FastQCFastQC Report
Mon 6 Apr 2020
Mlat_T_2B.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_T_2B.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42767512
Sequences flagged as poor quality0
Sequence length20-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14715083.4407145311609426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG84800.019.4688993
CGAACGA118500.014.4024584
GAACGAA228000.010.4511091
AACGAAC189900.09.3192272
ACGAATC165850.08.864172
CACTCGA166900.08.7232614
GCTAGTG298300.08.5953921
CTAGTGG282500.08.4348782
ACTCGAA244600.07.7179455
ACCTATT245250.07.67053941
GCACTCG139000.07.4672443
AACGAAT183000.07.31006571
AGATCGC160400.07.20929154
CCTATTG277300.07.16087532
TAGTGGA376700.06.63877153
CGAATCC246350.06.5885873
CGCAAAC133750.06.40616568
TCGAAGC346650.06.2697727
GAAAATC723150.06.1277332
GGATAAT355100.05.9647751
Produced by FastQC (version 0.11.5)