FastQCFastQC Report
Mon 6 Apr 2020
Mlat_P_5D.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_P_5D.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42458184
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAATA372650.033.3350452
ATAACTC459800.026.390736
ACCTCGC125350.026.3349041
ACTCCTG485450.024.7255739
CGCTCAG143900.023.5919555
GAATAAC530400.023.1907984
TGTGAAT544050.022.515011
CGCCTAG70950.022.5113098
TAACTCC557200.021.9759987
AATAACT625650.019.837015
TGAATAA641450.019.8344543
GTCCGAA154300.018.3888951
TCGCCTA93400.017.5445657
CGCGCTA37950.016.7617454
GTCCGCT89450.016.4792331
TCGCTCA213250.016.1791134
CCTCGCT220550.015.595062
GTCGCGT35150.015.1601371
CTCGCTC232500.014.9592643
GTCCAGT346600.014.9351891
Produced by FastQC (version 0.11.5)