FastQCFastQC Report
Thu 20 Feb 2020
Mu.S1.3.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S1.3.R1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22203674
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG198000.088.861662
GAGTACG218350.081.521581
GTACGGG271600.063.3222853
TACGGGG146950.057.4542924
TACGGGA121750.038.5191274
TACGGGT61550.034.2928854
TACGGGC79350.028.0527864
ACGGGGG83800.026.7195685
ACGGGGA155400.022.2847185
ACGGGGT73900.019.6797685
ACGGGGC77850.018.8662075
ACGGGAC74550.016.1399733
GTATAAG86600.014.7293731
GTATAGG62650.014.378641
TATACTG142350.014.2122195
ACGGGAG109600.014.1234565
TACACTG198000.013.6721145
TATACCG48750.013.2917125
CTATACT90050.012.7197294
CCTATAC73350.012.6713693
Produced by FastQC (version 0.11.5)