FastQCFastQC Report
Mon 6 Apr 2020
Mter_P_4A.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_P_4A.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34470728
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC115950.037.1726531
CGCCTAG73450.036.3452538
CGCTCAG125900.035.120725
GTGAATA296350.034.0296672
TCGCCTA83900.031.8182967
ATAACTC361600.027.6611776
GCCTAGT98400.027.3399739
ACTCCTG369650.026.7787159
GAATAAC405050.024.9664274
TAACTCC406850.024.5166917
TGTGAAT435150.023.5244221
TCGCTCA195550.023.070184
CCTCGCT212150.020.7073172
CTCGCTC220450.020.5909843
TGAATAA511850.020.4323273
AATAACT517150.019.9680825
ATGCTCG154350.017.25213
CTCGCCT166000.016.9544286
TGCTCGC158900.016.8003434
GTCCGAA132450.015.3843731
Produced by FastQC (version 0.11.5)