	32.0	39.0
90-94	34.897708309108744	35.0	34.0	37.0	31.0	39.0
95-99	34.862087027314224	35.0	34.0	37.0	31.0	39.0
100-104	34.83277666596307	35.0	34.0	37.0	31.0	39.0
105-109	34.80678715816491	35.0	34.0	37.0	31.0	39.0
110-114	34.763037814161464	35.0	34.0	37.0	31.0	39.0
115-119	34.73681975723069	35.0	34.0	37.0	31.0	39.0
120-124	34.69778300602283	35.0	34.0	37.0	31.0	39.0
125-129	34.652560258658596	35.0	34.0	37.0	31.0	39.0
130-134	34.6160608622047	35.0	34.0	37.0	30.4	39.0
135-139	34.57381481308757	35.0	34.0	37.0	30.0	39.0
140-144	34.52571009895175	35.0	34.0	37.0	30.0	39.0
145-149	34.53272619855924	35.0	34.0	37.0	30.0	39.0
150	35.203934905042225	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	66.0
23	843.0
24	7016.0
25	34818.0
26	107788.0
27	235116.0
28	385284.0
29	528519.0
30	687341.0
31	901101.0
32	1215161.0
33	1704301.0
34	2574055.0
35	4538768.0
36	1.0169577E7
37	1062282.0
38	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.673095491332084	12.443416825466121	14.947278194452679	36.936209488749114
2	22.35589687827342	12.917596665077143	34.108157764497676	30.61834869215176
3	21.10815298175299	16.983910168558168	27.452958152360686	34.45497869732816
4	24.38917838996988	24.172317568773895	22.339674698831097	29.098829342425127
5	24.512951805657536	30.80256304239472	24.35911311737426	20.32537203457348
6	18.95330068916614	37.535235780842626	25.514537320209584	17.99692620978165
7	15.049553480005661	26.708916231221032	39.76798747725126	18.473542811522048
8	17.35275591509682	26.59568173700051	31.830577047665052	24.220985300237615
9	18.143374363522923	24.901727156685162	32.9540366011092	24.00086187868271
10-14	19.115848902553388	30.797992939906898	27.492164289704395	22.593993867835323
15-19	19.267212087703008	29.192918006188034	28.41051695852598	23.12935294758298
20-24	19.346215364125598	29.317703920855703	28.08757214737981	23.248508567638893
25-29	19.47015001328964	29.384909432509165	27.931589415892006	23.21335113830919
30-34	19.381147283325497	29.320415342026596	27.89822242903121	23.400214945616693
35-39	19.471569348083793	29.13521706497943	28.03836930074357	23.354844286193206
40-44	19.475784355019478	29.192616184390424	27.992827631693483	23.338771828896615
45-49	19.56435535142186	29.031812285905023	27.994929847426224	23.408902515246886
50-54	19.56462149155218	29.095958458968862	27.989183274935698	23.35023677454326
55-59	19.642850680802507	29.034331600327196	27.9462064194466	23.376611299423697
60-64	19.668342056423338	28.941962219927863	27.877452095876816	23.512243627771987
65-69	19.64498516691124	28.864285044658093	28.03834985834433	23.452379930086334
70-74	19.73597615431971	28.8441038990734	28.00555577302059	23.4143641735863
75-79	19.62548750423733	28.84232272454244	28.012265421450564	23.51992434976966
80-84	19.810964426621254	28.719762074902572	27.90214546333885	23.567128035137326
85-89	19.77543131753662	28.75796082796942	28.03184701344732	23.434760841046643
90-94	19.901687846138138	28.64770750856891	27.95702738415937	23.49357726113358
95-99	19.942527002812877	28.636186916440742	27.983001263603345	23.43828481714304
100-104	19.99413338501581	28.55037818566576	27.96455969640143	23.490928732916995
105-109	19.925969261242667	28.50853548981637	28.02789201236806	23.5376032365729
110-114	19.990771619494488	28.55192923361355	27.93811332328368	23.519185823608282
115-119	20.063453920742496	28.48906422439372	28.0049775081484	23.44250434671539
120-124	20.086090460282016	28.339610843817457	28.09982334812543	23.474475347775098
125-129	20.1690263794072	28.351750980547912	28.02627999589502	23.452942644149868
130-134	20.087671577486233	28.46154294002754	27.993664101213785	23.457121381272444
135-139	20.157226987684464	28.32099483007783	28.015408476691288	23.50636970554642
140-144	20.24230569552267	28.24225143656353	28.078113113373686	23.43732975454011
145-149	19.929485016354015	28.207577101412962	28.308642778005737	23.55429510422729
150	20.284231349384385	27.17489248654388	28.41265852090341	24.128217643168323
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	2.0
6	4.0
7	5.0
8	7.0
9	12.5
10	29.5
11	48.0
12	91.5
13	157.5
14	269.5
15	455.5
16	789.0
17	1476.5
18	2981.5
19	5483.5
20	6977.0
21	8904.5
22	13079.0
23	19047.0
24	27794.0
25	39474.0
26	53662.0
27	72708.5
28	100616.0
29	137612.5
30	181959.5
31	233243.0
32	287591.0
33	346787.0
34	417336.0
35	503483.5
36	601671.5
37	712677.5
38	839279.5
39	980501.5
40	1129169.0
41	1274570.5
42	1407934.0
43	1517797.0
44	1598085.5
45	1627770.5
46	1584316.0
47	1488384.0
48	1344080.0
49	1165110.5
50	981448.5
51	801699.5
52	638494.5
53	506323.5
54	405193.0
55	314223.5
56	233962.0
57	166191.0
58	111907.0
59	74286.5
60	52173.0
61	39473.0
62	29199.5
63	19919.0
64	12746.5
65	8523.0
66	5883.0
67	3941.0
68	2451.5
69	1633.5
70	1588.0
71	1968.0
72	2082.5
73	1487.0
74	880.5
75	529.5
76	264.5
77	110.0
78	32.5
79	12.5
80	5.0
81	2.5
82	3.0
83	6.0
84	5.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	1.0
97	1.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.5067775596413E-4
3	6.459077438376261E-4
4	6.624694808591037E-4
5	5.548181902194993E-4
6	4.7614993936748076E-4
7	7.038738234127976E-4
8	6.997333891574283E-4
9	6.831716521359506E-4
10-14	7.999318981373676E-4
15-19	6.028472275817844E-4
20-24	5.77176535198494E-4
25-29	6.450796569865522E-4
30-34	3.57733519663916E-4
35-39	3.345470878338473E-4
40-44	1.3911859098041178E-4
45-49	1.7058589132121918E-4
50-54	2.1033406017276543E-4
55-59	3.055640480462616E-4
60-64	2.6498779234364144E-4
65-69	1.6230502684256017E-4
70-74	9.357382501956397E-5
75-79	1.854915775230581E-4
80-84	4.885720472479643E-5
85-89	1.9956968800434303E-4
90-94	7.701268745933595E-5
95-99	5.2170160248348516E-5
100-104	7.038873054230864E-5
105-109	1.1924773559729577E-4
110-114	5.2999518954834914E-5
115-119	1.4657829734401366E-4
120-124	1.3333011684778156E-4
125-129	9.358102956507124E-5
130-134	1.1842818403945185E-4
135-139	2.1781820317987578E-4
140-144	0.027704331894499636
145-149	8.826028141916816E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
66-67	1.0
68-69	1.0
70-71	0.0
72-73	4.0
74-75	6.0
76-77	8.0
78-79	8.0
80-81	10.0
82-83	15.0
84-85	23.0
86-87	34.0
88-89	33.0
90-91	47.0
92-93	53.0
94-95	50.0
96-97	54.0
98-99	50.0
100-101	61.0
102-103	82.0
104-105	74.0
106-107	85.0
108-109	97.0
110-111	95.0
112-113	108.0
114-115	90.0
116-117	97.0
118-119	140.0
120-121	148.0
122-123	144.0
124-125	158.0
126-127	166.0
128-129	192.0
130-131	244.0
132-133	320.0
134-135	419.0
136-137	615.0
138-139	1515.0
140-141	3593.0
142-143	5641.0
144-145	49206.0
146-147	620080.0
148-149	998776.0
150-151	2.2469513E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.0629876897527
#Duplication Level	Percentage of total
1	38.27282347764292
2	11.360554823331535
3	6.609506073154218
4	4.446150578964936
5	3.366991819035852
6	2.6008040422344036
7	2.103208946896653
8	1.7042965722504972
9	1.5301293672058216
>10	16.580453802624827
>50	4.548835599839436
>100	5.562451101379271
>500	0.8477306269380899
>1k	0.46606316850148477
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	4.140434255369398E-6	1.3249389617182075E-4	1.2007259340571255E-4
2	0.0	0.0	0.0	8.280868510738797E-6	0.001486415897677614	3.353751746849212E-4
3	4.140434255369398E-6	0.0	0.0	8.280868510738797E-6	0.002620894883648829	4.3474559681378676E-4
4	2.4842605532216387E-5	0.0	0.0	8.280868510738797E-6	0.0040493447017512715	5.051329791550665E-4
5	2.8983039787585787E-5	0.0	0.0	1.2421302766108193E-5	0.005647552324323859	5.548181902194993E-4
6	3.3123474042955187E-5	0.0	0.0	1.2421302766108193E-5	0.0067820313102950735	6.127842697946709E-4
7	3.3123474042955187E-5	0.0	0.0	1.2421302766108193E-5	0.007862684650946486	6.707503493698424E-4
8	3.3123474042955187E-5	0.0	0.0	1.6561737021477593E-5	0.009787986579693257	7.49418600221861E-4
9	4.968521106443277E-5	0.0	0.0	1.6561737021477593E-5	0.012996823127604541	8.363677195846184E-4
10-11	5.796607957517157E-5	0.0	0.0	2.070217127684699E-5	0.01761754775659679	9.667913986287544E-4
12-13	6.003629670285627E-5	2.070217127684699E-6	0.0	2.2772388404531687E-5	0.021099652965362453	0.0010661618207576198
14-15	6.210651383054097E-5	4.140434255369398E-6	0.0	3.1053256915270487E-5	0.025111733758815397	0.0011551811572480621
16-17	6.417673095822567E-5	4.140434255369398E-6	0.0	3.726390829832458E-5	0.02861454113885791	0.001250411145121558
18-19	6.831716521359506E-5	4.140434255369398E-6	0.0	3.726390829832458E-5	0.03169088379059737	0.0013394304816120002
20-21	8.073846797970326E-5	4.140434255369398E-6	0.0	4.554477680906338E-5	0.034895579904253286	0.0014056774296979106
22-23	8.487890223507266E-5	4.140434255369398E-6	0.0	4.968521106443277E-5	0.03774212845481975	0.0014946967661883526
24-25	8.694911936275736E-5	4.140434255369398E-6	0.0	4.968521106443277E-5	0.040503798103151134	0.0016375417479985968
26-27	8.694911936275736E-5	4.140434255369398E-6	0.0	5.382564531980217E-5	0.04405836091138576	0.0017369121701274624
28-29	9.108955361812676E-5	4.140434255369398E-6	0.0	6.210651383054097E-5	0.04748664047483163	0.0018093697695964269
30-31	9.108955361812676E-5	4.140434255369398E-6	0.0	6.210651383054097E-5	0.05112815240242902	0.0018942486718314996
32-33	9.522998787349615E-5	4.140434255369398E-6	0.0	8.694911936275736E-5	0.05445292110949064	0.002014321265237212
34-35	9.522998787349615E-5	4.140434255369398E-6	0.0	9.108955361812676E-5	0.057742496125381626	0.002150955595664402
36-37	9.730020500118085E-5	4.140434255369398E-6	0.0	9.108955361812676E-5	0.060502095556585325	0.0022234131951333665
38-39	1.0765129063960435E-4	4.140434255369398E-6	0.0	9.522998787349615E-5	0.063334152587258	0.002297941011730016
40-41	1.0765129063960435E-4	4.140434255369398E-6	0.0	9.522998787349615E-5	0.06653056783240317	0.0024035220852419354
42-43	1.1179172489497375E-4	4.140434255369398E-6	0.0	9.522998787349615E-5	0.06944336333105554	0.0025111733758815397
44-45	1.1593215915034315E-4	4.140434255369398E-6	0.0	9.522998787349615E-5	0.07234994817832485	0.002585701192478189
46-47	1.2007259340571255E-4	4.140434255369398E-6	0.0	9.522998787349615E-5	0.07510126674101783	0.0026498779234364147
48-49	1.2007259340571255E-4	4.140434255369398E-6	0.0	9.522998787349615E-5	0.07870137432606152	0.0027244057400330635
50-51	1.2007259340571255E-4	4.140434255369398E-6	0.0	9.522998787349615E-5	0.08156241439652176	0.0027803016024805505
52-53	1.2421302766108195E-4	4.140434255369398E-6	0.0	9.937042212886555E-5	0.08431994361059779	0.0028465485505664613
54-55	1.3249389617182075E-4	4.140434255369398E-6	0.0	1.0144063925655025E-4	0.08699466413956641	0.002904514630141633
56-57	1.3663433042719012E-4	4.140434255369398E-6	0.0	1.1800237627802785E-4	0.0899198809409849	0.0029541998412060655
58-59	1.428449818102442E-4	4.140434255369398E-6	0.0	1.2421302766108195E-4	0.09235031584888673	0.002989393532376705
60-61	1.4698541606561363E-4	4.140434255369398E-6	0.0	1.2628324478876666E-4	0.0950084746408339	0.0030556404804626155
62-63	1.594067188317218E-4	4.140434255369398E-6	0.0	1.2835346191645135E-4	0.09758796518192903	0.0031757130738683283
64-65	1.6561737021477592E-4	4.140434255369398E-6	0.0	1.2835346191645135E-4	0.1002440537567485	0.003248170673337293
66-67	1.7389823872551472E-4	6.2106513830540975E-6	0.0	1.2835346191645135E-4	0.10304505753050588	0.003301996318657095
68-69	1.7803867298088412E-4	8.280868510738797E-6	0.0	1.2835346191645135E-4	0.10560384590032418	0.003359962398232267
70-71	1.842493243639382E-4	8.280868510738797E-6	0.0	1.2835346191645135E-4	0.10799908711705539	0.003424139129190492
72-73	1.946004100023617E-4	8.280868510738797E-6	0.0	1.2835346191645135E-4	0.11037983681389278	0.0034986669457871414
74-75	1.987408442577311E-4	1.2421302766108193E-5	0.0	1.2835346191645135E-4	0.11277921846487934	0.003616669322065169
76-77	2.008110613854158E-4	1.6561737021477593E-5	0.0	1.2835346191645135E-4	0.11543944747395418	0.003684986487278764
78-79	2.028812785131005E-4	1.6561737021477593E-5	0.0	1.2835346191645135E-4	0.1181845553852641	0.0037201801784494037
80-81	2.111621470238393E-4	1.6561737021477593E-5	0.0	1.2835346191645135E-4	0.12091517177668021	0.0037470930011093053
82-83	2.1323236415152398E-4	1.6561737021477593E-5	4.140434255369398E-6	1.2835346191645135E-4	0.12366649033937317	0.003800918646429107
84-85	2.194430155345781E-4	1.6561737021477593E-5	4.140434255369398E-6	1.2835346191645135E-4	0.12625840218323442	0.0038816571144088107
86-87	2.256536669176322E-4	1.6561737021477593E-5	4.140434255369398E-6	1.2835346191645135E-4	0.12924158506422806	0.003929272108345559
88-89	2.2979410117300158E-4	1.6561737021477593E-5	4.140434255369398E-6	1.2835346191645135E-4	0.13188525233628146	0.003981027536537676
90-91	2.318643183006863E-4	1.6561737021477593E-5	4.140434255369398E-6	1.2835346191645135E-4	0.13449165570003646	0.004028642530474424
92-93	2.33934535428371E-4	1.6561737021477593E-5	4.140434255369398E-6	1.2835346191645135E-4	0.13671299867804215	0.004109380998454128
94-95	2.360047525560557E-4	1.6561737021477593E-5	4.140434255369398E-6	1.2835346191645135E-4	0.1387107582062579	0.004192189683561515
96-97	2.401451868114251E-4	1.6561737021477593E-5	4.140434255369398E-6	1.2835346191645135E-4	0.14075820294553804	0.004248085546009002
98-99	2.504962724498486E-4	1.6561737021477593E-5	4.140434255369398E-6	1.2835346191645135E-4	0.14242265751619654	0.0042977707570734346
100-101	2.5256648957753327E-4	2.070217127684699E-5	4.140434255369398E-6	1.2835346191645135E-4	0.1443914340046247	0.004345385751010183
102-103	2.54636706705218E-4	2.070217127684699E-5	4.140434255369398E-6	1.2835346191645135E-4	0.14612834617475218	0.004397141179202301
104-105	2.5877714096058735E-4	2.070217127684699E-5	4.140434255369398E-6	1.3042367904413603E-4	0.1478714689962627	0.004442685956011364
106-107	2.6084735808827207E-4	2.070217127684699E-5	4.140434255369398E-6	1.428449818102442E-4	0.14955869595532573	0.004486160515692743
108-109	2.6084735808827207E-4	2.070217127684699E-5	4.140434255369398E-6	1.5319606744866772E-4	0.15170965155099012	0.004523424423991067
110-111	2.649877923436415E-4	2.070217127684699E-5	6.2106513830540975E-6	1.7182802159783003E-4	0.15404278625389076	0.0045813905035662385
112-113	2.649877923436415E-4	2.070217127684699E-5	8.280868510738797E-6	1.8010889010856883E-4	0.15587078797763637	0.004635216148886041
114-115	2.649877923436415E-4	2.070217127684699E-5	8.280868510738797E-6	1.8217910723625352E-4	0.15739653800074	0.00467455027431205
116-117	2.7326866085438024E-4	2.4842605532216387E-5	8.280868510738797E-6	1.8217910723625352E-4	0.1589492008465035	0.0047221652682487986
118-119	2.7326866085438024E-4	2.4842605532216387E-5	8.280868510738797E-6	1.904599757469923E-4	0.1605991638972682	0.004755288742291754
120-121	2.7533887798206495E-4	2.4842605532216387E-5	8.280868510738797E-6	1.946004100023617E-4	0.16229881215909736	0.004804973953356186
122-123	2.7740909510974967E-4	2.4842605532216387E-5	8.280868510738797E-6	1.9667062713004638E-4	0.16445804862327248	0.004867080467186728
124-125	2.794793122374344E-4	2.4842605532216387E-5	8.280868510738797E-6	2.008110613854158E-4	0.16604590516020667	0.004906414592612736
126-127	2.8154952936511904E-4	2.4842605532216387E-5	8.280868510738797E-6	2.1323236415152398E-4	0.16758614670320404	0.004933327415272638
128-129	2.8568996362048847E-4	2.4842605532216387E-5	8.280868510738797E-6	2.153025812792087E-4	0.16924853105673487	0.004956099803677169
130-131	2.8568996362048847E-4	2.4842605532216387E-5	8.280868510738797E-6	2.153025812792087E-4	0.1708922834561165	0.00498715306059244
132-133	2.8568996362048847E-4	2.8983039787585787E-5	8.280868510738797E-6	2.153025812792087E-4	0.17226690762889918	0.0050161361003800255
134-135	2.8983039787585784E-4	2.8983039787585787E-5	8.280868510738797E-6	2.194430155345781E-4	0.17379472786913047	0.005040978705912242
136-137	2.8983039787585784E-4	2.8983039787585787E-5	8.280868510738797E-6	2.194430155345781E-4	0.17571795958074954	0.005057540442933719
138-139	2.8983039787585784E-4	2.8983039787585787E-5	8.280868510738797E-6	2.194430155345781E-4	0.17717332222151191	0.005074102179955197
>>END_MODULE
