FastQCFastQC Report
Thu 20 Feb 2020
Mu.S1.1.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S1.1.R1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences21566555
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG181800.088.239952
GAGTACG207700.078.422481
GTACGGG257700.061.299953
TACGGGG139650.054.7952654
TACGGGA118900.038.2390334
TACGGGT58250.033.2748264
TACGGGC70150.029.170924
ACGGGGG84250.026.1495025
ACGGGGT72450.020.0735845
ACGGGGA143350.019.1856985
ACGGGGC76400.018.86563
ACGGGAC67950.016.8632643
GTATAAG86700.015.468731
ACGGGAG115350.015.1046155
GTATAGA81400.014.3499841
TATACTG139850.014.2603185
ACGGGAT85250.014.103735
TATACAG172200.013.9644955
GTATATA139400.013.0346261
TACACTG190150.012.7598495
Produced by FastQC (version 0.11.5)