##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24104263
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	61-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.24349199143737	37.0	35.0	38.0	33.0	39.0
2	35.51117530538063	36.0	35.0	38.0	33.0	39.0
3	35.57649740214003	36.0	35.0	37.0	33.0	39.0
4	35.71767981456226	36.0	35.0	38.0	33.0	39.0
5	35.607645709806604	36.0	35.0	38.0	33.0	39.0
6	35.57554429272532	36.0	35.0	37.0	33.0	39.0
7	35.48508713998018	35.0	34.0	37.0	33.0	39.0
8	35.663458783203616	36.0	35.0	38.0	33.0	39.0
9	35.64443563364704	36.0	35.0	38.0	33.0	39.0
10-14	35.6135956116974	36.0	35.0	37.8	33.0	39.0
15-19	35.5659167094219	36.0	35.0	38.0	33.0	39.0
20-24	35.53426059946325	36.0	35.0	38.0	33.0	39.0
25-29	35.488796284706986	36.0	35.0	38.0	33.0	39.0
30-34	35.457734351803246	36.0	35.0	38.0	33.0	39.0
35-39	35.42286742390755	36.0	34.8	38.0	32.8	39.0
40-44	35.38242669356869	36.0	34.0	38.0	32.0	39.0
45-49	35.33547798578202	36.0	34.0	38.0	32.0	39.0
50-54	35.291849711397525	36.0	34.0	38.0	32.0	39.0
55-59	35.25634041580114	36.0	34.0	38.0	32.0	39.0
60-64	35.19794037335734	36.0	34.0	38.0	32.0	39.0
65-69	35.14290054028053	36.0	34.0	38.0	32.0	39.0
70-74	35.08063037825208	36.0	34.0	38.0	31.8	39.0
75-79	35.01782269698694	35.8	34.0	37.8	31.0	39.0
80-84	34.95248174565496	35.2	34.0	37.4	31.0	39.0
85-89	34.86449387089018	35.0	34.0	37.0	31.0	39.0
90-94	34.79073234253616	35.0	34.0	37.0	31.0	39.0
95-99	34.72727870288981	35.0	34.0	37.0	30.2	39.0
100-104	34.666790698379074	35.0	34.0	37.0	30.0	39.0
105-109	34.547730888790866	35.0	34.0	37.0	30.0	39.0
110-114	34.472391527754176	35.0	34.0	37.0	30.0	39.0
115-119	34.38027587542183	35.0	34.0	37.0	29.2	39.0
120-124	34.3151123809629	35.0	34.0	37.0	29.0	39.0
125-129	34.17322408007468	35.0	34.0	37.0	28.8	39.0
130-134	34.07935040695039	35.0	34.0	37.0	28.0	39.0
135-139	33.96536656718342	35.0	33.2	37.0	28.0	39.0
140-144	33.82646795845672	35.0	33.0	37.0	27.4	39.0
145-149	33.79753856607759	35.0	33.0	37.0	27.2	39.0
150	34.77013872261107	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	23.0
21	409.0
22	3127.0
23	15897.0
24	51491.0
25	114691.0
26	205914.0
27	323060.0
28	442265.0
29	550370.0
30	675804.0
31	849277.0
32	1099042.0
33	1507133.0
34	2271721.0
35	4000379.0
36	1.052105E7
37	1472607.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.190001992593594	20.1868067901516	17.338368735853905	28.284822481400905
2	23.958845950147737	25.443678798380137	33.21593085903232	17.38154439243981
3	20.532681254580627	28.762384851200075	31.82993153986231	18.875002354356987
4	23.926394112192764	32.95333356842522	24.053588504836323	19.066683814545687
5	25.04508767341872	35.128312873744335	23.472198479234827	16.354400973602115
6	19.85681122048826	38.87839258972573	23.855564470069048	17.409231719716963
7	20.439172937998563	21.61368717226492	38.913548196848005	19.033591692888514
8	22.179035301763843	23.79562071655126	30.214771553065116	23.810572428619782
9	23.340775861929487	23.282956214010774	29.923474532285017	23.452793391774723
10-14	22.810969163421426	29.62541605192409	26.82184060139072	20.741774183263765
15-19	22.802656940807527	28.390530753833875	28.405944624815948	20.40086768054265
20-24	22.735960893088894	28.57689162445684	28.155314712570405	20.531832769883856
25-29	22.83816438565936	28.523476937085867	28.073030222405592	20.56532845484918
30-34	22.79770180071467	28.344744661971205	28.305435432728228	20.552118104585897
35-39	22.900143431060307	28.393426507170123	28.10742149635523	20.59900856541434
40-44	23.117206280067553	28.09994647004972	28.16027853662234	20.62256871326039
45-49	23.14393433227973	28.04325193431552	28.170727310766562	20.642086422638187
50-54	23.172579057903576	28.042662826903275	28.138488200199273	20.646269914993876
55-59	23.300110855909594	27.845437132842438	28.176532922827803	20.67791908842017
60-64	23.142384721360767	27.791071369794036	28.2840630282235	20.7824808806217
65-69	23.14302510135327	27.865488574211934	28.322607882221885	20.66887844221291
70-74	23.36983796403931	27.781689401099964	28.220889498994815	20.627583135865915
75-79	23.229136952756217	27.782548966858045	28.31516716862268	20.673146911763062
80-84	23.384655314098488	27.859202145101698	28.09693498581482	20.65920755498499
85-89	23.374945069774714	27.813079922474838	28.221682553551414	20.590292454199037
90-94	23.344352511428422	27.828479723080722	28.220122043843944	20.607045721646912
95-99	23.25563821418656	27.943292526999365	28.277435076619312	20.523634182194762
100-104	23.48325869456778	27.877203239595406	28.112983698189264	20.526554367647552
105-109	23.246396163969866	27.95682987320435	28.3717155729235	20.425058389902286
110-114	23.354222915165842	27.932883770515843	28.24561712020835	20.467276194109964
115-119	23.420459132241003	27.919509752795346	28.33324224834598	20.326788866617676
120-124	23.48244142139767	27.92506982594198	28.36356430829942	20.228924444360928
125-129	23.41481185519906	28.156599955836707	28.187689571408725	20.240898617555512
130-134	23.582106039615145	28.10910121585602	28.148181700226548	20.160611044302286
135-139	23.371628127237198	28.1383097399798	28.36798234319151	20.122079789591492
140-144	23.346506133091395	28.205631428196742	28.229668900726722	20.218193537985137
145-149	23.184660197728657	28.029458139364095	28.4057881958524	20.380093467054845
150	23.754097642722375	26.926395347462755	28.496872130268798	20.822634879546072
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.5
6	1.0
7	2.5
8	10.5
9	18.0
10	27.5
11	48.0
12	73.0
13	102.0
14	174.5
15	308.0
16	457.5
17	708.5
18	1237.0
19	2088.0
20	3202.5
21	5018.0
22	7532.0
23	11142.5
24	16376.0
25	24222.5
26	42307.0
27	58919.5
28	72516.5
29	98857.5
30	131664.0
31	165645.5
32	212398.0
33	275132.0
34	348902.0
35	441102.5
36	559241.0
37	696689.0
38	839555.0
39	1003802.5
40	1188251.0
41	1359460.5
42	1511322.5
43	1609382.5
44	1642467.5
45	1655125.5
46	1613763.0
47	1489787.0
48	1327326.0
49	1139392.5
50	946974.5
51	780039.0
52	631583.5
53	529741.0
54	448158.0
55	353419.0
56	262442.0
57	184123.0
58	126022.0
59	81337.5
60	55774.5
61	43435.5
62	32249.0
63	21507.0
64	14563.0
65	9937.5
66	6584.0
67	4631.5
68	3538.0
69	2746.0
70	2044.5
71	1647.0
72	1361.0
73	1107.0
74	1163.5
75	1152.0
76	756.5
77	357.5
78	187.0
79	87.5
80	20.0
81	4.5
82	4.0
83	3.0
84	3.0
85	3.0
86	0.5
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.148643748203378E-6
3	7.052694371945743E-5
4	8.836611183673195E-4
5	0.13619167696602047
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	7.46755874676608E-6
25-29	0.05329015867442203
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	2.0
64-65	2.0
66-67	7.0
68-69	9.0
70-71	10.0
72-73	17.0
74-75	29.0
76-77	33.0
78-79	48.0
80-81	70.0
82-83	84.0
84-85	124.0
86-87	138.0
88-89	145.0
90-91	168.0
92-93	193.0
94-95	213.0
96-97	265.0
98-99	342.0
100-101	371.0
102-103	344.0
104-105	432.0
106-107	433.0
108-109	445.0
110-111	491.0
112-113	568.0
114-115	593.0
116-117	625.0
118-119	655.0
120-121	728.0
122-123	896.0
124-125	1005.0
126-127	1093.0
128-129	1270.0
130-131	1534.0
132-133	1895.0
134-135	2346.0
136-137	3300.0
138-139	5708.0
140-141	9159.0
142-143	13636.0
144-145	64704.0
146-147	735718.0
148-149	1173731.0
150-151	2.2080683E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.36512952919359
#Duplication Level	Percentage of total
1	27.615506433870696
2	10.644183416412679
3	6.791157738639872
4	4.595076980379059
5	3.3521413887859084
6	2.721076293270854
7	2.2549843437017474
8	1.8690197845739769
9	1.5211100554984731
>10	18.967311850528382
>50	5.811571906864079
>100	9.449503146471212
>500	2.1417053226613416
>1k	2.108102267544783
>5k	0.1121540056983641
>10k+	0.04539506509855013
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.6594574992813512E-5	0.0	0.0	0.0	0.0015225522555906397	0.0
2	3.73377937338304E-5	0.0	0.0	8.297287496406756E-6	0.010587338845415021	0.0
3	2.1987811865477902E-4	0.0	0.0	8.297287496406756E-6	0.012757079525725388	4.148643748203378E-6
4	3.4848607484908376E-4	0.0	0.0	1.2445931244610134E-5	0.01562379235573392	4.148643748203378E-6
5	3.775265810865074E-4	0.0	0.0	1.2445931244610134E-5	0.019183328691692418	4.148643748203378E-6
6	4.8124267479159185E-4	0.0	0.0	1.2445931244610134E-5	0.021427744959470446	8.297287496406756E-6
7	5.061345372808121E-4	0.0	0.0	1.6594574992813512E-5	0.024049687808334982	8.297287496406756E-6
8	5.559182622592527E-4	0.0	0.0	1.6594574992813512E-5	0.02941388417476195	8.297287496406756E-6
9	8.006882434032519E-4	0.0	0.0	2.074321874101689E-5	0.0410134920947386	8.297287496406756E-6
10-11	0.0010786473745328783	0.0	0.0	2.489186248922027E-5	0.05044128501253077	8.297287496406756E-6
12-13	0.001120133812014912	0.0	0.0	3.111482811152534E-5	0.05706874340028567	1.2445931244610134E-5
14-15	0.0012031066869789796	0.0	2.074321874101689E-6	3.3189149985627024E-5	0.07829527913796824	1.6594574992813512E-5
16-17	0.001246667446335115	0.0	4.148643748203378E-6	3.3189149985627024E-5	0.09647463604259546	1.6594574992813512E-5
18-19	0.0015142549680942329	0.0	4.148643748203378E-6	3.73377937338304E-5	0.10274945971175306	1.6594574992813512E-5
20-21	0.001833700536705893	0.0	4.148643748203378E-6	4.148643748203378E-5	0.11022324142414144	2.074321874101689E-5
22-23	0.0021552204271916547	0.0	4.148643748203378E-6	4.148643748203378E-5	0.11776755008024929	2.6966184363321958E-5
24-25	0.002182186611554977	0.0	4.148643748203378E-6	4.148643748203378E-5	0.12238913921574784	2.9040506237423647E-5
26-27	0.0022195244052888074	0.0	4.148643748203378E-6	6.015533434894898E-5	0.12665187066702682	3.73377937338304E-5
28-29	0.0024435511676917897	0.0	4.148643748203378E-6	6.222965622305067E-5	0.13140829072434201	4.148643748203378E-5
30-31	0.002733956230066026	6.222965622305067E-6	4.148643748203378E-6	6.222965622305067E-5	0.13465875310105935	4.9783724978440536E-5
32-33	0.0027650710581775513	1.2445931244610134E-5	4.148643748203378E-6	6.845262184535574E-5	0.13823695833388475	5.3932368726643915E-5
34-35	0.0028750101175049406	1.2445931244610134E-5	4.148643748203378E-6	7.052694371945742E-5	0.14153513011370644	5.600669060074561E-5
36-37	0.0029372397737279917	1.2445931244610134E-5	4.148643748203378E-6	7.46755874676608E-5	0.1436779046096535	6.222965622305067E-5
38-39	0.003040955867433076	1.2445931244610134E-5	4.148643748203378E-6	7.674990934176249E-5	0.14567547657441343	6.845262184535574E-5
40-41	0.003090739592411517	1.2445931244610134E-5	4.148643748203378E-6	1.0371609370508444E-4	0.14782447403598276	7.260126559355911E-5
42-43	0.003283651526702974	1.2445931244610134E-5	4.148643748203378E-6	1.0786473745328783E-4	0.14978885685075705	7.882423121586418E-5
44-45	0.003615543026559244	1.2445931244610134E-5	4.148643748203378E-6	1.0993905932738952E-4	0.15211209734975095	9.3344484334576E-5
46-47	0.004034556045127785	1.2445931244610134E-5	4.148643748203378E-6	1.2031066869789796E-4	0.15423205430508286	9.956744995688107E-5
48-49	0.004227467979419242	1.2445931244610134E-5	4.148643748203378E-6	1.2238499057199966E-4	0.15608234941678156	1.2031066869789796E-4
50-51	0.004694190401092122	1.4520253118711822E-5	4.148643748203378E-6	1.286079561943047E-4	0.1578372257222716	1.348309218166098E-4
52-53	0.004999115716585071	1.6594574992813512E-5	4.148643748203378E-6	1.327565999425081E-4	0.15975182481206746	1.638714280540334E-4
54-55	0.005279149169588798	1.6594574992813512E-5	4.148643748203378E-6	1.4105388743891484E-4	0.16129719460827324	1.9706057803966045E-4
56-57	0.005461689494509747	1.6594574992813512E-5	4.148643748203378E-6	1.4105388743891484E-4	0.16265380111393574	2.4062133739579593E-4
58-59	0.0055384394038515096	1.6594574992813512E-5	4.148643748203378E-6	1.4312820931301653E-4	0.16389839423839675	2.883307405001348E-4
60-61	0.00574794591313578	2.074321874101689E-5	4.148643748203378E-6	1.493511749353216E-4	0.16516373058159878	3.235942123598635E-4
62-63	0.006330830359758354	2.074321874101689E-5	4.148643748203378E-6	1.493511749353216E-4	0.16649129658102385	3.816752248347108E-4
64-65	0.0071232213156652	2.074321874101689E-5	4.148643748203378E-6	1.5349981868352497E-4	0.1675263831962006	4.169386966944395E-4
66-67	0.007681213899798554	2.074321874101689E-5	4.148643748203378E-6	1.5557414055762667E-4	0.16851790905202121	4.3560759356135465E-4
68-69	0.008185274115205265	2.074321874101689E-5	4.148643748203378E-6	1.6594574992813513E-4	0.16956751592031666	4.729453872951851E-4
70-71	0.008492273752572315	2.2817540615118578E-5	4.148643748203378E-6	1.7631735929864357E-4	0.17067312947921287	5.331007216441341E-4
72-73	0.009270144455360448	2.4891862489220268E-5	4.148643748203378E-6	1.9291193429145706E-4	0.1715816824600694	6.036276653635915E-4
74-75	0.0097036777270477	2.4891862489220268E-5	4.148643748203378E-6	2.0328354366196553E-4	0.17250683001591877	6.409654590974218E-4
76-77	0.01003556922690397	2.4891862489220268E-5	4.148643748203378E-6	2.0328354366196553E-4	0.17360622060919267	6.845262184535573E-4
78-79	0.010164177183098276	2.4891862489220268E-5	4.148643748203378E-6	2.0328354366196553E-4	0.17463300993687297	7.363842653060997E-4
80-81	0.010359163439263835	2.9040506237423647E-5	4.148643748203378E-6	2.1158083115837227E-4	0.17571165731140587	7.716477371658283E-4
82-83	0.010520960545443766	3.3189149985627024E-5	4.148643748203378E-6	2.1572947490657566E-4	0.17666999401724084	8.193571402701672E-4
84-85	0.010734615698476242	3.3189149985627024E-5	4.148643748203378E-6	2.1572947490657566E-4	0.17774449274802553	8.566949340039975E-4
86-87	0.01079684535469929	3.526347185972872E-5	4.148643748203378E-6	2.240267624029824E-4	0.1785638498882957	8.898840839896246E-4
88-89	0.01081966289531441	4.148643748203378E-5	4.148643748203378E-6	2.281754061511858E-4	0.17949522040976734	9.666339933313871E-4
90-91	0.010825885860936715	4.148643748203378E-5	4.148643748203378E-6	2.3024972802528749E-4	0.18050541516245489	0.0010018974651911157
92-93	0.010846629079677731	4.5635081230237156E-5	4.148643748203378E-6	2.3439837177349087E-4	0.18155294770887623	0.0010267893276803362
94-95	0.01085700068904824	4.5635081230237156E-5	4.148643748203378E-6	2.3854701552169423E-4	0.18244075747099175	0.0010496068682954544
96-97	0.01085700068904824	4.5635081230237156E-5	4.148643748203378E-6	2.447699811439993E-4	0.18344680357993107	0.001086944662029285
98-99	0.01085700068904824	4.5635081230237156E-5	4.148643748203378E-6	2.5099294676630436E-4	0.18468724806064388	0.001120133812014912
100-101	0.010865297976544647	4.5635081230237156E-5	4.148643748203378E-6	2.655131998850162E-4	0.18587376017263005	0.0011574716057487426
102-103	0.010865297976544647	4.5635081230237156E-5	4.148643748203378E-6	2.821077748778297E-4	0.18684039416596143	0.0012010323651048779
104-105	0.010877743907789257	4.5635081230237156E-5	4.148643748203378E-6	2.883307405001348E-4	0.1877945822280482	0.0012653363432020303
106-107	0.010890189839033866	4.5635081230237156E-5	4.148643748203378E-6	2.9455370612243984E-4	0.18866372309329682	0.0012923025275653522
108-109	0.010921304667145392	4.5635081230237156E-5	4.148643748203378E-6	2.987023498706432E-4	0.18969051242097715	0.0013711267587812165
110-111	0.010948270851508714	4.5635081230237156E-5	4.148643748203378E-6	3.13222602989355E-4	0.1908376124173554	0.0014167618400114535
112-113	0.010983534323368443	4.5635081230237156E-5	4.148643748203378E-6	3.194455686116601E-4	0.19187269903253212	0.0014769171743604025
114-115	0.011006351863983563	4.9783724978440536E-5	4.148643748203378E-6	3.256685342339652E-4	0.19281858980712252	0.001520477933716538
116-117	0.011020872117102273	4.9783724978440536E-5	4.148643748203378E-6	3.3189149985627026E-4	0.19385782506604743	0.0015661130149467752
118-119	0.011033318048346884	5.3932368726643915E-5	4.148643748203378E-6	3.360401436044736E-4	0.19473733754066658	0.001659457499281351
120-121	0.011043689657717392	5.3932368726643915E-5	4.148643748203378E-6	3.4433743110088037E-4	0.19572886339648718	0.0017175385117561984
122-123	0.011043689657717392	5.3932368726643915E-5	4.148643748203378E-6	3.4433743110088037E-4	0.19696515923345176	0.001794288421097961
124-125	0.011049912623339698	5.8081012474847295E-5	4.148643748203378E-6	3.464117529749821E-4	0.19806247550485157	0.0018627410429433167
126-127	0.0110519869452138	5.8081012474847295E-5	4.148643748203378E-6	3.567833623454905E-4	0.19916601474187368	0.0019374166304109773
128-129	0.0110519869452138	5.8081012474847295E-5	4.148643748203378E-6	3.6508064984189725E-4	0.2004251281194534	0.001999646286634028
130-131	0.011064432876458408	5.8081012474847295E-5	4.148643748203378E-6	3.6508064984189725E-4	0.20152451871272728	0.0020535786553606723
132-133	0.011068581520206613	6.015533434894898E-5	4.148643748203378E-6	3.6508064984189725E-4	0.20256582829352632	0.002086767805346299
134-135	0.011072730163954816	6.637829997125405E-5	4.148643748203378E-6	3.6508064984189725E-4	0.2034826785618793	0.0021324028865765364
136-137	0.011072730163954816	7.052694371945742E-5	4.148643748203378E-6	3.6508064984189725E-4	0.20465052177699855	0.00216144339281396
138-139	0.011072730163954816	7.260126559355911E-5	4.148643748203378E-6	3.6508064984189725E-4	0.20558811526409249	0.0022029298302959936
>>END_MODULE
