FastQCFastQC Report
Sat 15 Feb 2020
S6.R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS6.R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26065327
Sequences flagged as poor quality0
Sequence length23-75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTG603940.23170244516786612No Hit
GTTCGGATCGCGGCGACGTGGGTGGTTCGCCGCCCGCGACGTCGCGAGAAGTCCACTAAACCTTATCATTTAG540970.20754391456512325No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCG483700.1855721971184171No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCG447470.17167250577750282No Hit
CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGC424920.16302116601107672No Hit
ATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG415180.15928440107427005No Hit
CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCACACCTTGGCCGACAGGCCCGGGT378170.1450854616172665No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATC378020.14502791390263395No Hit
CGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGATTATG376380.14439872555598476No Hit
ATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCAT371130.14238455554384566No Hit
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCAC370160.14201241365588854No Hit
GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCACACCTTGGCCGACAGGCCCGGGTAATCTTT364080.13967981295611598No Hit
CTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGG355710.13646865047961992No Hit
CTTGAAAATCCGGAGGACCGAGTGCCGCTCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAAC336050.12892606334844753No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACG313800.12038981901128652No Hit
GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGGACGGGAAGTGGTGTTTCC299810.11502253549322439No Hit
GGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTC296710.11383321605748511No Hit
CGAACAGTCAGCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCC282270.10829328939552534No Hit
ATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTT267480.10261908473275627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTAG101400.051.52345767
GATCCTG68000.048.453335
ATCCTGG68700.047.620436
CCTGCCA47050.043.5029451
CCTGGCT76050.042.405478
CTGCCAG49100.041.4829642
GCCAGTA49600.040.9976124
CTTATCA124150.040.9205862
CTGGCTC79300.040.7933739
TGCCAGT50900.040.1467553
TCCTGGC88050.037.1504447
ATCATTT144850.036.07153765
TCATTTA144300.035.66127866
GCAACGG81000.035.5000153
GGCTTAC40800.034.994541
GTATGGC17800.034.2161332
GCTCAGG89400.034.13782512
CCTTATC149250.033.80230761
GCTTACG42750.033.086642
GTCCTCG22300.032.817566
Produced by FastQC (version 0.11.5)