FastQCFastQC Report
Thu 20 Feb 2020
Mu.S2.2.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S2.2.R1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20322651
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG148650.073.8912352
GAGTACG165650.067.650291
GTACGGG200300.053.3630873
TACGGGG105000.049.1720664
TACGGGA104500.032.1802334
TACGGGT49350.023.054654
TACGGGC59400.021.8214
TATACTG117050.016.8540255
ACGGGGG79050.016.4854645
GTATAAG72600.015.4852671
ACGGGAC66550.015.3683863
ACGGGGA144400.014.7587315
GTATATA118600.014.5833471
TATACCG37900.014.4377285
CCTATAC62900.014.4280593
GTATTAG68900.014.2249371
ACGGGGC79550.013.5761175
TACACCG98500.013.52259355
TCTATAC72150.013.4767583
GTCCTAG59100.013.4133081
Produced by FastQC (version 0.11.5)