##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24152056
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	59-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.32775073062103	37.0	35.0	38.0	34.0	39.0
2	35.58033916450012	36.0	35.0	38.0	33.0	39.0
3	35.658935578817804	36.0	35.0	37.0	33.0	39.0
4	35.793825999740974	36.0	35.0	38.0	34.0	39.0
5	35.68692963447915	36.0	35.0	38.0	33.0	39.0
6	35.64653046514963	36.0	35.0	37.0	33.0	39.0
7	35.5515625667645	35.0	34.0	37.0	33.0	39.0
8	35.73302753190039	36.0	35.0	38.0	33.0	39.0
9	35.714897025743895	36.0	35.0	38.0	33.0	39.0
10-14	35.68985600232129	36.0	35.0	37.8	33.0	39.0
15-19	35.64172144185157	36.0	35.0	38.0	33.0	39.0
20-24	35.611470418915886	36.0	35.0	38.0	33.0	39.0
25-29	35.56912840877812	36.0	35.0	38.0	33.0	39.0
30-34	35.53564306906212	36.0	35.0	38.0	33.0	39.0
35-39	35.50357234183293	36.0	35.0	38.0	33.0	39.0
40-44	35.46338394544961	36.0	35.0	38.0	33.0	39.0
45-49	35.41915899002553	36.0	34.4	38.0	33.0	39.0
50-54	35.37449803859348	36.0	34.0	38.0	32.0	39.0
55-59	35.33449789119402	36.0	34.0	38.0	32.0	39.0
60-64	35.282487583418	36.0	34.0	38.0	32.0	39.0
65-69	35.230984185659736	36.0	34.0	38.0	32.0	39.0
70-74	35.171610796274976	36.0	34.0	38.0	32.0	39.0
75-79	35.11095386173485	36.0	34.0	38.0	32.0	39.0
80-84	35.04829005673663	36.0	34.0	38.0	31.6	39.0
85-89	34.965299558208514	35.8	34.0	37.6	31.0	39.0
90-94	34.89340465930739	35.0	34.0	37.0	31.0	39.0
95-99	34.82923614721581	35.0	34.0	37.0	31.0	39.0
100-104	34.77006150939466	35.0	34.0	37.0	30.8	39.0
105-109	34.65862698940229	35.0	34.0	37.0	30.0	39.0
110-114	34.58198219685475	35.0	34.0	37.0	30.0	39.0
115-119	34.49227396752359	35.0	34.0	37.0	30.0	39.0
120-124	34.42685590761572	35.0	34.0	37.0	29.8	39.0
125-129	34.29280926282881	35.0	34.0	37.0	29.0	39.0
130-134	34.19759262505071	35.0	34.0	37.0	29.0	39.0
135-139	34.08663634264465	35.0	34.0	37.0	28.2	39.0
140-144	33.95065474290007	35.0	33.0	37.0	28.0	39.0
145-149	33.916769473827244	35.0	33.0	37.0	28.0	39.0
150	34.8356946143156	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	36.0
21	353.0
22	2820.0
23	13685.0
24	44145.0
25	100414.0
26	181433.0
27	288291.0
28	402071.0
29	508832.0
30	635073.0
31	806137.0
32	1057747.0
33	1471022.0
34	2243824.0
35	4034684.0
36	1.0894996E7
37	1466490.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.35836270005336	20.093746884323224	16.836475536492628	28.711414879130785
2	23.63737992481385	25.462967850975826	33.416270375336595	17.48338184887373
3	20.475871149942517	28.7797611481464	31.63970566132669	19.104662040584394
4	23.87600703315675	32.90130220058177	24.0372325374507	19.185458228810784
5	25.0346145623554	34.952219902493454	23.708729345110598	16.30443619004055
6	19.921579347116452	38.707686004040404	24.14685110037837	17.223883548464777
7	20.471064658015038	21.66687589661104	38.98657323417932	18.87548621119461
8	22.101729144715463	23.773984293511077	30.378519327712723	23.745767234060736
9	23.28959488997541	23.17631674918276	30.254095137904613	23.27999322293721
10-14	22.808874739276856	29.537482026374896	26.9405544604567	20.713088773891545
15-19	22.740410174603767	28.38088566869835	28.495708191468257	20.38299596522963
20-24	22.70895623581513	28.561445392534925	28.230467345325273	20.499131026324672
25-29	22.80580188078196	28.557998675402974	28.1496958679499	20.486503575865157
30-34	22.78278420686007	28.42469974398867	28.326164861492536	20.466351187658724
35-39	22.86819888128779	28.440853234192566	28.16568991062293	20.525257973896714
40-44	23.07909272817188	28.217908239364796	28.179539663207144	20.523459369256184
45-49	23.07673185255947	28.15257053064137	28.21639035616678	20.554307260632388
50-54	23.135171597813454	28.153730680319722	28.18347141957604	20.527626302290784
55-59	23.250932342985625	27.982214847464746	28.208314025108255	20.558538784441375
60-64	23.124176581447635	27.941095098873326	28.294022419988877	20.64070589969016
65-69	23.152833380955247	27.957978882700917	28.330246293409417	20.558941442934422
70-74	23.360529399981502	27.876431215572754	28.22287757107873	20.540161813367014
75-79	23.234339624107072	27.880158689615158	28.336663402306666	20.5488382839711
80-84	23.353843536046636	27.972398698015983	28.114205004830854	20.559552761106524
85-89	23.372721667072078	27.91685749610696	28.230108166319724	20.480312670501235
90-94	23.33222520529422	27.92168885868073	28.244099628108543	20.50198630791651
95-99	23.28284377893545	28.01342882358295	28.275094533343037	20.428632864138564
100-104	23.457586820800834	27.962908798422077	28.150543521982165	20.428960858794927
105-109	23.25873584157665	28.014813744181605	28.368264784765863	20.358185629475884
110-114	23.33596623614182	27.992736424955734	28.282298310221037	20.38899902868141
115-119	23.40057804928452	27.97883357291029	28.37192448004347	20.248663897761727
120-124	23.43542294154039	27.989014516847256	28.392770566845122	20.18279197476723
125-129	23.383670394409336	28.164505069201567	28.26465337972548	20.187171156663613
130-134	23.511692376055464	28.153157917058518	28.215336409888277	20.119813296997744
135-139	23.323270403247143	28.143718182159077	28.435961781610565	20.097049632983218
140-144	23.27931287379175	28.2243329458421	28.337532914835723	20.158821265530428
145-149	23.144919193778666	28.089392477462738	28.43843525406496	20.327253074693637
150	23.695088140702637	26.959589970131454	28.576855940728624	20.76846594843729
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	2.5
7	7.0
8	11.0
9	15.0
10	26.5
11	37.0
12	61.0
13	107.5
14	176.0
15	290.0
16	452.5
17	792.0
18	1339.5
19	2177.5
20	3410.5
21	5320.0
22	7907.0
23	11479.0
24	16797.0
25	24984.5
26	41645.5
27	57927.5
28	72604.0
29	98821.0
30	131580.0
31	166682.5
32	214292.5
33	277901.5
34	354315.0
35	448559.0
36	569826.5
37	711498.0
38	858421.0
39	1023661.0
40	1205873.5
41	1376620.0
42	1524221.0
43	1624730.5
44	1664506.5
45	1673252.5
46	1626256.0
47	1499174.0
48	1330931.5
49	1136512.0
50	945622.5
51	774743.5
52	617190.0
53	503955.0
54	414037.0
55	323031.0
56	239956.0
57	169812.0
58	118144.0
59	78875.5
60	55464.0
61	42622.5
62	30991.0
63	21025.5
64	14525.5
65	10094.0
66	6842.0
67	4721.0
68	3465.0
69	2580.5
70	1946.5
71	1550.0
72	1247.0
73	1038.5
74	1135.5
75	1108.5
76	725.5
77	334.5
78	157.0
79	76.0
80	16.0
81	5.0
82	1.5
83	1.5
84	1.5
85	0.5
86	0.5
87	0.5
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.1404342553693975E-6
3	5.796607957517157E-5
4	7.038738234127976E-4
5	0.12414264027874067
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	4.1404342553693975E-6
25-29	0.04920492069080992
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.281570824171742E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
58-59	1.0
60-61	0.0
62-63	1.0
64-65	3.0
66-67	6.0
68-69	11.0
70-71	9.0
72-73	22.0
74-75	35.0
76-77	33.0
78-79	52.0
80-81	60.0
82-83	84.0
84-85	95.0
86-87	113.0
88-89	124.0
90-91	160.0
92-93	180.0
94-95	215.0
96-97	225.0
98-99	269.0
100-101	349.0
102-103	360.0
104-105	360.0
106-107	400.0
108-109	436.0
110-111	459.0
112-113	466.0
114-115	522.0
116-117	550.0
118-119	588.0
120-121	656.0
122-123	804.0
124-125	886.0
126-127	1030.0
128-129	1176.0
130-131	1394.0
132-133	1696.0
134-135	2154.0
136-137	2893.0
138-139	5076.0
140-141	8239.0
142-143	12320.0
144-145	62448.0
146-147	736636.0
148-149	1106175.0
150-151	2.2202285E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.596337688705304
#Duplication Level	Percentage of total
1	28.130995665988124
2	11.300300233184986
3	6.964777779347409
4	5.035061608463573
5	3.7885866670748274
6	2.9233390116424283
7	2.3936887974175214
8	1.94599412073537
9	1.7584713000615881
>10	19.802523415454523
>50	5.58280993382917
>100	7.78867019365377
>500	1.4235228675471467
>1k	1.1108771869164618
>5k	0.05038121868304102
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2421302766108193E-5	0.0	0.0	0.0	0.0015650841485296324	4.140434255369398E-6
2	1.0765129063960435E-4	0.0	0.0	8.280868510738797E-6	0.011406896373542691	8.280868510738797E-6
3	3.3123474042955184E-4	0.0	0.0	8.280868510738797E-6	0.013609607397399212	1.2421302766108193E-5
4	4.968521106443278E-4	0.0	0.0	8.280868510738797E-6	0.01657829875849907	1.2421302766108193E-5
5	5.755203614963464E-4	0.0	0.0	8.280868510738797E-6	0.02009766787556306	1.2421302766108193E-5
6	6.417673095822567E-4	0.0	0.0	1.2421302766108193E-5	0.022329361939207162	1.6561737021477593E-5
7	7.121546919235365E-4	0.0	0.0	1.2421302766108193E-5	0.02502478463945264	1.6561737021477593E-5
8	7.866825085201856E-4	0.0	0.0	1.2421302766108193E-5	0.03037836613164527	1.6561737021477593E-5
9	0.0012048663683124948	0.0	0.0	1.2421302766108193E-5	0.040948894785603346	1.6561737021477593E-5
10-11	0.0016727354391692368	0.0	0.0	1.2421302766108193E-5	0.0487618942254854	1.6561737021477593E-5
12-13	0.0017369121701274624	0.0	0.0	2.2772388404531687E-5	0.05474068129023881	1.6561737021477593E-5
14-15	0.0018217910723625351	0.0	0.0	2.4842605532216387E-5	0.07136452482554695	1.6561737021477593E-5
16-17	0.0018735465005546527	0.0	0.0	2.4842605532216387E-5	0.0860113110039162	1.8631954149162293E-5
18-19	0.002173727984068934	0.0	0.0	3.1053256915270487E-5	0.09181205939568872	2.8983039787585787E-5
20-21	0.0026312459692872524	0.0	0.0	3.726390829832458E-5	0.09819246858321296	3.3123474042955187E-5
22-23	0.003094974605888625	0.0	0.0	4.554477680906337E-5	0.10450249038839592	3.726390829832458E-5
24-25	0.003144659816953058	4.140434255369398E-6	0.0	5.382564531980217E-5	0.10933851759866738	3.726390829832458E-5
26-27	0.0031902045937621214	4.140434255369398E-6	0.0	5.796607957517157E-5	0.11370874595520977	4.347455968137868E-5
28-29	0.0033951560894029064	4.140434255369398E-6	0.0	5.796607957517157E-5	0.1188863589915492	5.175542819211747E-5
30-31	0.003697407790044872	4.140434255369398E-6	0.0	5.796607957517157E-5	0.12219249574446167	5.796607957517157E-5
32-33	0.003728461046960143	4.140434255369398E-6	0.0	6.003629670285627E-5	0.12588369288312348	6.003629670285627E-5
34-35	0.003877516680153441	4.140434255369398E-6	0.0	7.038738234127976E-5	0.1293720087432722	6.831716521359506E-5
36-37	0.003949974279622405	4.140434255369398E-6	0.0	8.694911936275736E-5	0.13170307322904518	7.452781659664916E-5
38-39	0.0040700468730281185	4.140434255369398E-6	0.0	8.694911936275736E-5	0.13373395623130385	7.452781659664916E-5
40-41	0.004134223603986345	4.140434255369398E-6	0.0	1.0351085638423495E-4	0.1361126357110136	8.073846797970326E-5
42-43	0.004438545521755995	4.140434255369398E-6	0.0	1.1593215915034315E-4	0.13836089151167918	8.901933649044206E-5
44-45	0.004862940032931359	4.140434255369398E-6	0.0	1.1593215915034315E-4	0.14101698008649866	9.522998787349615E-5
46-47	0.005281123892723667	4.140434255369398E-6	0.0	1.3456411329950543E-4	0.14330664022971792	1.0558107351191965E-4
48-49	0.005465373217087606	4.140434255369398E-6	0.0	1.4491519893792892E-4	0.14523608259272006	1.1800237627802785E-4
50-51	0.005844222951453905	4.140434255369398E-6	0.0	1.4491519893792892E-4	0.14730629972040477	1.3456411329950543E-4
52-53	0.0061795981261388265	4.140434255369398E-6	0.0	1.4491519893792892E-4	0.14970154093713595	1.5319606744866772E-4
54-55	0.006461147655503945	4.140434255369398E-6	0.0	1.4905563319329832E-4	0.1513432231193899	1.8010889010856883E-4
56-57	0.006676450236783154	4.140434255369398E-6	0.0	1.5319606744866772E-4	0.15294764139334555	1.92530192874677E-4
58-59	0.006815154784338029	4.140434255369398E-6	0.0	1.5733650170403712E-4	0.15424566753240387	2.153025812792087E-4
60-61	0.007082212793809355	4.140434255369398E-6	0.0	1.6561737021477592E-4	0.15552092128305767	2.4221540393910978E-4
62-63	0.007786086617222153	4.140434255369398E-6	0.0	1.6561737021477592E-4	0.15698663500945842	2.877601807481731E-4
64-65	0.008665928896488151	4.140434255369398E-6	0.0	1.7182802159783003E-4	0.15827845049713365	3.3951560894029064E-4
66-67	0.009299415337559668	1.2421302766108193E-5	0.0	1.8217910723625352E-4	0.15939843796321107	3.829901686216693E-4
68-69	0.009839742007885375	1.2421302766108193E-5	0.0	1.946004100023617E-4	0.1603693697960952	4.2646472830304796E-4
70-71	0.01014613414278271	1.2421302766108193E-5	0.0	1.9667062713004638E-4	0.16151005943344948	4.865010250059042E-4
72-73	0.011210225746412644	1.2421302766108193E-5	0.0	1.987408442577311E-4	0.16243751670665224	5.486075388364453E-4
74-75	0.011876835661527118	1.2421302766108193E-5	0.0	1.987408442577311E-4	0.16334841224283347	5.734501443686616E-4
76-77	0.012386109074937554	1.2421302766108193E-5	0.0	2.008110613854158E-4	0.16446632949178322	6.169247040500403E-4
78-79	0.012586920136322968	1.2421302766108193E-5	0.0	2.028812785131005E-4	0.16566291499158498	6.562588294760495E-4
80-81	0.012827065323134395	1.2421302766108193E-5	0.0	2.0495149564078518E-4	0.1666711107327674	6.686801322421578E-4
82-83	0.013069280727073505	1.2421302766108193E-5	0.0	2.070217127684699E-4	0.16767309582256681	7.121546919235365E-4
84-85	0.013402585684630743	1.2421302766108193E-5	0.0	2.070217127684699E-4	0.16860469353002494	7.390675145834375E-4
86-87	0.0135164476266534	1.2421302766108193E-5	0.0	2.070217127684699E-4	0.1695321508032277	7.576994687325997E-4
88-89	0.013543360449313301	1.2421302766108193E-5	0.0	2.090919298961546E-4	0.1705299954587717	8.032442455416632E-4
90-91	0.013557851969207092	1.2421302766108193E-5	0.0	2.194430155345781E-4	0.17160857858229545	8.446485880953573E-4
92-93	0.013572343489100887	1.2421302766108193E-5	0.0	2.2979410117300158E-4	0.1727886023450757	8.901933649044205E-4
94-95	0.013576483923356256	1.2421302766108193E-5	0.0	2.318643183006863E-4	0.17388995785700398	9.233168389473757E-4
96-97	0.013576483923356256	1.2421302766108193E-5	0.0	2.33934535428371E-4	0.175038928362869	9.585105301180155E-4
98-99	0.013586835008994678	1.6561737021477593E-5	0.0	2.4428562106679447E-4	0.17615270517756335	9.937042212886556E-4
100-101	0.013601326528888472	1.6561737021477593E-5	0.0	2.4428562106679447E-4	0.17741967805970638	0.0010268276953316106
102-103	0.013601326528888472	1.6561737021477593E-5	0.0	2.484260553221639E-4	0.17843408445227188	0.0010765129063960435
104-105	0.01362202870016532	1.6561737021477593E-5	0.0	2.5877714096058735E-4	0.1794319291078159	0.001148970505865008
106-107	0.013634450002931427	1.6561737021477593E-5	0.0	2.649877923436415E-4	0.18039458007218928	0.001182093979907963
108-109	0.013659292608463644	1.6561737021477593E-5	0.0	2.7740909510974967E-4	0.18136137147081804	0.0012297089738447114
110-111	0.013677924562612806	1.6561737021477593E-5	0.0	2.8983039787585784E-4	0.18248342915402316	0.0012897452705475675
112-113	0.013717258688038816	1.6561737021477593E-5	0.0	2.9397083213122727E-4	0.1834522907697796	0.0013435709158673697
114-115	0.013748311944954086	1.6561737021477593E-5	0.0	2.96041049258912E-4	0.18434869478606708	0.001403607212570226
116-117	0.013756592813464825	2.070217127684699E-5	4.140434255369398E-6	3.02251700641966E-4	0.1853879437841648	0.001436730686613181
118-119	0.01378143541899704	2.070217127684699E-5	4.140434255369398E-6	3.1053256915270487E-4	0.18629469888609068	0.0015091882860821457
120-121	0.01379385672176315	2.070217127684699E-5	4.140434255369398E-6	3.1053256915270487E-4	0.18726977115323018	0.0015692245827850018
122-123	0.013800067373146204	2.070217127684699E-5	4.140434255369398E-6	3.1053256915270487E-4	0.1884746375215427	0.0016623843535308134
124-125	0.013808348241656943	2.070217127684699E-5	4.140434255369398E-6	3.1053256915270487E-4	0.1895283780395342	0.0016955078275737684
126-127	0.013818699327295365	2.070217127684699E-5	4.140434255369398E-6	3.126027862803896E-4	0.1905138013923121	0.0017596845585319942
128-129	0.01382490997867842	2.070217127684699E-5	4.140434255369398E-6	3.1467300340807424E-4	0.1916772634180709	0.0018072995524687423
130-131	0.013829050412933789	2.070217127684699E-5	4.140434255369398E-6	3.1467300340807424E-4	0.19281795305542515	0.0018549145464054903
132-133	0.013831120630061474	2.4842605532216387E-5	4.140434255369398E-6	3.1881343766344367E-4	0.19385306161926752	0.0018901082375761301
134-135	0.013837331281444529	2.4842605532216387E-5	4.140434255369398E-6	3.1881343766344367E-4	0.19478465932672565	0.0019232317116190853
136-137	0.013841471715699898	2.4842605532216387E-5	4.140434255369398E-6	3.2295387191881304E-4	0.19581148702205725	0.0019667062713004642
138-139	0.013841471715699898	2.4842605532216387E-5	4.140434255369398E-6	3.2502408904649775E-4	0.19676792733504758	0.0020101808309818427
>>END_MODULE
