##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	mock.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24019105
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	63-150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.19302122206469	37.0	35.0	38.0	33.0	39.0
2	35.41377603370317	35.0	34.0	37.0	33.0	39.0
3	35.5122232489512	36.0	35.0	37.0	33.0	39.0
4	35.62157232752844	36.0	35.0	38.0	33.0	39.0
5	35.53092390411716	36.0	35.0	38.0	33.0	39.0
6	35.49748939438002	36.0	35.0	37.0	33.0	39.0
7	35.409074193230765	35.0	34.0	37.0	33.0	39.0
8	35.57099088413161	36.0	35.0	38.0	33.0	39.0
9	35.55372766803759	36.0	35.0	38.0	33.0	39.0
10-14	35.530608388613985	36.0	35.0	37.8	33.0	39.0
15-19	35.490235243985985	36.0	35.0	38.0	33.0	39.0
20-24	35.46475077235392	36.0	35.0	38.0	33.0	39.0
25-29	35.43172408797081	36.0	35.0	38.0	33.0	39.0
30-34	35.4014485135895	36.0	34.0	38.0	32.8	39.0
35-39	35.37158427843168	36.0	34.0	38.0	32.2	39.0
40-44	35.33474595327344	36.0	34.0	38.0	32.0	39.0
45-49	35.29642016220005	36.0	34.0	38.0	32.0	39.0
50-54	35.25614186706791	36.0	34.0	38.0	32.0	39.0
55-59	35.22020276775508	36.0	34.0	38.0	32.0	39.0
60-64	35.17513786054929	36.0	34.0	38.0	32.0	39.0
65-69	35.121485213457774	36.0	34.0	37.8	32.0	39.0
70-74	35.06321379815011	35.8	34.0	37.6	31.8	39.0
75-79	35.00665961802518	35.2	34.0	37.2	31.0	39.0
80-84	34.94467737372337	35.2	34.0	37.2	31.0	39.0
85-89	34.859453914479	35.0	34.0	37.0	31.0	39.0
90-94	34.79131358676234	35.0	34.0	37.0	31.0	39.0
95-99	34.726759077971465	35.0	34.0	37.0	30.2	39.0
100-104	34.677723988668426	35.0	34.0	37.0	30.0	39.0
105-109	34.56149106529661	35.0	34.0	37.0	30.0	39.0
110-114	34.48952280251423	35.0	34.0	37.0	30.0	39.0
115-119	34.40685457975453	35.0	34.0	37.0	29.8	39.0
120-124	34.344063752811024	35.0	34.0	37.0	29.0	39.0
125-129	34.20652526062931	35.0	34.0	37.0	29.0	39.0
130-134	34.11845717906609	35.0	34.0	37.0	28.6	39.0
135-139	34.00462094406576	35.0	33.8	37.0	28.0	39.0
140-144	33.869971120520376	35.0	33.0	37.0	28.0	39.0
145-149	33.846838450100414	35.0	33.0	37.0	27.8	39.0
150	34.804217585464826	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	45.0
21	498.0
22	3998.0
23	18985.0
24	58762.0
25	127128.0
26	219950.0
27	333804.0
28	451715.0
29	558038.0
30	683632.0
31	849342.0
32	1092779.0
33	1481793.0
34	2204429.0
35	3862203.0
36	1.0527916E7
37	1544083.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.70639726167982	20.419478577573976	16.87726915719799	27.996855003548216
2	23.538326972493522	25.974812631429238	32.849045195401345	17.637815200675895
3	20.62470984013672	29.159168289785537	31.147262175285846	19.068859694791897
4	23.96712189698581	33.08469445566708	23.837969459389154	19.11021418795795
5	24.97976258067721	35.091949756102785	23.446186510840516	16.48210115237948
6	20.15339039485443	38.720922365758426	23.81285231069184	17.312834928695302
7	20.65794291669069	21.789729467438523	38.4762796115842	19.076048004286587
8	22.403120349405192	23.97931979563768	29.916098039456507	23.70146181550062
9	23.419490443128502	23.39740385830363	29.764976671695305	23.418129026872567
10-14	23.00293620432568	29.58092318593886	26.607936473902754	20.808204135832707
15-19	22.941265296937583	28.420989041848145	28.227224952803198	20.410520708411077
20-24	22.859076318429917	28.61324862297089	27.971635073753802	20.556039984845395
25-29	22.95669957099421	28.541567745428797	27.89898454746137	20.602748136115622
30-34	22.940113713645864	28.3597827645951	28.115218281447206	20.584885240311827
35-39	23.057571878719045	28.419811645771148	27.909634434755166	20.61298204075464
40-44	23.263533757814873	28.12406707077553	27.958065048635245	20.65433412277435
45-49	23.326062315810685	28.05016673185783	27.96108597718358	20.662684975147908
50-54	23.325033135081426	28.039660928248576	27.950362013905185	20.684943922764816
55-59	23.46473192902067	27.843596170631667	27.98426419302468	20.707407707322982
60-64	23.297268125473753	27.764487940154105	28.100981749336317	20.837262185035822
65-69	23.283922788604418	27.838367112635414	28.17047951469616	20.707230584064003
70-74	23.51227302592186	27.749781960699522	28.076101915110396	20.661843098268225
75-79	23.366266946277452	27.749414915436176	28.177251926813657	20.70706621147271
80-84	23.532232683801578	27.820787152875347	27.96153314744783	20.68544701587524
85-89	23.516321583585317	27.7738930620335	28.095863946685988	20.613921407695194
90-94	23.492864540915107	27.794196388579557	28.08608415982492	20.62685491068042
95-99	23.40341017134668	27.93057035428982	28.136723410250625	20.529296064112877
100-104	23.63037330685308	27.855956876350064	27.965405680527095	20.54826413626976
105-109	23.3979834049098	27.93376131174442	28.235722342920266	20.43253294042551
110-114	23.50264343813905	27.885360420071887	28.133091187718378	20.47890495407069
115-119	23.566430195379358	27.896493427791256	28.21267547598544	20.32440090084395
120-124	23.641294787339223	27.89966108103044	28.26029680514082	20.198747326489517
125-129	23.54890804039302	28.15767993619601	28.067818962308174	20.2255930611028
130-134	23.729430196394173	28.111668986924215	28.034889333166717	20.124011483514895
135-139	23.501766115318958	28.154425324861688	28.266307516996658	20.077501042822696
140-144	23.439967663705204	28.25132996521947	28.11671270908284	20.191989661992487
145-149	23.31919512961109	28.11830741455742	28.225430388399452	20.337067067432045
150	23.896096780610986	27.0476715480779	28.30931092284572	20.74692074846539
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	1.5
2	0.5
3	0.5
4	0.5
5	0.5
6	1.5
7	6.5
8	11.0
9	13.5
10	22.0
11	32.0
12	55.0
13	87.0
14	141.0
15	239.5
16	406.0
17	703.0
18	1193.5
19	1902.5
20	2905.0
21	4443.5
22	6652.5
23	10134.0
24	14814.5
25	21780.0
26	41180.5
27	57061.0
28	67173.0
29	92865.5
30	125767.5
31	157253.0
32	201373.5
33	262956.5
34	334738.0
35	424058.0
36	538489.0
37	672694.0
38	810566.0
39	972551.5
40	1158279.0
41	1329421.5
42	1482361.0
43	1582824.0
44	1624973.0
45	1649784.5
46	1618100.5
47	1502906.0
48	1348678.5
49	1162742.0
50	969519.0
51	804270.0
52	655033.5
53	555597.0
54	476068.0
55	376470.5
56	278455.5
57	193758.5
58	130954.5
59	83601.5
60	56696.5
61	44403.5
62	33294.0
63	22272.0
64	15051.0
65	9959.0
66	6472.5
67	4729.0
68	3642.5
69	2802.0
70	2254.0
71	1983.0
72	1740.5
73	1438.0
74	1416.0
75	1375.5
76	948.5
77	421.0
78	186.0
79	95.0
80	27.0
81	8.5
82	4.0
83	4.0
84	5.5
85	4.5
86	3.5
87	2.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.3267049292636E-6
3	7.494034436337241E-5
4	8.368338453909918E-4
5	0.10485819517421652
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	2.0816762323159003E-5
25-29	0.04207067665510434
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	4.16349661503978E-6
95-99	0.007422018406455759
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
62-63	2.0
64-65	2.0
66-67	6.0
68-69	13.0
70-71	16.0
72-73	22.0
74-75	22.0
76-77	29.0
78-79	50.0
80-81	65.0
82-83	88.0
84-85	92.0
86-87	125.0
88-89	127.0
90-91	176.0
92-93	167.0
94-95	227.0
96-97	281.0
98-99	337.0
100-101	360.0
102-103	381.0
104-105	373.0
106-107	379.0
108-109	437.0
110-111	479.0
112-113	458.0
114-115	527.0
116-117	571.0
118-119	666.0
120-121	735.0
122-123	805.0
124-125	981.0
126-127	1116.0
128-129	1240.0
130-131	1573.0
132-133	1902.0
134-135	2284.0
136-137	3102.0
138-139	5665.0
140-141	9267.0
142-143	13453.0
144-145	64178.0
146-147	733503.0
148-149	1161224.0
150-151	2.2011599E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.3148579596719
#Duplication Level	Percentage of total
1	26.094313679172593
2	9.938870771732509
3	6.39318788784064
4	4.400493650291757
5	3.321508543589754
6	2.71727750608495
7	2.135577636219439
8	1.7558013135755477
9	1.6222501269454588
>10	19.554485536274953
>50	5.998901921418167
>100	10.573028615023055
>500	2.453406826728026
>1k	2.594205106981553
>5k	0.34385837264089886
>10k+	0.10283250548065084
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.4980114787790802E-5	4.1633524646318E-6	0.0	0.0	0.0020483694125988457	0.0
2	6.66136394341088E-5	4.1633524646318E-6	0.0	0.0	0.012215276131229703	0.0
3	2.0400427076695823E-4	8.3267049292636E-6	0.0	4.1633524646318E-6	0.014580060331140565	0.0
4	3.6221166442296666E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.017727554794402207	0.0
5	4.0384518906928465E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.021532858947075673	0.0
6	4.7045882850339343E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.0240766673029657	0.0
7	5.370724679375022E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.027003504085601857	0.0
8	5.995227549069793E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.03276142054418764	0.0
9	8.867940749665735E-4	8.3267049292636E-6	0.0	1.66534098585272E-5	0.044830979339155225	8.3267049292636E-6
10-11	0.0012635774730157515	8.3267049292636E-6	0.0	1.66534098585272E-5	0.0535469577238619	1.2490057393895401E-5
12-13	0.0012968842927328058	8.3267049292636E-6	0.0	2.0816762323159003E-5	0.0598044764782035	1.66534098585272E-5
14-15	0.001373906313328494	8.3267049292636E-6	0.0	2.4980114787790802E-5	0.08066079064977649	1.66534098585272E-5
16-17	0.001415539837974812	8.3267049292636E-6	0.0	2.91434672524226E-5	0.09885672259644979	1.66534098585272E-5
18-19	0.0015945639939539797	8.3267049292636E-6	0.0	3.33068197170544E-5	0.10546604463405276	1.66534098585272E-5
20-21	0.001898488723872101	8.3267049292636E-6	0.0	3.33068197170544E-5	0.11359707199747868	1.66534098585272E-5
22-23	0.0022440469784365405	8.3267049292636E-6	0.0	3.33068197170544E-5	0.1220465958244489	1.66534098585272E-5
24-25	0.0022752721219212787	8.3267049292636E-6	0.0	3.9551848414002106E-5	0.12690522815067423	1.66534098585272E-5
26-27	0.0023044155891737018	8.3267049292636E-6	0.0	4.9960229575581604E-5	0.13144536401335521	2.4980114787790802E-5
28-29	0.002545890032122346	8.3267049292636E-6	0.0	4.9960229575581604E-5	0.13616452403201534	2.4980114787790802E-5
30-31	0.0028060995611618334	8.3267049292636E-6	0.0	5.4123582040213406E-5	0.13943691906921596	2.4980114787790802E-5
32-33	0.002849814762040467	8.3267049292636E-6	0.0	5.4123582040213406E-5	0.14308817918069805	2.7061791020106703E-5
34-35	0.0029330818113331033	8.3267049292636E-6	0.0	5.620525827252931E-5	0.1462814705210706	3.33068197170544E-5
36-37	0.0029767970122117375	8.3267049292636E-6	0.0	6.45319632017929E-5	0.14831943155250787	4.163352464631801E-5
38-39	0.0030850441762921644	8.3267049292636E-6	0.0	7.494034436337241E-5	0.15028869726827873	4.78785533432657E-5
40-41	0.0031225143484738503	1.0408381161579502E-5	0.0	8.74304017572678E-5	0.15241408870147327	6.036861073716111E-5
42-43	0.0032807217421298588	1.2490057393895401E-5	0.0	9.15937542218996E-5	0.15441874291319346	9.15937542218996E-5
44-45	0.0035388495949370304	1.66534098585272E-5	0.0	9.783878291884731E-5	0.15660450295712516	9.992045915116321E-5
46-47	0.003896897906895365	1.66534098585272E-5	0.0	1.1241051654505861E-4	0.15857376867289602	1.0824716408042681E-4
48-49	0.004073840386642217	1.66534098585272E-5	0.0	1.1865554524200631E-4	0.16031613167934441	1.165738690096904E-4
50-51	0.0044735222232468695	1.66534098585272E-5	0.0	1.24900573938954E-4	0.16232078589106463	1.3739063133284943E-4
52-53	0.00479826371548815	1.66534098585272E-5	0.0	1.353089551005335E-4	0.16417347773782578	1.602890698883243E-4
54-55	0.005106351797870903	1.66534098585272E-5	0.0	1.4155398379748122E-4	0.16566187624393164	1.9984091830232642E-4
56-57	0.0053394995358902846	1.66534098585272E-5	0.0	1.4155398379748122E-4	0.16694627047927058	2.393927667163285E-4
58-59	0.0054331749663445	1.66534098585272E-5	0.0	1.4155398379748122E-4	0.16806829396848882	2.643728815041193E-4
60-61	0.005637179237111458	1.66534098585272E-5	0.0	1.4363566002979712E-4	0.16914452058059615	3.039247299181214E-4
62-63	0.006203395172301383	1.66534098585272E-5	0.0	1.477990124944289E-4	0.17026446239358212	3.5180328326138714E-4
64-65	0.006994432140581425	1.66534098585272E-5	0.0	1.540440411913766E-4	0.1711803999358011	3.788650742814938E-4
66-67	0.007658486858690196	1.66534098585272E-5	0.0	1.540440411913766E-4	0.1722004212896359	3.9343680790770514E-4
68-69	0.008208049384021594	1.66534098585272E-5	0.0	1.5820739365600842E-4	0.17316215570896584	4.2258027516012774E-4
70-71	0.008514055790172032	1.66534098585272E-5	0.0	1.623707461206402E-4	0.1741800953865683	4.99602295755816E-4
72-73	0.009436238361087975	1.66534098585272E-5	0.0	1.7069745104990382E-4	0.1750689711377672	5.453991728667658E-4
74-75	0.010019107706136426	1.66534098585272E-5	0.0	1.7069745104990382E-4	0.1759620102414307	5.620525827252931E-4
76-77	0.010375074341862446	1.66534098585272E-5	0.0	1.7069745104990382E-4	0.17688211113611435	5.932777262100315E-4
78-79	0.010524955030589192	1.66534098585272E-5	0.0	1.7277912728221972E-4	0.17782927382181807	6.203395172301382E-4
80-81	0.01073936768251773	1.66534098585272E-5	0.0	1.811058322114833E-4	0.17870357783939078	6.45319632017929E-4
82-83	0.010893411723709105	1.66534098585272E-5	0.0	1.831875084437992E-4	0.17962784208653904	6.86953156664247E-4
84-85	0.011153621252748594	1.66534098585272E-5	0.0	1.831875084437992E-4	0.18042512408351602	7.223416526136174E-4
86-87	0.011238969978273545	1.66534098585272E-5	0.0	1.8943253714074693E-4	0.18121407937556375	7.577301485629877E-4
88-89	0.011274358474222916	1.66534098585272E-5	0.0	1.915142133730628E-4	0.18221744731954	8.076903781385693E-4
90-91	0.011278521826687548	1.66534098585272E-5	0.0	1.9567756583769463E-4	0.18317710006263765	8.34752169158676E-4
92-93	0.011288930207849126	1.66534098585272E-5	0.0	2.0400427076695823E-4	0.18408262922369506	8.805490462696257E-4
94-95	0.01129725691277839	1.66534098585272E-5	0.0	2.0400427076695823E-4	0.18502771023316647	8.992841323604689E-4
96-97	0.011299338589010706	1.66534098585272E-5	0.0	2.0400427076695823E-4	0.1860685483493244	9.201008946836278E-4
98-99	0.011309746970172285	1.8735086090843102E-5	0.0	2.0608594699927414E-4	0.18724053206811828	9.534077144006822E-4
100-101	0.011313910322636917	2.0816762323159003E-5	0.0	2.123309756962218E-4	0.18853117133215413	0.0010096129726732115
102-103	0.011328482056263129	2.0816762323159003E-5	0.0	2.123309756962218E-4	0.1895366209523627	0.0010325114112286864
104-105	0.011336808761192392	2.0816762323159003E-5	0.0	2.2482103309011723E-4	0.19050876375285425	0.001103288403127427
106-107	0.011355543847283236	2.0816762323159003E-5	0.0	2.3314773801938083E-4	0.19141221123767932	0.0011407585753091132
108-109	0.01137427893337408	2.2898438555474904E-5	0.0	2.4355611918096032E-4	0.19229276028394895	0.0011928004811170108
110-111	0.011413830781788082	2.4980114787790802E-5	0.0	2.70617910201067E-4	0.19353552099464155	0.001234434005763329
112-113	0.011457545982666715	2.4980114787790802E-5	0.0	2.747812626656988E-4	0.19452431720499164	0.001271904177945015
114-115	0.011476281068757558	2.4980114787790802E-5	0.0	2.789446151303306E-4	0.195390294517635	0.0013010476451974376
116-117	0.011488771126151454	2.4980114787790802E-5	0.0	2.789446151303306E-4	0.19649774627322708	0.0013301911124498603
118-119	0.011513751240939243	2.4980114787790802E-5	0.0	2.8727132005959425E-4	0.197544829418082	0.001394723075651653
120-121	0.011522077945868507	2.9143467252422604E-5	0.0	2.8727132005959425E-4	0.19863146441135088	0.0014280298953687075
122-123	0.011524159622100823	2.9143467252422604E-5	0.0	2.893529962919101E-4	0.19981593818753862	0.0014863168298735528
124-125	0.011530404650797771	3.33068197170544E-5	0.0	2.91434672524226E-4	0.20083595954137343	0.0015321137069845025
126-127	0.011532486327030087	3.33068197170544E-5	0.0	2.91434672524226E-4	0.2018538992189759	0.0015987273464186113
128-129	0.01154081303195935	3.33068197170544E-5	0.0	2.976797012211737E-4	0.20301547455660818	0.0016549326046911405
130-131	0.011542894708191666	3.5388495949370304E-5	0.0	2.9976137745348964E-4	0.20415206977945266	0.001690321100640511
132-133	0.011549139736888614	3.7470172181686205E-5	0.0	2.9976137745348964E-4	0.2053490336130343	0.0017839965310947265
134-135	0.011549139736888614	3.7470172181686205E-5	0.0	2.9976137745348964E-4	0.20634199317584898	0.0018464468180642035
136-137	0.011549139736888614	3.7470172181686205E-5	0.0	3.0392472991812145E-4	0.20744736325520874	0.0019151421337306282
138-139	0.011549139736888614	3.7470172181686205E-5	0.0	3.0600640615043734E-4	0.2084132610270033	0.0019692657157708415
>>END_MODULE
