FastQCFastQC Report
Mon 6 Apr 2020
Mter_P_4C.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_P_4C.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43067539
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC118900.024.3664471
CGCTCAG138400.021.6083645
GTGAATA302600.015.7372622
CGCCTAG77900.015.3560148
TCGCTCA213800.014.1486084
TCGCCTA92350.013.2510357
CCTCGCT232550.013.1092952
CTCGCTC248350.012.5682573
GTCCGAA155050.012.2054241
GTCCGCT94150.012.0602291
GTCCAGT314300.011.8890561
GCCCTAT99750.011.659121
ATAACTC397900.011.5935736
GTCGCAT104300.011.4144171
GTCCGCA139500.011.2474121
GCCCAAT336800.010.9926431
GAATAAC442350.010.6150834
GTCCGAT144900.010.5907921
ACTCCTG421100.010.4323559
CCCGTAT124800.010.3665731
Produced by FastQC (version 0.11.5)