FastQCFastQC Report
Mon 6 Apr 2020
Mter_T_1A.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_T_1A.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41433605
Sequences flagged as poor quality0
Sequence length20-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13175953.1800153522726298No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441570.10657291345998011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTCG120950.09.9744733
CACTCGA155350.09.7513384
GTCTGAC202600.09.47547151
CTAGTGG263600.08.739292
GCTAGTG277650.08.6674571
ACGAATC137000.08.1573532
ACTCGAA224100.07.9221145
TCGAAGC315900.07.6162227
ACGAACG121250.07.57541133
TTACCCG143500.07.5472258
TACCCGC139650.07.41170559
ACGCGAC39903.45608E-117.3318145
GTAGTCA134850.06.9130568
TACCCTA129850.06.86251839
CGCCCGC151650.06.825832145
AGATCGC137800.06.8148224
GGGGAAT346300.06.81064461
ACATGTG286350.06.77442466
GTGCACT214750.06.70453071
GGATAAT323500.06.67603351
Produced by FastQC (version 0.11.5)