FastQCFastQC Report
Thu 20 Feb 2020
Mu.S2.1.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S2.1.R1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20061074
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG136550.070.2639852
GAGTACG159150.061.961281
GTACGGG184400.050.507733
TACGGGG99650.047.476114
TACGGGA96550.032.5277822
TACGGGT45400.019.667674
TACGGGC57450.017.2972134
GTATAAG75700.015.9023571
ACGGGGG77300.014.9958365
TATACTG117100.014.7563395
GTATATA110450.014.5539231
ACGGGGC73850.014.3379513
ACGGGGA139000.014.1925385
GTATTAG63450.013.2921491
CTATACT80150.013.1170574
TAGACTG71350.012.9163655
TATACCG37650.012.8125085
TATACAG135850.012.6666735
GTATTAA68000.012.6147591
ACGGGAC66250.012.6122433
Produced by FastQC (version 0.11.5)