FastQCFastQC Report
Mon 6 Apr 2020
Mlat_T_2A.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_T_2A.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35146636
Sequences flagged as poor quality0
Sequence length20-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7015191.995977652029059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG73150.022.2934653
CGAACGA100250.016.4729144
GAACGAA192100.011.8244991
AACGAAC156700.010.5863012
ACGAATC138950.08.3719052
AACGAAT157600.07.8616281
CACTCGA131950.07.61354644
GCACTCG113650.07.20478533
AGATAGC224800.07.16247466
CGAATCC198750.06.993393
GGGAAAT496600.06.5284541
GGGGAAT285400.06.48775531
AGATCGC130650.06.47797444
GAAAATC593850.06.4213912
ACTCGAA199300.06.3871625
TCGAAGC273900.06.380937
GGAAAAT934650.05.9432051
GGAACAT337000.05.84160661
GAAGATC772200.05.8207582
GGGAATC337750.05.78787472
Produced by FastQC (version 0.11.5)