FastQCFastQC Report
Mon 6 Apr 2020
Mter_P_4A.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_P_4A.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34470728
Sequences flagged as poor quality0
Sequence length20-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10631653.0842545594047217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAAC159500.014.5895115
TAACTAC163100.013.2513868
CTAGTGG223050.012.6348282
GCTAGTG247100.012.2716571
TTAACTA193200.011.6157647
CTTAACT215550.011.0520446
ACGAACG52450.010.6637773
AGATCGC138100.010.2501854
AACTTAA281500.09.8854894
CGCAAAC123150.09.7002428
TAGTGGA300350.09.6099293
CAACTTA278200.09.2333093
CACTCGA136100.08.9802134
GCACTCG105400.08.9098393
TCCCGTA121700.08.170398
AGATAGC221300.08.1126636
GATCGCA172750.08.03432755
ACCTATT185300.07.64169741
ACTCGAA213850.07.555765
GCAACTT410800.07.5328442
Produced by FastQC (version 0.11.5)