FastQCFastQC Report
Thu 20 Feb 2020
Mu.S2.3.R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMu.S2.3.R1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22179107
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG154250.072.992692
GAGTACG180300.063.9708941
GTACGGG209200.053.2689553
TACGGGG111000.050.6020744
TACGGGA108150.031.8271714
TACGGGT51300.023.161324
TACGGGC65150.019.4533754
ACGGGGG85800.016.3632895
ACGGGGA157300.015.0588035
TATACTG127300.014.9878975
GTATATA123600.014.289131
ACGGGAC71250.014.2554133
CTATACT84400.014.163234
ACGGGGC81100.013.8492745
TAGACTG79350.013.519565
CCTATAC64900.013.3193223
CTTACAC113550.012.8147393
TACACTG183100.012.6616645
TATACAG149850.012.4922275
TACACCG113350.012.386155
Produced by FastQC (version 0.11.5)