FastQCFastQC Report
Mon 6 Apr 2020
Mlat_T_2A.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_T_2A.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35146636
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAATA291400.027.4845982
ACTCCTG364300.021.1251039
ATAACTC376150.020.7705356
GAATAAC397800.019.9513114
TAACTCC390950.019.860987
TGTGAAT441950.017.8882541
TGAATAA515100.016.2766423
ACCTCGC94650.015.4246191
AATAACT543000.015.0848975
GTCCGAA133750.014.9314991
GTCCAGT255550.014.1745431
GTCCGCT76750.014.0870631
CGCTCAG113150.013.6759785
GCCCTAT83200.012.3328121
GTCGCAT84300.012.2535771
GTCCGCA124250.011.9162951
GTCCGTT85500.011.759421
GCCCAAT265500.011.7498651
GTCCGAT114350.011.5628331
GTCGCGT25100.011.5232441
Produced by FastQC (version 0.11.5)