FastQCFastQC Report
Sat 15 Feb 2020
S5.R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS5.R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25414580
Sequences flagged as poor quality0
Sequence length25-75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTT3001311.1809402319455997No Hit
CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGC1659230.6528654024579592No Hit
GTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAAC1581640.6223356829032783No Hit
ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTGAATACATCAGTGTAGCGC1194330.4699389090828965No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCG1152170.45335000617755633No Hit
CCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCT1148950.4520830169139132No Hit
CGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT1073870.4225409194249915No Hit
CTTCGCTTTAGCTCTTAAGCTCTCTTCTTTCTGCTTCCTGATATTGTCTAACAGGGTACGTGCGTAACTTGACGC959100.3773818020994248No Hit
CCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG932470.36690356480414No Hit
GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA858700.3378769194690607No Hit
GCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTG842780.33161279863763243No Hit
CCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC830980.3269697945037848No Hit
CGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATT782960.30807512852858476No Hit
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCC772990.3041521835104101No Hit
GTCAGTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGG771380.3035186888785886No Hit
CTCCACGTAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTA759820.29897011872712437No Hit
CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC731570.287854452050752No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTTCCACC730280.2873468693954415No Hit
CGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC718870.28285732048296686No Hit
CCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTC667870.262790099226507No Hit
ATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGT644730.25368508942504653No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCG619250.24365934829534858No Hit
CTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTG616040.2423962937809714No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCC605180.23812315607812523No Hit
CCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTGAATACATCAGTGT568600.22372984326319775No Hit
GCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAGCGACCGCCGCG560650.2206017175967496No Hit
GCCCCCAATGCCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTT554930.2183510410166133No Hit
CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCT553660.2178513278598348No Hit
CTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAAC536860.21124094909300092No Hit
CCCCAATGCCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCG530850.20887616478415144No Hit
CTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCG526360.2071094623637298No Hit
CGAGGCTTTGCCACACGCAGCCCGTCTGTGGCAGTATAGTATTCGTCGGTTTCGCAACCGGGACTTTCAACGCCG503320.19804380005492908No Hit
CTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAAT491160.1932591449475065No Hit
CCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGT484810.19076057916361397No Hit
CTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGCCC475550.1871170013433234No Hit
CCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCTCAGA454900.17899174410909013No Hit
CTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACC445810.1754150570263211No Hit
CCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGC437120.171995759914191No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTT436130.17160621973685972No Hit
CCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAA429700.16907617595883936No Hit
GCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTT422110.16608970126596623No Hit
GTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTC420950.16563327035111342No Hit
CCTAGGTTTTGCAGCGACCGCCGCGCCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGT415090.1633275072812535No Hit
GTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCC410600.16156080486083185No Hit
CCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGC408400.1606951600223179No Hit
GATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATT399120.1570437127034954No Hit
GTTGGAATTGCGAGTTCGGGGATTTTTGCGATGAACTGAGAGCTCAACCCCAATCCTTCGATTTTTCTTCCATCT398960.15698075671523984No Hit
CCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGG391500.15404543376282434No Hit
CTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACT390250.15355359010457778No Hit
CCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTAT386130.1519324734069971No Hit
GTCCTTTCGCAGCATCTTTCGCCAGAATACTCCGGGGGTAACGTCCAGCAAATATACATGCTTGGGTAGCATCAT379840.14945751611870037No Hit
CACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCC379440.14930012614806146No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTCAC378260.1488358257346767No Hit
GTTCAGTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGCGCGATGACCAATTGTGCGA372810.14669138738472168No Hit
CTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGC370150.145644744079973No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTCACCC368510.1449994452003535No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC367130.1444564498016493No Hit
GGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCC364930.14359080496313534No Hit
CCCGAGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATT364040.14324061227846377No Hit
GCCAGTTTCTGCGTTCTTCTCGAATTCAGGCAACGGTTTTGGTGTTGGAATTGCGAGTTCGGGGATTTTTGCGAT357870.14081287198135872No Hit
GGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG357020.14047841829375107No Hit
CCCGCCTCCGATTCACGGAGTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTGCGCCGAAGCTCCCACTT345390.13590230489742502No Hit
CCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTA341050.13419462371599294No Hit
CCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTT340650.13403723374535403No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTT338930.13336045687160678No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTC338030.13300632943766924No Hit
CCGAGGCTTTGCCACACGCAGCCCGTCTGTGGCAGTATAGTATTCGTCGGTTTCGCAACCGGGACTTTCAACGCC333180.13109797604367257No Hit
CACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCG332730.1309209123267038No Hit
CACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAGCGACCGCCGCGCCCTCCT329360.12959490182407107No Hit
GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGC329090.1294886635938898No Hit
CTCGACTTTCCCAGTTAGCTTATGGTGACGGAAAACAAACGACGGATTCCTTGAGACAAAAGGAATTTGTTCCGT327730.12895353769371753No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCA323910.12745046347411604No Hit
GTCCCGACAGGCATGCTCACACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATC323710.12737176848879658No Hit
GTCGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGAACG322680.12696648931440144No Hit
ATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACT318370.12527061238076725No Hit
GTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGCGCGATGACCAATTGTGCGAATCAAC311870.12271302535788511No Hit
CTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTT308850.12152473107956141No Hit
CTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGC307180.120867627952144No Hit
GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCC301860.11877434134264662No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCTCGATTCCGTGTGACGGCTCAACAAAGC301560.11865629886466744No Hit
GTCGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCC298400.11741291809662013No Hit
CTCCTACTGAGGGTCGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTC297610.1171020729046083No Hit
CCAAGGTTCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGC293290.11540226122170817No Hit
CTCCCATCTAACATTGAAGTTAGGAATTTCCCCCTCGTTATGAATTAACATATCTGCATCAACCATCATGGTGC292890.11524487125106928No Hit
CACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGC292700.11517011101501579No Hit
GCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCT287040.11294304293047534No Hit
CTCACACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGCCAATCAGCTTC285800.11245513402149475No Hit
GTCGGTTTCGCAACCGGGACTTTCAACGCCGGAGGCTTCGGCAGTTGAGCTTTGCGTAGAGCAGCATAGTTCTG282490.11115273201445783No Hit
CCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG281920.11092845130629742No Hit
CTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAGCGACCGCCGCGC281030.11057825862162585No Hit
CTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGG279400.10993689449127232No Hit
GTCCCGGTTCCGGAATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGGGTTTCCCCCG278240.10948046357641952No Hit
CCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGCTCCGTTCCGCATCCGACCAGGACGC275040.10822134381130832No Hit
CCCGAGGCCCCCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTA274320.1079380418641583No Hit
GTTTTGTTGCGTGGCGTCCAGGGAAAAGGATATCTTCAAGACTTCACCTTTGTCTGAAGGGAGAGCATCCTGGGC274050.10783180363397704No Hit
CCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGC271870.10697402829399501No Hit
GTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTAT270130.10628938192171579No Hit
CTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGC268990.10584082050539494No Hit
GTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCC265420.10443611501744275No Hit
CACGTAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTA264660.10413707407322882No Hit
CCTCCACGTAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACT263380.10363342616718435No Hit
ATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCG262640.10334225472150237No Hit
CCCGTCTGTGGCAGTATAGTATTCGTCGGTTTCGCAACCGGGACTTTCAACGCCGGAGGCTTCGGCAGTTGAGCT261600.10293304079784123No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATT260560.10252382687418009No Hit
CTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGC258850.10185098474969878No Hit
CTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGC258380.10166605153419808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACGC168850.066.079869
GTCCGGT24100.063.0624241
GTCCCCT9550.049.8430751
GTCCCCA21250.048.1601371
GCCCTTT22450.048.009051
GATGTGC410250.046.3574169
AGTATGG18600.046.2474441
TGGATGC19450.045.6224755
ACTGGAT19750.045.100913
CCCGGTA150700.044.8050042
AACTTGA183850.044.703666
GTTCCGT84950.044.0022769
CGGGGGA21300.043.56713510
TAACTTG192300.042.43567365
GTACTGG21000.042.419171
TGTTCCG66200.042.4148268
CTTGACG205600.042.1150768
GCGTAAC196100.041.36284662
TGCGTAA196750.041.10136861
GCCCTCT21000.040.4763071
Produced by FastQC (version 0.11.5)