FastQCFastQC Report
Mon 6 Apr 2020
Mter_T_1D.R2.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMter_T_1D.R2.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40419021
Sequences flagged as poor quality0
Sequence length20-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37495369.276662094314457No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1071630.2651301227706629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTCG109400.09.870533
CACTCGA142000.09.0575644
ACGAACG61850.08.2290583
GCTAGTG255800.08.0950151
CTAGTGG245450.08.0159442
TCGAAGC285350.07.4479757
ACTCGAA212650.07.43910365
GGGGAAT295200.07.20101641
GGGAAAT528700.07.143561
GTGCACT203950.06.84737251
GGATAAT308050.06.54331831
GAAGATC784100.06.40476272
CGAACGA87500.06.36700444
GGAAGAT911100.06.34256461
GAAAATC633800.06.28460552
GAACATC290700.06.2712272
GGAACAT368150.06.24127531
AGTCGAA186300.06.23924455
AGACAAT405450.06.2100551
GGACAAT348450.06.17953161
Produced by FastQC (version 0.11.5)