FastQCFastQC Report
Mon 6 Apr 2020
Mter_T_1A.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_T_1A.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41433605
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC127750.031.7877451
CGCCTAG83500.031.5956258
TCGCCTA103700.026.3000777
GCCTAGT113550.023.2341239
ACGGATA140300.022.0767466
GTGAATA306550.021.2579422
CGCTCAG200000.021.2087445
TCGCTCA208050.020.0916884
CCTCGCT209000.019.9223842
GTCGCGT56950.019.1514821
CTCGCTC258200.016.7474583
TGCTCGC166100.016.172264
ATGCTCG168400.016.1556423
CGGATAT195100.016.016237
ATAACTC405850.015.6350656
GAATAAC421850.015.2694754
CTCGCCT181250.015.2363626
TAACTCC412100.015.2316027
GTCCGCT98150.015.1659061
GTCGCAT94300.015.1304061
Produced by FastQC (version 0.11.5)