FastQCFastQC Report
Thu 20 Feb 2020
Mu.S1.3.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S1.3.R2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22203674
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG196950.092.330742
GAGTACG223050.082.281
GTACGGG280850.063.5425343
TACGGGG148500.056.763924
TACGGGT63700.040.4908184
TACGGGA127550.038.9181064
ACGGGGG77800.029.5209665
TACGGGC79850.026.7975124
ACGGGGA147300.022.5818065
ACGGGGC84250.019.3264223
ACGGGGT77350.019.0815225
ACGGGAC74850.018.480873
ACGGGTG77400.016.7436835
TATACTG143300.015.5752485
CGGGACC47800.014.1681424
ACGGGAG113250.013.3505935
TATACAG177450.012.9429765
TACACTG195500.012.889645
GTATAAG86850.012.6987121
CCTATAC69350.012.6743693
Produced by FastQC (version 0.11.5)