FastQCFastQC Report
Thu 20 Feb 2020
Mu.S1.2.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S1.2.R2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20821445
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG145900.076.149132
GAGTACG166400.067.742671
GTACGGG196750.055.4796563
TACGGGG107900.051.0829354
TACGGGA95550.031.821282
TACGGGT52500.027.173264
ACGGGGG69700.022.7251935
TACGGGC58450.021.5719494
ACGGGGA133300.015.8794285
TATACTG124700.015.3579365
ACGGGGT72150.014.9683045
ACGGGGC75950.013.9452213
CGTATAC31350.013.3298833
GTATAGA69650.012.93503951
TACACTG173200.012.8448275
CTATACT81550.012.7225574
GTATAAG75800.012.6462371
GTATATA116750.012.5936651
TATACAG138650.012.5145385
ACGGGAC65200.012.1557273
Produced by FastQC (version 0.11.5)