FastQCFastQC Report
Thu 20 Feb 2020
Mu.S2.1.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S2.1.R2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20061074
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG137050.076.176152
GAGTACG161950.065.530971
GTACGGG191500.053.388733
TACGGGG102300.050.3926774
TACGGGA95900.031.9831314
ACGGGGG69600.022.3447575
TACGGGC60000.021.8398764
TACGGGT49050.020.2567654
TATACTG118550.015.4263535
ACGGGGC73900.014.0297255
ACGGGGA137300.013.7916535
GTATATA111250.013.5909341
CTTACAC101200.013.3754183
CTATACT77900.013.216874
GTATAGG55350.013.0080561
TCTATAC67150.012.8666433
TACACTG159650.012.6275825
TATACAG133500.012.4583865
GTATTAC74900.012.4004641
TAGACTG72450.012.3229425
Produced by FastQC (version 0.11.5)