FastQCFastQC Report
Mon 6 Apr 2020
Mlat_P_5B.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_P_5B.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43799025
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAG74200.028.5222078
GTGAATA369550.026.1243782
ACCTCGC137500.025.8120541
CGCTCAG147550.024.595195
TCGCCTA98450.021.8490817
ATAACTC447300.020.8025996
ACTCCTG500400.018.0681769
GAATAAC533800.017.639614
TGTGAAT541550.017.5962411
GCCTAGT121400.017.3756929
CGCGCTA44850.016.8636554
CCTCGCT228400.016.2690452
GTCCAGT360300.016.0577661
TAACTCC579750.016.0500077
TCGCTCA232550.015.7545244
AATAACT615800.015.5273475
TGAATAA645300.015.3988713
GTCCGCT102950.014.5049571
GTCCGCG74100.014.2472061
CTCGCTC261400.014.2287493
Produced by FastQC (version 0.11.5)