FastQCFastQC Report
Mon 6 Apr 2020
Mter_T_1B.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_T_1B.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43156157
Sequences flagged as poor quality0
Sequence length20-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGT99850.031.5053621
ACCTCGC117850.028.4650231
GTCCGCT97300.023.3899361
GCGTATT157250.019.0591144
CGCCTAG74150.018.661958
ACGCGTC260550.018.465015145
CCTCGCT189850.018.036352
TCGCTCA195750.017.5840744
GCCACGC277150.017.0258541
TCGCCTA93850.015.6337557
CTCGCTC232600.015.0080813
GTCCAGT306050.014.9178281
ACGCTTT307550.014.6060534
TACTACC366100.014.5119173
GTCCGAA156950.014.2343571
GTTCGAT322050.014.00379851
GTCGCAT92750.013.8069581
GCCACTT427000.013.7728221
GCCCAAT299850.013.7640031
ACGGATA145900.013.7262946
Produced by FastQC (version 0.11.5)