FastQCFastQC Report
Mon 6 Apr 2020
Mter_P_4C.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_P_4C.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43067539
Sequences flagged as poor quality0
Sequence length20-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17944024.166483717585999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAAC181800.012.3783775
TAACTAC185950.011.1768418
ACGAACG57350.010.4398833
TTAACTA223350.09.8907427
GCACTCG130150.09.2534513
CTTAACT249900.09.1428526
CACTCGA158600.09.1122574
AACTTAA343250.08.1199674
CGTATGC124950.08.0981671
TCCCGTA132950.07.9715128
ACTCGAA262750.07.96233945
CAACTTA339800.07.7973533
GCTAGTG296550.07.1724281
CTAGTGG267050.07.11413532
CGAACGA87200.07.10290244
CCCGTAA152250.06.91580069
GCAACTT497400.06.86406762
GGGGAAT321600.06.7207731
ACGAATC133000.06.52115542
GGAAAAT1043950.06.36946631
Produced by FastQC (version 0.11.5)