FastQCFastQC Report
Mon 6 Apr 2020
Mlat_T_2B.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_T_2B.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42767512
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC133600.027.6038991
CGCTCAG151250.024.5001475
CGCCTAG75050.023.3884418
TCGCTCA223500.017.5150434
GTGAATA315050.017.4589752
CCTCGCT223050.017.1589742
TCGCCTA106400.017.086397
GCCTAGT111500.015.9300359
GTCCAGT325050.015.7422621
GTCCGAA164600.014.2731971
CTCGCTC290100.013.8547053
ATAACTC408050.012.8709456
GTCCGAT149750.012.8022731
GTCCGTT109200.012.6404851
GCCCTAT111250.012.5955531
TAACTCC417500.012.3626967
GAATAAC435400.012.2864164
GTCCTGT243200.011.9534621
GTCCGGA122750.011.9266641
ACTCCTG432450.011.8547759
Produced by FastQC (version 0.11.5)