FastQCFastQC Report
Mon 6 Apr 2020
Mlat_P_5A.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_P_5A.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37649935
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGT38600.017.4954721
GTCCGCT58000.015.1020151
CGCGGTA75750.013.1359474
GCGGGAA140700.012.8785441
CCCGAAT181550.012.0064041
CCCGTGT83600.011.6771851
CGCGCTA34050.011.1793224
GCCCTAT75650.011.1366151
GTCCGTA115200.011.0723214
CCCGTTT144050.010.8151921
CCCGACT165850.010.8046551
CCGACAT187100.010.7210931
AGACGTG113600.010.6403458
CTCCTAT207150.010.4903511
GTCCGAA132150.010.3723761
GTCCAGT252200.010.2602391
CTCGTAT150050.010.2044841
GCCTGAT280500.010.0593791
CCCGTCT181000.09.9002881
GTCCGGT111050.09.814331
Produced by FastQC (version 0.11.5)