FastQCFastQC Report
Mon 6 Apr 2020
Mter_P_4B.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_P_4B.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41308220
Sequences flagged as poor quality0
Sequence length20-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17213574.167105239586697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAAC177800.012.3744825
TAACTAC183650.011.2621478
ACGAACG59150.011.0299273
TTAACTA210550.010.5485497
CTTAACT232800.09.7490966
GCACTCG130250.09.4318113
CACTCGA159050.09.2512784
CTAGTGG253150.08.7482172
ACTCGAA255800.08.384125
GCTAGTG287600.08.3762071
CGAACGA88150.08.3460914
CAACTTA320950.07.9581113
AACTTAA323400.07.8334384
TCCCGTA134300.07.5452348
CCCGTAA146150.07.36086379
AGATCGC148950.07.0361654
ACGAATC129550.06.9128822
TAGTGGA353150.06.8197723
GTGCACT216800.06.7246061
GGATAAT317250.06.6742891
Produced by FastQC (version 0.11.5)