FastQCFastQC Report
Mon 6 Apr 2020
Mter_T_1D.R1.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_T_1D.R1.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40419021
Sequences flagged as poor quality0
Sequence length20-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA524340.12972605150431527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC106950.025.0262071
GTCCGCT97100.023.880141
CGCTCAG126400.021.8058825
CGCCTAG66500.017.5711358
CCTCGCT180450.015.6333912
GTCCAGT301200.015.1455411
GTGAATA293300.015.0373822
GCCCTAT96250.014.7977551
GCCCAAT310900.014.6951351
TCGCTCA193900.014.5339084
GTCCGAA155950.014.1626951
GCCACAT465700.013.6370911
TCGCCTA91500.013.5965927
GTCCATT437150.013.2370661
GCCGTAT100000.013.210751
GTCGCGT28150.013.1990181
GCGTTGC161450.013.1977535
GTCCGCA138250.013.1888511
GCCCGTT112500.013.0272671
GTCGCAT96300.012.5751331
Produced by FastQC (version 0.11.5)