FastQCFastQC Report
Mon 6 Apr 2020
Mlat_P_5D.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_P_5D.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42458184
Sequences flagged as poor quality0
Sequence length20-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22351645.264389075142733No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516380.12162083993041248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCGA146450.010.7698994
GCACTCG120100.010.5984173
AGATCGC168500.010.058484
ACGAACG62850.09.685983
CGCAAAC150100.08.9870688
ACTCGAA236000.08.9696525
TAAGGCC191400.08.750348145
GATCGCA220000.07.38943675
TCGAAGC344950.07.119317
AGATAGC291200.06.8655086
GGGGAAT339150.06.81405641
CGAACGA95050.06.76857144
ATCGCAA226800.06.6493576
AAGATCG384050.06.59264233
GGATAAT330000.06.520751
GGGAAAT580750.06.2906541
GTGCACT228050.06.28045851
GAAGATC953950.06.1796672
AGAAGAT1672850.06.1669691
GTAACGT50950.05.9752982
Produced by FastQC (version 0.11.5)