FastQCFastQC Report
Sat 15 Feb 2020
S6.R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS6.R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26065327
Sequences flagged as poor quality0
Sequence length24-75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTT921490.3535309570449663No Hit
ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTGAATACATCAGTGTAGCGC776330.29784011533789695No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTTCCACC697420.26756618092686885No Hit
GTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCT622350.23876546801043394No Hit
CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGC582630.2235268331757357No Hit
GTTCAGTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGCGCGATGACCAATTGTGCGA548750.21052872269739795No Hit
CCCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATTC535830.20557194621038133No Hit
GTCAGTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGG531950.20408337865855278No Hit
GTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAAC528750.20285569407972517No Hit
GTCGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCC481240.18462841459844337No Hit
CCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCT464130.17806413861602427No Hit
CCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTGAATACATCAGTGT401100.15388258892742837No Hit
CTCAAACTTCCTTGGCCTAAACGGCCATAGTCCCTCTAAGAAGCCGGCCGTGAAGGGATGCCTCCACGTAGCT365600.14026296313105913No Hit
CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGGTTCGAGTGGCATT350100.1343163659523627No Hit
GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA343050.131611623364633No Hit
GTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTC341200.13090186821749827No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCG333770.12805133808603283No Hit
CGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATT328070.12586452492999609No Hit
GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTGAAGACCAAC327230.12554225772805383No Hit
CTCTAAGAAGCCGGCCGTGAAGGGATGCCTCCACGTAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAAT313810.12039365552559537No Hit
CTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGC306850.11772344156664523No Hit
CCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC306340.11752777933689455No Hit
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCCCGAGAGAGCCGTTCCCAGTCCGTCC298850.1146542301195761No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATTCTGC290360.11139702947137399No Hit
GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGGTTCGAGTGGCATTTCACCCC288360.11062972660960671No Hit
GTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTTATCTAATAAATGCGTCCCTTCC280860.1077523408779794No Hit
GTTCAAAGACTCGATGGTTCACGGGATTCTGCAATTCACACCAAGTATCGCATTTCGCTACGTTCTTCATCGATG279500.10723057493197763No Hit
CCTTCCTCTAAATGATAAGGTTTAGTGGACTTCTCGCGACGTCGCGGGCGGCGAACCACCCACGTCGCCGCGATC278700.10692365378727071No Hit
CTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATCTCTACG278690.10691981727296189No Hit
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCC275410.10566144057966355No Hit
GGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCC271940.10433017011449731No Hit
CTCACACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGCCAATCAGCTTC266310.10217021255862241No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTT264160.1013453619822226No Hit
CTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGC263910.10124944912450168No Hit
CTTCGCTTTAGCTCTTAAGCTCTCTTCTTTCTGCTTCCTGATATTGTCTAACAGGGTACGTGCGTAACTTGACGC262810.10082743255052967No Hit
GTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCC262780.10081592300760316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACGC68800.049.2537769
GTCCGGT15050.048.245691
CTGGCGT22150.046.0136031
GTCCCCT12250.045.9503821
GTGACGC13200.043.9049841
CGGGGGA15100.040.57586310
GTCCCGT6750.038.9817961
GTCCATT23400.038.8584371
TTGGGCT60600.038.71986469
TGACGCC15200.038.564122
GTCCCCA19900.038.328421
CCAGGCA146450.037.7286572
TTTGGGC48250.036.73781268
CCGGTCT20450.036.3203541
GCCCTCT15500.036.3156241
GGTCCCT11000.036.0323681
TGGGGGG28500.035.060241
GGCGTCG29650.035.045533
GATGTGC220600.034.84039369
TGGATGC17850.034.704235
Produced by FastQC (version 0.11.5)