FastQCFastQC Report
Mon 6 Apr 2020
Mlat_T_2C.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_T_2C.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44659943
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAATA351300.023.7719042
ACCTCGC133900.022.513061
CGCCTAG73400.021.3252338
CGCTCAG153950.019.971765
GTCCGCT105850.019.4925651
ATAACTC430000.018.7046786
TAACTCC455900.017.4120587
GAATAAC466150.017.2691154
TCGCCTA104400.015.7962097
TCGCTCA217550.015.0645824
GTCCGAA177250.014.8762311
GTCCAGT347700.014.8654341
TGTGAAT564000.014.6456481
CCTCGCT219750.014.4180712
TGAATAA619300.013.8566923
GCCCTAT114300.013.7681031
GTCCGTT118000.013.75131
AATAACT624050.013.2355325
GCCTAGT118150.013.0707469
GCCCCCT133950.012.8448851
Produced by FastQC (version 0.11.5)