FastQCFastQC Report
Mon 6 Apr 2020
Mlat_P_5A.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_P_5A.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37649935
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG893480.237312494696206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTCG98300.010.8038873
TAAGGCC173600.010.70831145
CACTCGA121950.010.1327274
CGGATAT212650.08.9230511
CTCGGCT169400.08.558681
CTCGCAT148300.08.4224088
GTCTGAC134850.08.2741991
TATCTCG137600.08.0579785
TCTCGCA169450.08.0018597
ATCTCGG160900.07.84588966
TCGCATC161500.07.81672959
ACTCGAA204150.07.78688865
ATATAGC165200.07.6012366
GCTCTCG207950.07.48399355
TCTCGGC145900.07.4164517
CTCTCGC225750.07.21935656
TCGAAGC311400.06.97109757
GTGCACT198300.06.90703341
TCGGCTC215700.06.8144842
CGGCTCT204650.06.78872163
Produced by FastQC (version 0.11.5)