FastQCFastQC Report
Mon 6 Apr 2020
Mter_P_4B.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_P_4B.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41308220
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC125750.030.5968271
CGCTCAG141200.027.3681135
CGCCTAG82100.025.6893588
TCGCCTA97450.021.5716617
GCCTAGT110400.018.7270769
GTGAATA305700.018.4928382
TCGCTCA221850.018.0443294
CTCGCTC250450.016.4279063
CCTCGCT245400.016.2164082
ATAACTC378900.014.4835916
GTCCGAA151650.014.2885051
GTCCGCT96550.013.5232221
GTCCAGT313050.013.3554141
GAATAAC425300.013.2133834
GCCCTAT104500.012.8267191
TAACTCC433350.012.6636957
ACTCCTG430950.012.4283439
GTCGGTT128450.011.8408981
GCCCGTT113450.011.8148281
ATGCTCG181250.011.7519223
Produced by FastQC (version 0.11.5)