FastQCFastQC Report
Thu 20 Feb 2020
Mu.S1.2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S1.2.R1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20821445
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG146450.075.468412
GAGTACG172550.064.944861
GTACGGG197500.055.12223
TACGGGG105950.050.9978564
TACGGGA100000.031.6989044
TACGGGT47900.025.869294
TACGGGC63250.022.3247784
ACGGGGG80050.016.8188155
GTATAGA72700.015.6621741
ACGGGAC66550.015.4816463
ACGGGGA139850.015.4445035
TATACTG123850.014.998195
ACGGGGC71700.014.9617445
GTATAAG79150.014.2037521
GTATATA118200.014.083931
CGTATAC29000.013.912953
TATACAG144550.012.9500195
ACGGGGT74000.012.9400235
TACACTG167150.012.6204955
CTATACT81100.012.6141824
Produced by FastQC (version 0.11.5)