FastQCFastQC Report
Thu 20 Feb 2020
Mu.S2.2.R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S2.2.R2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20322651
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG151700.078.2196662
GAGTACG174550.069.181271
GTACGGG206550.056.6812743
TACGGGG103700.054.296564
TACGGGA105850.035.16774
TACGGGT47200.024.7125364
TACGGGC62950.023.3334394
ACGGGGG74100.019.9188845
ACGGGGA143050.016.1564735
ACGGGGC80650.015.8014985
TATACTG114050.015.1511395
ACGGGAC70200.014.2567983
GTATAAG72400.013.9240251
CTATACT76600.013.1596274
ACGGGAG111600.012.96764855
GTATAGG58150.012.7544811
TCTATAC69900.012.6698723
GTATATA117050.012.61123851
GTCTAGA71800.012.5360551
TACACTG170050.012.5326945
Produced by FastQC (version 0.11.5)