FastQCFastQC Report
Mon 6 Apr 2020
Mlat_T_2C.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMlat_T_2C.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44659943
Sequences flagged as poor quality0
Sequence length20-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20132134.507871852859284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG85000.017.9993113
CGAACGA118300.013.4051544
GAACGAA233950.09.8264561
AACGAAC193300.08.9648682
ACGAATC167700.08.6250452
AACGAAT187000.08.4074631
GCTAGTG300700.08.0634361
CTAGTGG298100.07.85248142
AGATCGC165050.07.53416544
CGCAAAC148200.06.45804558
TAGTGGA385200.06.20255333
GGGGAAT351500.06.04327441
CGAATCC252400.06.0338963
CACTCGA176250.05.9455614
ACTCGAA251350.05.9201325
GGGAAAT606000.05.74223231
GATCGCA201200.05.7291035
CGCAACT140250.05.7282997
GCGAGTT187450.05.704695145
TAAATCG113100.05.6209473
Produced by FastQC (version 0.11.5)