FastQCFastQC Report
Mon 6 Apr 2020
Mter_T_1B.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_T_1B.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43156157
Sequences flagged as poor quality0
Sequence length20-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32622007.559060460364902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCGG144350.013.1515414
ACATGTG336200.012.8240376
GTCTGAC347850.012.6365881
TACCCGC190400.012.1985819
TGTGTGC347300.012.1938919
TTACCCG195350.012.1742568
TCAGGCG159300.012.0919073
CATGTGT366500.011.7068877
TACCCTA190350.011.3615429
CTGACAT378250.011.2145463
GGAGTCT351550.011.1978361
GACATGT393100.011.1623885
GTCAGGC189200.010.6238392
CGGGATT218550.010.3728373
GTGTGCG202000.010.15547910-14
TCTGACA416950.010.0419322
ATGTGTG425700.09.81749258
GCTAGTG265300.09.69994451
CGGAGCG230050.09.634105145
ACCCGCT177000.09.4290469
Produced by FastQC (version 0.11.5)