FastQCFastQC Report
Thu 20 Feb 2020
Mu.S1.1.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S1.1.R2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences21566555
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG181800.088.70542
GAGTACG212150.077.333431
GTACGGG257050.062.092623
TACGGGG144700.057.417554
TACGGGA112300.037.8578642
TACGGGT57700.034.3431974
ACGGGGG79450.029.542145
TACGGGC71500.028.0170634
ACGGGGA136300.021.4989245
ACGGGGT76950.021.1455025
ACGGGGC81350.019.5592825
ACGGGAC67600.015.66947653
GTATAAG82900.015.564551
TATACAG166900.013.8473585
TATACTG135900.013.6684265
ACGGGAG114100.013.2622593
CTATACT89850.013.0723424
CCTATAC71950.012.9194133
TACACTG197000.012.7914565
GTATAGA82200.012.4524441
Produced by FastQC (version 0.11.5)