##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.2.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24104263
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	61-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.02607352068802	37.0	35.0	38.0	33.0	39.0
2	35.230670027123416	35.0	34.0	38.0	32.0	39.0
3	35.429114883122544	36.0	34.0	38.0	32.0	39.0
4	35.43084395486392	36.0	35.0	38.0	32.0	39.0
5	35.34358980401102	36.0	35.0	38.0	32.0	39.0
6	35.25171821266637	36.0	34.0	37.0	33.0	39.0
7	35.113384715392456	35.0	34.0	37.0	33.0	39.0
8	35.28886462946409	35.0	34.0	37.0	33.0	39.0
9	35.25433451335973	35.0	34.0	37.0	33.0	39.0
10-14	35.29242371774653	35.6	34.2	37.4	32.8	39.0
15-19	35.266735539684404	36.0	34.0	37.0	32.2	39.0
20-24	35.271495403116035	36.0	34.0	37.0	32.0	39.0
25-29	35.25755951965841	36.0	34.0	37.0	32.0	39.0
30-34	35.23424497981954	36.0	34.0	37.0	32.0	39.0
35-39	35.20198324254925	36.0	34.0	37.0	32.0	39.0
40-44	35.18491431992756	36.0	34.0	37.0	32.0	39.0
45-49	35.167438490029745	36.0	34.0	37.0	32.0	39.0
50-54	35.14298595231889	36.0	34.0	37.0	32.0	39.0
55-59	35.11711826244179	35.8	34.0	37.0	32.0	39.0
60-64	35.09816457177045	35.6	34.0	37.0	32.0	39.0
65-69	35.06203855567119	35.0	34.0	37.0	32.0	39.0
70-74	35.05040947246224	35.0	34.0	37.0	32.0	39.0
75-79	35.01872022405802	35.0	34.0	37.0	32.0	39.0
80-84	34.99808730590703	35.0	34.0	37.0	32.0	39.0
85-89	34.96797803254806	35.0	34.0	37.0	32.0	39.0
90-94	34.938675162546296	35.0	34.0	37.0	32.0	39.0
95-99	34.90171121515222	35.0	34.0	37.0	31.2	39.0
100-104	34.87353663464549	35.0	34.0	37.0	31.0	39.0
105-109	34.848131378257946	35.0	34.0	37.0	31.0	39.0
110-114	34.8044033185415	35.0	34.0	37.0	31.0	39.0
115-119	34.7786102011717	35.0	34.0	37.0	31.0	39.0
120-124	34.740127130179374	35.0	34.0	37.0	31.0	39.0
125-129	34.69844312752055	35.0	34.0	37.0	31.0	39.0
130-134	34.66303284773634	35.0	34.0	37.0	31.0	39.0
135-139	34.62239327231555	35.0	34.0	37.0	30.4	39.0
140-144	34.575715170470765	35.0	34.0	37.0	30.0	39.0
145-149	34.58468280910694	35.0	34.0	37.0	30.2	39.0
150	35.24322055511305	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	40.0
23	850.0
24	6686.0
25	33010.0
26	102067.0
27	222855.0
28	369712.0
29	510199.0
30	664282.0
31	872755.0
32	1181306.0
33	1669472.0
34	2544307.0
35	4517021.0
36	1.0305581E7
37	1104100.0
38	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.6308923446446	12.367596553356558	15.46425626039676	36.537254841602085
2	22.56819674678838	12.876031685704836	33.97972769716247	30.576043870344314
3	21.31721730358345	17.07695826401772	27.47407424217199	34.13175019022684
4	24.513574171615552	24.323432135116015	22.297244541859	28.86574915140943
5	24.466761900806837	31.07957853313011	24.262871284849	20.190788281214054
6	18.93818520107764	37.651073070538445	25.346775390973182	18.063966337410736
7	14.9843306824856	26.63318496722945	39.89786307423965	18.484621276045296
8	17.404977114255473	26.48230872705078	31.75782879650954	24.354885362184206
9	18.052939695146414	24.877918754664677	32.892628328126044	24.176513222062866
10-14	19.1224576100282	30.82010518243859	27.450999980426538	22.606437227106678
15-19	19.322064632201812	29.152606138615106	28.402491245397343	23.122837983785743
20-24	19.356215558798155	29.309218621676525	28.06007718845124	23.274488631074085
25-29	19.532872073235914	29.354175984415853	27.866177679597286	23.246774262750947
30-34	19.39135847520504	29.311276072872367	27.82196625546814	23.475399196454454
35-39	19.491535471919622	29.115726364295586	28.01397052353453	23.378767640250263
40-44	19.465906264452602	29.20999769260376	27.92597802498183	23.398118017961814
45-49	19.63800132464767	29.00647176282089	27.946404307746796	23.409122604784635
50-54	19.606783636186574	29.116982217551136	27.908170746006007	23.36806340025629
55-59	19.682808589153453	29.016888768935594	27.86805311516673	23.43224952674423
60-64	19.677961977230733	28.950151512124258	27.80428693251987	23.567599578125144
65-69	19.66680345044266	28.81021032032262	27.993198303938705	23.529787925296013
70-74	19.74924024345498	28.812100094644784	27.962336543140015	23.47632311876022
75-79	19.601460555191622	28.825044202455675	27.982774523338538	23.590720719014165
80-84	19.844184111020905	28.665975750218315	27.822466915228983	23.667373223531797
85-89	19.787276851483597	28.726764559515	27.96947382153572	23.51648476746569
90-94	19.97657542998798	28.599043206455683	27.882627101114167	23.541754262442176
95-99	20.009159652269425	28.618384792501296	27.90486752892733	23.467588026301954
100-104	20.039601014066346	28.475740151339952	27.898708135444984	23.58595069914872
105-109	19.970867493603357	28.483552642360234	27.95062691519159	23.59495294884482
110-114	20.0399199026384	28.518579502525977	27.85355397192344	23.587946622912185
115-119	20.12567440094339	28.453034600849076	27.925437571039822	23.49585342716771
120-124	20.135290511639813	28.288132691257807	28.054658047781867	23.521918749320516
125-129	20.238662051866292	28.333051468468923	27.917050958382166	23.511235521282618
130-134	20.145047049900484	28.432095895533706	27.879030393276956	23.54382666128886
135-139	20.229884687381027	28.2891967464437	27.89402206903754	23.586896497137737
140-144	20.327359393418856	28.187842788090368	27.985609552777557	23.49918826571322
145-149	19.986924519796943	28.083115339909366	28.304163121700515	23.625797018593175
150	20.382837565355366	27.0563374596885	28.33705811437182	24.223766860584313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.5
6	1.5
7	4.0
8	9.0
9	13.0
10	29.5
11	53.5
12	90.5
13	170.0
14	298.0
15	446.5
16	815.0
17	1535.5
18	3293.0
19	6248.5
20	7767.0
21	9688.0
22	13985.5
23	19971.0
24	29407.0
25	41764.5
26	55919.5
27	75317.5
28	103417.0
29	140659.5
30	185142.5
31	237526.0
32	291431.5
33	349469.0
34	419984.5
35	503535.5
36	597985.0
37	704979.5
38	826795.5
39	962598.0
40	1104036.5
41	1242950.0
42	1377572.5
43	1489782.0
44	1571659.0
45	1607154.0
46	1565916.5
47	1473585.0
48	1336769.5
49	1164396.5
50	983954.5
51	808518.0
52	654072.5
53	528874.0
54	436125.5
55	345563.0
56	257877.0
57	181855.5
58	118465.0
59	76493.5
60	53131.0
61	40357.5
62	30417.5
63	20444.0
64	12591.5
65	8410.5
66	5613.0
67	3756.5
68	2394.0
69	1606.0
70	1644.5
71	2120.0
72	2260.0
73	1592.5
74	934.5
75	570.5
76	283.0
77	110.5
78	36.5
79	10.0
80	8.0
81	7.0
82	6.0
83	6.5
84	6.5
85	3.5
86	1.0
87	0.0
88	0.5
89	1.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.974046997412865E-4
3	6.057019872376932E-4
4	7.343099434319979E-4
5	6.720802872089472E-4
6	4.8124267479159185E-4
7	6.222965622305067E-4
8	7.260126559355912E-4
9	9.66633993331387E-4
10-14	8.347071221385195E-4
15-19	5.899371409945203E-4
20-24	5.766614810002696E-4
25-29	7.318207571830759E-4
30-34	3.775265810865074E-4
35-39	3.4848607484908376E-4
40-44	1.5432954743316567E-4
45-49	1.7507276617418255E-4
50-54	2.3896187989651455E-4
55-59	2.7712940237998565E-4
60-64	2.190483953576609E-4
65-69	1.6096738410825438E-4
70-74	1.0205665229487743E-4
75-79	1.9498635485488725E-4
80-84	5.4762171994192124E-5
85-89	2.173895925761421E-4
90-94	7.218686001836916E-5
95-99	5.8911418219376664E-5
100-104	7.052816911254347E-5
105-109	1.1201606260552846E-4
110-114	5.974241063945953E-5
115-119	1.2363485135356928E-4
120-124	1.4604020045278767E-4
125-129	1.0621264214062418E-4
130-134	9.459776086104277E-5
135-139	2.4729215915564036E-4
140-144	0.030020346404318984
145-149	8.503028597781465E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.0
72-73	2.0
74-75	4.0
76-77	4.0
78-79	9.0
80-81	9.0
82-83	11.0
84-85	15.0
86-87	27.0
88-89	33.0
90-91	28.0
92-93	55.0
94-95	46.0
96-97	57.0
98-99	58.0
100-101	58.0
102-103	54.0
104-105	65.0
106-107	68.0
108-109	89.0
110-111	79.0
112-113	102.0
114-115	100.0
116-117	107.0
118-119	100.0
120-121	124.0
122-123	128.0
124-125	144.0
126-127	174.0
128-129	196.0
130-131	248.0
132-133	303.0
134-135	353.0
136-137	602.0
138-139	1443.0
140-141	3257.0
142-143	5325.0
144-145	47324.0
146-147	604974.0
148-149	966917.0
150-151	2.2471567E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.14311725837532
#Duplication Level	Percentage of total
1	36.79422524776125
2	11.0404114453184
3	6.185242336392798
4	4.190050594227953
5	3.124023933159559
6	2.4896379480184585
7	2.066347273390904
8	1.6840331042507897
9	1.4432723718008553
>10	16.545473196623714
>50	4.68278506007336
>100	7.167359657286027
>500	1.4596100379590164
>1k	1.1063391692373237
>5k	0.021188624499567522
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	4.148643748203378E-6	1.7424303742454188E-4	1.5764846243172836E-4
2	0.0	4.148643748203378E-6	0.0	4.148643748203378E-6	0.0016719034305259614	3.6093200609369386E-4
3	0.0	4.148643748203378E-6	0.0	4.148643748203378E-6	0.0030119153611956524	4.687967435469817E-4
4	4.148643748203378E-6	4.148643748203378E-6	0.0	4.148643748203378E-6	0.004741899804196461	5.559182622592527E-4
5	4.148643748203378E-6	4.148643748203378E-6	0.0	4.148643748203378E-6	0.006563154409657744	6.222965622305067E-4
6	1.2445931244610134E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.007807747534118757	6.596343559643371E-4
7	1.2445931244610134E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.009168502683529466	6.80377574705354E-4
8	1.2445931244610134E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.010935824920264105	7.550531621730148E-4
9	2.074321874101689E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.014482915324977993	8.712151871227094E-4
10-11	2.074321874101689E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.018948930319918927	9.853028901983022E-4
12-13	2.074321874101689E-5	4.148643748203378E-6	0.0	4.148643748203378E-6	0.022568621990226377	0.0011056135588962002
14-15	2.074321874101689E-5	4.148643748203378E-6	0.0	6.222965622305067E-6	0.026547171344753415	0.0012487417682092168
16-17	2.074321874101689E-5	4.148643748203378E-6	0.0	1.86688968669152E-5	0.030206275130668793	0.001373201080655318
18-19	2.074321874101689E-5	4.148643748203378E-6	0.0	2.074321874101689E-5	0.0330356501669435	0.0014852144618568092
20-21	2.9040506237423647E-5	4.148643748203378E-6	0.0	2.074321874101689E-5	0.036163727553088845	0.0015847819118136905
22-23	3.73377937338304E-5	4.148643748203378E-6	0.0	2.074321874101689E-5	0.03911756190180965	0.0016760520742741647
24-25	3.73377937338304E-5	4.148643748203378E-6	0.0	2.074321874101689E-5	0.041998794984936895	0.0017963627429720627
26-27	3.73377937338304E-5	4.148643748203378E-6	0.0	2.4891862489220268E-5	0.04566412173647458	0.0019125247679217573
28-29	3.73377937338304E-5	4.148643748203378E-6	0.0	2.4891862489220268E-5	0.04938752950048711	0.0020058692522563334
30-31	4.5635081230237156E-5	4.148643748203378E-6	0.0	2.4891862489220268E-5	0.05327066004880547	0.0020763961959757906
32-33	4.9783724978440536E-5	4.148643748203378E-6	0.0	4.978372497844054E-5	0.056487933275537194	0.002169740680310367
34-35	4.9783724978440536E-5	4.148643748203378E-6	0.0	6.222965622305067E-5	0.05997071970215393	0.002300422958378773
36-37	4.9783724978440536E-5	4.148643748203378E-6	0.0	6.222965622305067E-5	0.06304279039769853	0.0023688755802241286
38-39	4.9783724978440536E-5	4.148643748203378E-6	0.0	6.637829997125405E-5	0.06604225982764958	0.0024331795583212813
40-41	4.9783724978440536E-5	4.148643748203378E-6	0.0	6.637829997125405E-5	0.06922012093877336	0.002534821330152264
42-43	4.9783724978440536E-5	4.148643748203378E-6	0.0	6.637829997125405E-5	0.07231500917493308	0.0025991253082494163
44-45	5.185804685254222E-5	4.148643748203378E-6	0.0	6.637829997125405E-5	0.07550946486104967	0.0026903954707098903
46-47	5.8081012474847295E-5	4.148643748203378E-6	0.0	6.637829997125405E-5	0.07863546792532093	0.0027920372425408735
48-49	5.8081012474847295E-5	6.222965622305067E-6	4.148643748203378E-6	6.637829997125405E-5	0.08306207080465394	0.0028646385081344326
50-51	7.260126559355911E-5	8.297287496406756E-6	4.148643748203378E-6	7.052694371945742E-5	0.0863519452969792	0.002928942486231585
52-53	7.46755874676608E-5	8.297287496406756E-6	4.148643748203378E-6	7.052694371945742E-5	0.08939082684253818	0.002995320786202839
54-55	7.882423121586418E-5	8.297287496406756E-6	4.148643748203378E-6	7.052694371945742E-5	0.09239029627248924	0.0030492531549294825
56-57	7.882423121586418E-5	8.297287496406756E-6	4.148643748203378E-6	7.260126559355911E-5	0.09543747510554461	0.003113557133026635
58-59	7.882423121586418E-5	8.297287496406756E-6	4.148643748203378E-6	7.674990934176249E-5	0.09787065466386588	0.003196530007990703
60-61	7.882423121586418E-5	8.297287496406756E-6	4.148643748203378E-6	7.882423121586418E-5	0.10039925302839585	0.0032774285610806687
62-63	7.882423121586418E-5	8.297287496406756E-6	4.148643748203378E-6	7.882423121586418E-5	0.10290918249605889	0.0033852932985339564
64-65	8.089855308996587E-5	8.297287496406756E-6	4.148643748203378E-6	7.882423121586418E-5	0.10569499677297746	0.0034827864266167357
66-67	8.297287496406757E-5	8.297287496406756E-6	4.148643748203378E-6	7.882423121586418E-5	0.10844969622178449	0.0035450160828397865
68-69	8.297287496406757E-5	8.297287496406756E-6	4.148643748203378E-6	8.089855308996587E-5	0.1107708623989043	0.003627988957803854
70-71	8.712151871227094E-5	8.297287496406756E-6	4.148643748203378E-6	8.297287496406757E-5	0.11320404195722557	0.0036715497171599895
72-73	9.54188062086777E-5	8.297287496406756E-6	4.148643748203378E-6	8.919584058637262E-5	0.11550446491560434	0.0037400023390053456
74-75	1.0371609370508444E-4	1.2445931244610134E-5	4.148643748203378E-6	9.127016246047431E-5	0.11804758353325301	0.0038437184327104294
76-77	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.12088733017889823	0.003910096732681684
78-79	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.12373744843391396	0.003966103423282429
80-81	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.1266290531264117	0.004001366895142158
82-83	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.12953102942827996	0.00404077901075009
84-85	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.13238529632704388	0.004107157310721345
86-87	1.0786473745328783E-4	1.6594574992813512E-5	4.148643748203378E-6	9.127016246047431E-5	0.13584111656929732	0.004225393657545141
88-89	1.1201338120149122E-4	2.2817540615118578E-5	4.148643748203378E-6	9.127016246047431E-5	0.13862900516808999	0.004312515176257412
90-91	1.1616202494969459E-4	2.4891862489220268E-5	4.148643748203378E-6	9.127016246047431E-5	0.14114100895762713	0.004376819154354564
92-93	1.1616202494969459E-4	2.4891862489220268E-5	4.148643748203378E-6	9.127016246047431E-5	0.14340409412227206	0.004443197454325818
94-95	1.1616202494969459E-4	2.4891862489220268E-5	4.148643748203378E-6	9.127016246047431E-5	0.14531869321206792	0.004528244651163986
96-97	1.1616202494969459E-4	2.4891862489220268E-5	4.148643748203378E-6	9.3344484334576E-5	0.14720217747375225	0.004578028376142428
98-99	1.1616202494969459E-4	2.4891862489220268E-5	4.148643748203378E-6	9.956744995688107E-5	0.14889689844489334	0.004611217526128054
100-101	1.1616202494969459E-4	2.9040506237423647E-5	4.148643748203378E-6	9.956744995688107E-5	0.15086750422528994	0.004644406676113681
102-103	1.1616202494969459E-4	2.9040506237423647E-5	4.148643748203378E-6	9.956744995688107E-5	0.15271987365886275	0.004696264722966224
104-105	1.1616202494969459E-4	2.9040506237423647E-5	4.148643748203378E-6	9.956744995688107E-5	0.15446437835498228	0.004754345735441071
106-107	1.1823634682379627E-4	2.9040506237423647E-5	4.148643748203378E-6	1.0579041557918614E-4	0.1563188221104292	0.004816575391664121
108-109	1.2031066869789796E-4	2.9040506237423647E-5	4.148643748203378E-6	1.1201338120149122E-4	0.158436704743887	0.004860136151020257
110-111	1.2031066869789796E-4	2.9040506237423647E-5	4.148643748203378E-6	1.1616202494969459E-4	0.16070808719602836	0.004934811738487917
112-113	1.2653363432020304E-4	2.9040506237423647E-5	4.148643748203378E-6	1.2031066869789796E-4	0.16229079478596795	0.005017784613451986
114-115	1.286079561943047E-4	2.9040506237423647E-5	4.148643748203378E-6	1.286079561943047E-4	0.16365984722287508	0.005050973763437612
116-117	1.327565999425081E-4	2.9040506237423647E-5	6.222965622305067E-6	1.286079561943047E-4	0.16523425752531823	0.005092460200919646
118-119	1.327565999425081E-4	2.9040506237423647E-5	8.297287496406756E-6	1.348309218166098E-4	0.16697876222143776	0.0051318723165275784
120-121	1.3897956556481315E-4	2.9040506237423647E-5	8.297287496406756E-6	1.3690524369071148E-4	0.1687398614925501	0.005200324938372934
122-123	1.4105388743891484E-4	2.9040506237423647E-5	8.297287496406756E-6	1.3897956556481315E-4	0.1710402844509289	0.005254257307099578
124-125	1.4520253118711823E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4105388743891484E-4	0.1725566137408972	0.005285372135211104
126-127	1.4520253118711823E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4105388743891484E-4	0.1742368144589196	0.005343453147685951
128-129	1.4935117493532159E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4105388743891484E-4	0.17607258931749958	0.0054036084820349
130-131	1.5349981868352497E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4520253118711823E-4	0.17770922927616578	0.005461689494509747
132-133	1.5972278430583005E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4520253118711823E-4	0.17914258569117006	0.005488655678873069
134-135	1.6179710617993175E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4520253118711823E-4	0.18074396217797656	0.005494878644495374
136-137	1.6179710617993175E-4	2.9040506237423647E-5	8.297287496406756E-6	1.4935117493532159E-4	0.1829904527676287	0.005540513725725612
138-139	1.6179710617993175E-4	3.111482811152534E-5	8.297287496406756E-6	1.5557414055762667E-4	0.184587680610687	0.005565405588214832
>>END_MODULE
