FastQCFastQC Report
Mon 6 Apr 2020
Mlat_P_5B.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_P_5B.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43799025
Sequences flagged as poor quality0
Sequence length20-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22667885.175430274988998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTCG125650.012.7510753
CACTCGA152650.012.0859874
AGATCGC184700.09.9887714
ACGAACG66200.09.9532053
ACTCGAA255150.09.8131615
CGCAAAC168750.08.91887958
ATATAGC179400.08.7760836
TCGAAGC371050.08.0937787
TATATAG189800.07.89323235
GTGCACT230450.07.79650071
CGAACGA94350.07.718714
GATCGCA231200.07.5297965
ATCGCAA240500.07.29630146
AGATAGC300750.07.03382446
GAAAATC727800.06.4052172
AAGATCG443000.06.2938973
CGCGCTA44200.06.27671059
GGGGAAT366000.06.27369741
TAAGGCC197500.06.2573023145
GGGAAAT607550.06.18862151
Produced by FastQC (version 0.11.5)