##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24019105
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	68-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.247574004110476	37.0	35.0	38.0	33.0	39.0
2	35.44617436827892	36.0	34.0	38.0	33.0	39.0
3	35.6563829085222	36.0	35.0	38.0	33.0	39.0
4	35.6420093504733	36.0	35.0	38.0	33.0	39.0
5	35.518722908284886	36.0	35.0	38.0	33.0	39.0
6	35.3970603817253	36.0	35.0	37.0	33.0	39.0
7	35.24991401636323	35.0	34.0	37.0	33.0	39.0
8	35.43748799133024	36.0	35.0	38.0	33.0	39.0
9	35.402554674705826	35.0	34.0	38.0	33.0	39.0
10-14	35.43334150876979	36.0	35.0	37.8	33.0	39.0
15-19	35.41704230028554	36.0	35.0	38.0	33.0	39.0
20-24	35.407767816494406	36.0	34.8	38.0	33.0	39.0
25-29	35.39291899510827	36.0	34.4	38.0	33.0	39.0
30-34	35.356323385072	36.0	34.0	38.0	32.4	39.0
35-39	35.31475614099693	36.0	34.0	38.0	32.0	39.0
40-44	35.28364265029859	36.0	34.0	38.0	32.0	39.0
45-49	35.2555306952528	36.0	34.0	38.0	32.0	39.0
50-54	35.21388089189834	36.0	34.0	37.8	32.0	39.0
55-59	35.16805459653888	36.0	34.0	37.6	32.0	39.0
60-64	35.13466500104813	36.0	34.0	37.0	32.0	39.0
65-69	35.07390546868096	36.0	34.0	37.0	32.0	39.0
70-74	35.04286582489182	36.0	34.0	37.0	32.0	39.0
75-79	34.9871411044792	35.8	34.0	37.0	31.8	39.0
80-84	34.94713107487848	35.4	34.0	37.0	31.0	39.0
85-89	34.886481493829855	35.0	34.0	37.0	31.0	39.0
90-94	34.83541037179074	35.0	34.0	37.0	31.0	39.0
95-99	34.767862282740786	35.0	34.0	37.0	31.0	39.0
100-104	34.71560156561867	35.0	34.0	37.0	31.0	39.0
105-109	34.65773214068217	35.0	34.0	37.0	30.2	39.0
110-114	34.59458785284338	35.0	34.0	37.0	30.0	39.0
115-119	34.535768849767166	35.0	34.0	37.0	30.0	39.0
120-124	34.47180047562333	35.0	34.0	37.0	30.0	39.0
125-129	34.3942555845306	35.0	34.0	37.0	30.0	39.0
130-134	34.334509587133816	35.0	34.0	37.0	29.8	39.0
135-139	34.27201938803255	35.0	34.0	37.0	29.0	39.0
140-144	34.20423995601479	35.0	34.0	37.0	29.0	39.0
145-149	34.19778139414408	35.0	34.0	37.0	29.0	39.0
150	35.000551942865776	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	76.0
23	966.0
24	6959.0
25	32098.0
26	98368.0
27	218261.0
28	363758.0
29	508905.0
30	677582.0
31	911484.0
32	1251322.0
33	1785763.0
34	2712836.0
35	4677198.0
36	9636013.0
37	1137492.0
38	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.302389285529166	12.274008544448263	15.170819229109494	36.25278294091308
2	22.418608400132527	12.538106287680062	34.00877355353899	31.034511758648424
3	21.324898947619715	16.29375669964989	27.36496834440798	35.01637600832242
4	24.539208298720013	23.37399417367957	22.437886812041153	29.64891071555926
5	24.598153043198774	30.35454059853524	24.401491820839944	20.645814537426045
6	19.230725034999367	37.443468726391096	25.34457504194803	17.98123119666151
7	15.148629550548536	26.604915411563663	39.58290840834183	18.66354662954597
8	17.318689627134226	26.841860171743452	31.65019391967972	24.1892562814426
9	17.894791009936043	25.13687440611914	32.92251686451975	24.04581771942507
10-14	19.06768071645463	30.795059681861886	27.559604527077568	22.577655074605918
15-19	19.309537293117916	29.132700409449036	28.426614877801658	23.131147419631386
20-24	19.355046985792963	29.277837308004678	28.0584126960442	23.30870301015816
25-29	19.543908121978333	29.261565451519573	27.866693807468916	23.32783261903318
30-34	19.390483934816345	29.25806893386531	27.78684961770998	23.564597513608362
35-39	19.47746126555025	29.04105485671909	28.028734595218317	23.452749282512343
40-44	19.440017868471358	29.134509574443747	27.922303424749266	23.503169132335636
45-49	19.678327646891315	28.900930325279074	27.941653873285823	23.47908815454379
50-54	19.601979259803716	29.050621748834438	27.891190721121166	23.456208270240676
55-59	19.709190636059716	28.918774168852917	27.845770983129466	23.5262642119579
60-64	19.688391152082442	28.835842638961502	27.787808612767524	23.687957596188532
65-69	19.680567042959463	28.67454940580111	27.986066233560663	23.658817317678768
70-74	19.74948826087134	28.684512983306515	27.943126366986505	23.62287238883564
75-79	19.57983169626617	28.71358465649967	27.96066066090071	23.745922986333447
80-84	19.8709164718831	28.525677628528573	27.775393235541575	23.828012664046753
85-89	19.804570717580713	28.567041085052168	27.96515156272757	23.66323663463955
90-94	20.020037717125124	28.422272127173432	27.869788129131855	23.68790202656959
95-99	20.051631473010882	28.48664615536702	27.864045342422493	23.597677029199605
100-104	20.081654347166992	28.291065884793603	27.872142387208694	23.75513738083071
105-109	19.9910023582275	28.325925937768755	27.94330008475956	23.739771619244188
110-114	20.089013455532417	28.36200243061171	27.80598080768368	23.743003306172188
115-119	20.17658299735713	28.273831984994285	27.894121649120006	23.655463368528572
120-124	20.175580066660356	28.077796434489	28.052166484024056	23.694457014826593
125-129	20.288941770269496	28.145386814970664	27.884064341464455	23.68160707329539
130-134	20.167005197242354	28.25055787945124	27.851663838992607	23.730773084313807
135-139	20.26840077469898	28.086076809024284	27.854806937724693	23.790715478552045
140-144	20.35046469021641	27.985759714857654	27.970364978655855	23.69341061627008
145-149	20.039998844188695	27.904041807925452	28.285364066198248	23.770595281687605
150	20.463307519527977	26.771940524447462	28.242950055960687	24.521801900063874
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	3.0
8	7.5
9	11.5
10	21.5
11	44.0
12	67.5
13	119.0
14	233.5
15	404.5
16	736.0
17	1494.0
18	3474.0
19	6977.0
20	8322.0
21	9458.0
22	13565.0
23	19743.5
24	29751.5
25	41909.5
26	55756.0
27	75703.5
28	104103.5
29	141388.5
30	186653.5
31	240423.5
32	294887.5
33	352123.5
34	419417.0
35	499597.5
36	590995.5
37	693378.5
38	807010.0
39	931571.0
40	1063734.0
41	1197808.0
42	1328581.5
43	1439376.5
44	1530993.5
45	1581148.5
46	1551418.5
47	1472070.0
48	1344099.0
49	1177411.0
50	1002500.5
51	833987.0
52	685981.5
53	561296.5
54	466618.5
55	373025.5
56	280262.0
57	196771.0
58	127126.5
59	79947.5
60	54231.5
61	41238.0
62	30822.5
63	20930.0
64	13226.5
65	8840.0
66	5988.5
67	4153.0
68	2748.0
69	1900.5
70	1981.0
71	2555.5
72	2701.0
73	1927.0
74	1145.0
75	670.0
76	355.5
77	139.0
78	35.0
79	10.5
80	6.0
81	7.5
82	6.0
83	4.0
84	2.0
85	1.0
86	1.5
87	1.0
88	0.5
89	0.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	1.0
96	1.5
97	0.5
98	1.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.288253038570755E-4
3	5.245824105436068E-4
4	7.160966239166697E-4
5	5.62052582725293E-4
6	4.121718939985482E-4
7	6.661363943410881E-4
8	5.82869345048452E-4
9	7.827102633507785E-4
10-14	6.911165091288789E-4
15-19	6.045187778645374E-4
20-24	5.045983187133743E-4
25-29	6.819571337066889E-4
30-34	3.713710398451566E-4
35-39	3.5638297097248214E-4
40-44	1.6070540513478748E-4
45-49	1.815221674579465E-4
50-54	2.714505806939934E-4
55-59	2.955980249888578E-4
60-64	2.356457494981599E-4
65-69	1.7819148696998898E-4
70-74	1.1241053526524577E-4
75-79	1.8485292177279148E-4
80-84	5.578901454018261E-5
85-89	1.8735164559943208E-4
90-94	6.74468294161436E-5
95-99	6.661454966349493E-5
100-104	4.996133409085818E-5
105-109	1.0991609829426953E-4
110-114	5.246065581965166E-5
115-119	1.2574084046676666E-4
120-124	1.3740126116370676E-4
125-129	8.410811690378701E-5
130-134	1.1658952617825021E-4
135-139	2.0571049159902088E-4
140-144	0.02358692056811047
145-149	6.928103905877416E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
68-69	1.0
70-71	4.0
72-73	1.0
74-75	1.0
76-77	4.0
78-79	6.0
80-81	23.0
82-83	22.0
84-85	26.0
86-87	24.0
88-89	32.0
90-91	41.0
92-93	43.0
94-95	67.0
96-97	63.0
98-99	87.0
100-101	83.0
102-103	95.0
104-105	112.0
106-107	93.0
108-109	139.0
110-111	132.0
112-113	147.0
114-115	134.0
116-117	137.0
118-119	163.0
120-121	163.0
122-123	215.0
124-125	210.0
126-127	255.0
128-129	295.0
130-131	391.0
132-133	464.0
134-135	594.0
136-137	867.0
138-139	1800.0
140-141	4225.0
142-143	6856.0
144-145	48296.0
146-147	584980.0
148-149	1055722.0
150-151	2.2312092E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.624119706830726
#Duplication Level	Percentage of total
1	33.669838388547916
2	10.294722228948025
3	5.981008046154834
4	4.017838928857674
5	3.145177618256757
6	2.5882949887243303
7	2.000803856423687
8	1.6906660368502473
9	1.5011396433784554
>10	17.739098441963357
>50	5.241669781831706
>100	8.35179701033284
>500	2.0229160572663987
>1k	1.7005447293179334
>5k	0.05448424314571847
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	8.3267049292636E-6	9.57571066865314E-5	1.2906392640358582E-4
2	0.0	4.1633524646318E-6	0.0	1.2490057393895401E-5	0.0012823125591065944	3.7053836935223023E-4
3	0.0	4.1633524646318E-6	0.0	1.2490057393895401E-5	0.002518828241102239	4.954389432911843E-4
4	8.3267049292636E-6	4.1633524646318E-6	0.0	1.66534098585272E-5	0.004088412120268428	5.82869345048452E-4
5	1.2490057393895401E-5	4.1633524646318E-6	0.0	2.0816762323159003E-5	0.0057953866307674665	6.245028696947701E-4
6	2.0816762323159003E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.007031902312763111	6.786264517349835E-4
7	2.0816762323159003E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.00828090805215265	7.160966239166697E-4
8	2.0816762323159003E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.010079476316873589	7.702202059568831E-4
9	2.0816762323159003E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.013197827312882807	8.451605503202555E-4
10-11	3.12251434847385E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.017615144277857146	9.700611242592094E-4
12-13	3.33068197170544E-5	4.1633524646318E-6	0.0	2.4980114787790802E-5	0.02111236034814786	0.0010845533170365839
14-15	3.33068197170544E-5	4.1633524646318E-6	0.0	2.91434672524226E-5	0.02500301322634628	0.0011719837187938518
16-17	3.33068197170544E-5	4.1633524646318E-6	0.0	4.9960229575581604E-5	0.028121364222355498	0.0012594141205511196
18-19	4.163352464631801E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.03084627841045701	0.0013197827312882806
20-21	4.9960229575581604E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.03367319473394201	0.0014259482191363916
22-23	6.036861073716111E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.03630859684405393	0.0015112969446613436
24-25	7.077699189874061E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.039004367564903025	0.0016299524899033497
26-27	7.077699189874061E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.04261607582797111	0.001709056186731354
28-29	7.077699189874061E-5	4.1633524646318E-6	0.0	6.66136394341088E-5	0.04621113068118067	0.0017402813302160925
30-31	7.077699189874061E-5	4.1633524646318E-6	0.0	7.28586681310565E-5	0.04997896466167245	0.0018443651418318875
32-33	7.077699189874061E-5	4.1633524646318E-6	0.0	8.951207798958371E-5	0.053157684268418826	0.0019692657157708415
34-35	7.077699189874061E-5	4.1633524646318E-6	0.0	9.15937542218996E-5	0.05635305728502374	0.0021087380233360065
36-37	7.077699189874061E-5	4.1633524646318E-6	0.0	9.15937542218996E-5	0.059371487821881785	0.0021711883103054837
38-39	7.077699189874061E-5	4.1633524646318E-6	0.0	9.15937542218996E-5	0.06217134235434668	0.002229475244810329
40-41	7.077699189874061E-5	4.1633524646318E-6	0.0	9.57571066865314E-5	0.065079444050892	0.002314823970335281
42-43	7.494034436337241E-5	4.1633524646318E-6	0.0	1.0408381161579501E-4	0.06771900951346854	0.0024105810770218127
44-45	7.494034436337241E-5	4.1633524646318E-6	0.0	1.0824716408042681E-4	0.07077491022250829	0.0024980114787790803
46-47	7.702202059568832E-5	4.1633524646318E-6	2.0816762323159E-6	1.1241051654505861E-4	0.0736163982796195	0.002606258642859507
48-49	7.910369682800421E-5	4.1633524646318E-6	4.1633524646318E-6	1.1449219277737452E-4	0.07797126495762435	0.00271658748317225
50-51	8.534872552495191E-5	4.1633524646318E-6	4.1633524646318E-6	1.1657386900969042E-4	0.0810521457814519	0.0027769560939094108
52-53	8.951207798958371E-5	4.1633524646318E-6	4.1633524646318E-6	1.1657386900969042E-4	0.0838145301417351	0.002856059790737415
54-55	9.15937542218996E-5	4.1633524646318E-6	4.1633524646318E-6	1.1865554524200631E-4	0.08653319930113965	0.002918510077706892
56-57	9.57571066865314E-5	4.1633524646318E-6	4.1633524646318E-6	1.2698225017126991E-4	0.0893913407681094	0.0029497352211916303
58-59	9.57571066865314E-5	4.1633524646318E-6	4.1633524646318E-6	1.332272788682176E-4	0.09171240976714162	0.0030205122130903712
60-61	9.57571066865314E-5	4.1633524646318E-6	4.1633524646318E-6	1.332272788682176E-4	0.09424164638940544	0.0031141876435445866
62-63	9.57571066865314E-5	4.1633524646318E-6	4.1633524646318E-6	1.332272788682176E-4	0.0967521479255784	0.0031849646354433275
64-65	9.783878291884731E-5	4.1633524646318E-6	4.1633524646318E-6	1.353089551005335E-4	0.09953326737195245	0.0032453332461804887
66-67	1.020021353834791E-4	4.1633524646318E-6	4.1633524646318E-6	1.373906313328494E-4	0.10244969577342702	0.003336927000402388
68-69	1.0408381161579501E-4	8.3267049292636E-6	4.1633524646318E-6	1.394723075651653E-4	0.10456051547299536	0.003413949020998076
70-71	1.0616548784811092E-4	8.3267049292636E-6	4.1633524646318E-6	1.4155398379748122E-4	0.106752520545624	0.003447255840715131
72-73	1.0824716408042681E-4	8.3267049292636E-6	4.1633524646318E-6	1.4155398379748122E-4	0.10896326070434348	0.003513869480149239
74-75	1.1032884031274271E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.1114279653634055	0.0036012998819065075
76-77	1.165738690096904E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.11413414446541617	0.0036720768738052476
78-79	1.228188977066381E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.1169922859323859	0.0037199554271485137
80-81	1.24900573938954E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.11980254884601238	0.0037678339804917793
82-83	1.2906392640358582E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.12255452482513399	0.003799059123976518
84-85	1.394723075651653E-4	1.2490057393895401E-5	4.1633524646318E-6	1.4155398379748122E-4	0.12518992693524592	0.0038719177921075747
86-87	1.4155398379748122E-4	2.0816762323159003E-5	4.1633524646318E-6	1.4155398379748122E-4	0.1286184476898702	0.0039218780216831554
88-89	1.4363566002979712E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.13127050320984068	0.0039843283086526335
90-91	1.45717336262113E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.13349581510218636	0.004032206861995898
92-93	1.477990124944289E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.13541303891214929	0.0041279639686824295
94-95	1.4988068872674482E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.13721577052933487	0.004182087550722644
96-97	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.1390393189088436	0.004229966104065909
98-99	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.14053812579611105	0.004277844657409175
100-101	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.14248865642579106	0.004329886563217073
102-103	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.45717336262113E-4	0.1440915471246743	0.004373601764095706
104-105	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.477990124944289E-4	0.1456111707742649	0.004431888698600551
106-107	1.540440411913766E-4	2.0816762323159003E-5	4.1633524646318E-6	1.5404404119137664E-4	0.14736394216187487	0.004490175633105397
108-109	1.5820739365600842E-4	2.2898438555474904E-5	4.1633524646318E-6	1.7069745104990382E-4	0.14935818799243353	0.004523482452822451
110-111	1.5820739365600842E-4	2.4980114787790802E-5	4.1633524646318E-6	1.748608035145356E-4	0.15130871862211354	0.004598422797185824
112-113	1.5820739365600842E-4	2.4980114787790802E-5	4.1633524646318E-6	1.748608035145356E-4	0.15295948787434005	0.004677526494013828
114-115	1.5820739365600842E-4	2.4980114787790802E-5	4.1633524646318E-6	1.7902415597916742E-4	0.1544083345320319	0.004737895104750989
116-117	1.5820739365600842E-4	2.4980114787790802E-5	4.1633524646318E-6	1.7902415597916742E-4	0.15574060732071407	0.004771201924468043
118-119	1.623707461206402E-4	2.4980114787790802E-5	4.1633524646318E-6	1.8943253714074693E-4	0.15735806975322353	0.004810753772882046
120-121	1.623707461206402E-4	2.4980114787790802E-5	4.1633524646318E-6	2.0192259453464232E-4	0.15913373957938898	0.004871122383619206
122-123	1.623707461206402E-4	2.4980114787790802E-5	4.1633524646318E-6	2.0400427076695823E-4	0.1614256651111688	0.004914837584497841
124-125	1.623707461206402E-4	2.4980114787790802E-5	4.1633524646318E-6	2.0608594699927414E-4	0.16275793789985096	0.004952307756679527
126-127	1.6445242235295612E-4	2.4980114787790802E-5	4.1633524646318E-6	2.1857600439316953E-4	0.16411935415578555	0.004981451223931949
128-129	1.686157748175879E-4	2.4980114787790802E-5	4.1633524646318E-6	2.2482103309011723E-4	0.16571808150220418	0.005004349662487424
130-131	1.7069745104990382E-4	2.4980114787790802E-5	4.1633524646318E-6	2.3314773801938083E-4	0.16727309364774418	0.005045983187133742
132-133	1.769424797468515E-4	2.9143467252422604E-5	6.2450286969477005E-6	2.3314773801938083E-4	0.16839303546073012	0.00509802509294164
134-135	1.7902415597916742E-4	2.9143467252422604E-5	1.0408381161579502E-5	2.3314773801938083E-4	0.1697919218888464	0.005114678502800167
136-137	1.7902415597916742E-4	3.12251434847385E-5	1.2490057393895401E-5	2.3314773801938083E-4	0.17220666631833284	0.005141740293820273
138-139	1.7902415597916742E-4	3.33068197170544E-5	1.2490057393895401E-5	2.3731109048401262E-4	0.17384286383693315	0.005164638732375749
>>END_MODULE
